Duffy blood group system and the malaria adaptation process in humans

Carvalho, Gledson Barbosa de; Carvalho, Glauber Barbosa de

doi:10.5581/1516-8484.20110016

Cited by 16 publications

(15 citation statements)

References 114 publications

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“…It is clearly obvious that Duffy-negativity provides signi cant protection against P. vivax blood-stage infection, particularly in symptomatic patients presenting for treatment, though this protection is not absolute. This is in agreement with long-prevailing thinking that for P. vivax invasions to occur an interaction between the parasites and antigens of the Duffy blood group system is necessary (46,47). However, several other host cell receptors have recently been identi ed as being involved in the parasite invasion pathway of RBCs.…”

Section: Discussionsupporting

confidence: 88%

High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana

Brown

Pappoe-Ashong

Duah

et al. 2020

Preprint

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Background The Duffy-negative phenotype is a condition which is thought to confer complete resistance against blood infection with Plasmodium vivax. However, recent studies from different malaria-endemic regions including western Africa have now shown that P. vivax can infect red blood cells (RBCs) and cause clinical disease in Duffy-negative people. The actual prevalence of P. vivax in local populations in Ghana is unknown. In addition, little information is available about the distribution of Duffy genotypes in Ghana. We determined the prevalence of P. vivax and the distribution of Duffy genotypes in Ghana. Methods DNA was extracted from dried blood spots (DBS) collected from 952 subjects (845 malaria patients and 107 asymptomatic persons) from nine locations in Ghana. Plasmodium species identification was carried out by nested polymerase chain reaction (PCR) amplification of the small-subunit rRNA genes; P. vivax was further characterized by PCR analysis of the central region of the Pvcsp gene. Duffy blood group genotyping was performed by PCR with sequence-specific primers (PCR-SSP) to detect the presence of the FYES allele. Results No cases of P. vivax were detected in any of the samples by both PCR methods used. Majority of infections (542, 94.8%) in the malaria patient samples were due to P. falciparum with only 1 infection (0.0017%) and 2 infections (0.0034%) due to P. malariae and P. ovale, respectively. No case of mixed infection was identified. Of the samples tested for the FYES allele from all the sites, 90.5% (862/952) had the FYES allele. All positive samples were genotyped as FY*B-33/FY*B-33 (Duffy-negative homozygous) and therefore classified as Fy(a-b-). Conclusions No cases of P. vivax were detected by both PCRs and 90.5% of the subjects tested carried the FYES allele. The lack of P. vivax infections observed can be attributed to the high frequency of the FYES allele that silences erythroid expression of the Duffy. These results provide insights on the host susceptibility for P. vivax infections that has before not been investigated in Ghana.

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Section: Discussionsupporting

confidence: 88%

High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana

Brown

Pappoe-Ashong

Duah

et al. 2020

Preprint

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“…The Duffy blood group locus is located on the long arm of chromosome 1 (1.q22–1.q23) and consists of three major alleles: FY*A (encoding the Fy a ), FY*B (encoding the Fy b ), and FY*B ES . The FY*A and FY*B alleles differ by a single nucleotide polymorphism (SNP) at nucleotide 125 (G125A, rs12075) resulting in the amino acid change Gly42Asp . FY*B ES whose homozygote corresponds to the Fy (a–b–) phenotype is caused by a SNP from T to C at position‐33 in the GATA box motif of the FY*B promoter.…”

Section: Introductionmentioning

confidence: 99%

A New Method for Analyzing the Duffy Blood Group Genotype by TaqMan Minor Groove Binding Probes

Zhou

Liu

An³

et al. 2014

Clinical Laboratory Analysis

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“…The Duffy (gp-FY; CD234) gene is the fourth red blood cell (RBC) gene after thalassemia, sickle cell anaemia and glucose-6-phosphate dehydrogenase (G6PD) associated with resistance to Plasmodium species [23] with particular protection against vivax malaria. Also known as the Duffy antigen receptor for chemokines (DARC), it is a variable receptor usually expressed on the surface of the red blood cell (RBC) and employed by P. vivax merozoites in gaining access in the RBCs and establishing its erythrocytic infection [24]. The DARC, located on chromosome 1 has two exons, and a single nucleotide substitution from a thymine (T) to a cytosine (C), upstream in the promoter region nullifies the expression of this gene on the RBCs, resulting in the FYO* allele [25].…”

Section: Introductionmentioning

confidence: 99%

Presence of additional P. vivax malaria in Duffy negative individuals from Southwestern Nigeria: Short Report

Oboh

Singh

Ndiaye

et al. 2020

Preprint

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Background Malaria in sub-Saharan Africa (sSA) is thought to be hugely caused by Plasmodium falciparum . Recently, growing reports of cases due to P. ovale , P. malariae , and P. vivax have been significantly reported to play a role in malaria epidemiology in sSA. This in fact is due to the usage of very sensitive diagnostic tools (e.g. PCR) which have highlighted the underestimation of non-falciparum malaria in this sub-region. P. vivax was historically thought to be absent in sSA due to the high prevalence of the Duffy null antigen in individuals residing in this sub-continent. For example, recent studies reporting the detection of vivax malaria in Duffy-negative individuals from Mali, Mauritania, Cameroon to mention a few challenges this notion.Methods Following our earlier report of P. vivax in Duffy-negative individuals, we have collected and assessed RDT and/or microscope malaria positive samples following the conventional PCR method and DNA sequencing to confirm both single/mixed infections as well as the Duffy status of the individuals.Results Amplification of Plasmodium gDNA was possible in 59.9% (145/242) of the evaluated isolates and as expected P. falciparum was the most predominant (91.7%) species identified. Interestingly, four P. vivax isolates were identified either as single (3) or mixed (1 – P. falciparum / P. vivax ) infection. Sequencing results confirmed, all vivax isolates as truly vivax malaria and their Duffy status to be that of the Duffy-negative genotype.Conclusion Identification of more vivax isolates among these Duffy-negative individuals from Nigeria, substantiate the expanding body of evidence on the ability of P. vivax to infect RBCs that do not express the DARC gene. Hence, such genetic-epidemiological study should be conducted at the national level in order to evaluate the actual burden of P. vivax in the country.

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Duffy blood group system and the malaria adaptation process in humans

Cited by 16 publications

References 114 publications

High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana

High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana

A New Method for Analyzing the Duffy Blood Group Genotype by TaqMan Minor Groove Binding Probes

Presence of additional P. vivax malaria in Duffy negative individuals from Southwestern Nigeria: Short Report

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