Dually Responsive Injectable Hydrogel Prepared by In Situ Cross-Linking of Glycol Chitosan and Benzaldehyde-Capped PEO-PPO-PEO

Ding, Chen; Zhao, Lingling; Liu, Fuyong; Cheng, Jun; Gu, Jingxia; Shan, Dan; Liu, Chenyang; Qu, Xiaozhong; Yang, Zhenzhong

doi:10.1021/bm1000179

Cited by 128 publications

(111 citation statements)

References 36 publications

Supporting

Mentioning

105

Contrasting

Order By: Relevance

“…In the tested frequency range, the storage modulus was found to be independent of frequency indicating the stability of the crosslinked hydrogels. 52 Further, the storage modulus was found to significantly increase with increasing polymer concentrations. The 0.5% gels showed a lower modulus of about 145 AE 2 Pa compared to the 2% and 4% gels with modulus of 2756 AE 35 Pa and 5520 AE 269 Pa respectively.…”

Section: Resultsmentioning

confidence: 93%

Evaluation of enzymatically crosslinked injectable glycol chitosan hydrogel

et al. 2015

View full text Add to dashboard Cite

Enzymatically cross-linkable phenol-conjugated glycol chitosan was prepared by reacting glycol chitosan with 3-(4-hydroxyphenyl)propionic acid (HPP). The chemical modification was confirmed by FTIR, 1 H-NMR and UV spectroscopy. Glycol chitosan hydrogels (HPP-GC) with or without rhBMP-2 were prepared by the oxidative coupling of the substituted phenol groups in the presence of hydrogen peroxide and horse radish peroxidase. Rheological characterization demonstrated the feasibility of developing hydrogels with varying storage moduli by changing the polymer concentration. The gel presented a microporous structure with pore sizes ranging from 50-350 mm. The good viability of encapsulated 7F2 osteoblasts indicated non-toxicity of the gelation conditions. In vitro release of rhBMP-2 in phosphate buffer solution showed B11% release in 360 h. The ability of the hydrogel to maintain the in vivo bioactivity of rhBMP-2 was evaluated in a bilateral critical size calvarial bone defect model in Col3.6 transgenic fluorescent reporter mice. The presence of fluorescent green osteoblast cells with overlying red alizarin complexone and yellow stain indicating osteoclast TRAP activity confirmed active cell-mediated mineralization and remodelling process at the implantation site. The complete closure of the defect site at 4 and 8 weeks post implantation demonstrated the potent osteoinductivity of the rhBMP-2 containing gel.

show abstract

Section: Resultsmentioning

confidence: 93%

Evaluation of enzymatically crosslinked injectable glycol chitosan hydrogel

et al. 2015

View full text Add to dashboard Cite

show abstract

“…A variety of choices are available for the design of dual gelling systems. In a common scheme, temperature-sensitive polymers based on PNIPAAm, PEO-PPO-PEO and poly(organophosphazene) were modified with various chemically functional groups for self-occurring post-gelation reactions including Michael additions, Schiff's base reactions, enzymemediated cross-linking, click reactions, epoxy reactions with amines or phosphates and native chemical ligation [149][150][151][152][153][154][155][156][157][158]. Photocrosslinking reactions of vinyl groups have been another solution to secondarily crosslink the thermal-sensitive hydrogels of PEGMEMA-PPGMA-EGDMA copolymers or the newly developed shearthinning dock-and-lock hydrogels of peptide-modified hyaluronic acid [159,160].…”

Section: Outlook Of Dual Gelling Hydrogelsmentioning

confidence: 99%

“…Benzaldehyde en-capped Pluronic F-127 underwent a secondary chemical crosslinking step with amine groups of glycol chitosan to yield a dual gelling system [156]. Schiff's base reaction was accelerated in high pH, and the gel thus had pH-sensitive characteristics, which were demonstrated in a sol-gel diagram.…”

Section: Outlook Of Dual Gelling Hydrogelsmentioning

confidence: 99%

Injectable polymeric hydrogels for the delivery of therapeutic agents: A review

Nguyễn

Huynh

Park

et al. 2015

European Polymer Journal

206

120

View full text Add to dashboard Cite

“…83 As the PEO-PPO-PEO has a low critical solution temperature (LCST), the hydrogel will also respond to temperature (Fig. 17).…”

Section: Schiff-base Linker Outside Polymer Main Chainmentioning

confidence: 99%

Schiff's base as a stimuli-responsive linker in polymer chemistry

2012

View full text Add to dashboard Cite

Schiff-base reactions are widely used in the field of chemistry. With many advantages, such as mild reaction conditions and high reaction rates, they were employed for protecting various functional groups and synthesizing a series of organic ligands. In polymer chemistry, they can serve as potential pH-responsive linkers in polymer chains because of their sensitive responses to changes in the pH value. With certain particular designs, the Schiff-base structure can cooperate with other reversible covalent bonds or supra-molecular interactions to form assemblies or gels, providing various functions and applications. This article aims to give a critical review of the recent literature on the Schiff-base reactions used in polymer chemistry and how they serve as a way of linking structures together. We will also cover some of the important developments on the functions and applications of these polymers.

show abstract

Dually Responsive Injectable Hydrogel Prepared by In Situ Cross-Linking of Glycol Chitosan and Benzaldehyde-Capped PEO-PPO-PEO

Cited by 128 publications

References 36 publications

Evaluation of enzymatically crosslinked injectable glycol chitosan hydrogel

Evaluation of enzymatically crosslinked injectable glycol chitosan hydrogel

Injectable polymeric hydrogels for the delivery of therapeutic agents: A review

Schiff's base as a stimuli-responsive linker in polymer chemistry

Contact Info

Product

Resources

About