Duganella aceris sp. nov., isolated from tree sap and proposal to transfer of Rugamonas aquatica and Rugamonas rivuli to the genus Duganella as Duganella aquatica comb. nov., with the emended description of the genus Rugamonas

Duganella dendranthematis sp. nov. and Massilia forsythiae sp. nov., isolated from flowers

“…According to the 16S rRNA gene sequence-based phylogenetic tree, the members of the genus Duganella and Massilia were found to be polyphyletic and intermingled with the genera Janthinobacterium , Pesudoduganella and Rugamonas [4–6]. The genus Duganella , including validly and invalidly named species, were isolated from wastewater [3], a maize field [6], subtropical streams [7], sap from Acer pictum [5], zebrafish [4], ginseng soil [8], leaf surface of Trifolium repens [9] and rhizosphere of field-grown sugar cane [10]. The genus Massilia was first proposed for an isolate from a culture of blood from a febrile patient with common variable immunodeficiency [11].…”

Duganella vulcania sp. nov., Rugamonas fusca sp. nov., Rugamonas brunnea sp. nov. and Rugamonas apoptosis sp. nov., isolated from subtropical streams, and phylogenomic analyses of the genera Janthinobacterium, Duganella, Rugamonas, Pseudoduganella and Massilia

“…After 36 h of cultivation, the OD 600 was measured with a UV spectrophotometer (UV-1200, MAPADA) to determine growth in the inoculated test-tube compared with the control. Antibiotic-sensitivity tests were conducted using 6 mm diameter filter-paper discs (Hangzhou Microbial Reagent) containing the following 30 compounds (μg per disc, unless otherwise stated): penicillin (10 U per disc), oxacillin (1), ampicillin (10), carbenicillin (100), piperacillin (100), cephalexin (30), cefamezin (30), cefradine (30), cefuroxime (30), ceftazidime (30), ceftriaxone (30), cefoperazone (75), amikacin (30), gentamicin (10), kanamycin (30), neomycin (30), tetracycline (30), doxycycline (30), minocycline (30), erythromycin (15), norfloxacin (10), midecamycinum (30), ofloxacin (5), ciprofloxacin (5), vancomycin (30), polymyxin B (300 IU per disc), compound sulfamethoxazole (1.25), furazolidone (300), chloroamphenicol (30) and clindamycin (2). The discs were placed onto R2A agar plates which were spread with the strains and then incubated at 24 °C for 48 h. The inhibition zones were assessed as described previously [47].…”

“…The genus Duganella proposed by Hiraishi et al in 1997 took Zoogloea ramigera (P. R. Dugan 115) as its type species Duganella zoogloeoides [8]. Since then, 16 novel species within this genus have been reported: Duganella phyllosphaerae , Duganella ginsengisoli , Duganella sacchari , Duganella radicis , Duganella alba, Duganella aquatilis , Duganella margarita, Duganella levis , Duganella rivi , Duganella fentianensis , Duganella qianjiadongensis , Duganella lactea , Duganella guangzhouensis , Duganella flavida , ‘ Duganella aceris ’ and Duganella callida [9–16]. However, D.…”

“…Since then, only Rugamonas aquatica and Rugamonas rivuli isolated from subtropical streams in PR China [20] and Rugamonas violacea isolated from freshwater in Antarctica [21] have been reported. However, R. aquatica and R. rivuli were combined as 'Duganella aquatica' by Jeon et al recently [15]. The genus Massilia was proposed by La Scola et al (1998) based on the species Massilia timonae isolated from blood of an immunocompromised patient with cerebellar lesions [22], and was emended later by Kämpfer et al [23] and Singh et al [24].…”

“…aquatica and R. rivuli were combined as ‘ Duganella aquatica ’ by Jeon et al recently [15]. The genus Massilia was proposed by La Scola et al (1998) based on the species Massilia timonae isolated from blood of an immunocompromised patient with cerebellar lesions [22], and was emended later by Kämpfer et al [23] and Singh et al [24].…”