Dunaliella salina-derived peptide protects from bone loss: Isolation, purification and identification

Chen, Yixuan; Chen, Shiguo; Zheng, Yangfan; Yu, Huilin; Zhao, Jiawen; Cheng, Juan; Jin, Zhu

doi:10.1016/j.lwt.2020.110437

Cited by 14 publications

(12 citation statements)

References 30 publications

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“…Holistic approaches coupling network pharmacology modulation and zebrafish GIOP model as a screening platform to test the efficacy of identified matrixes were also successfully implemented. These allowed, for example, to isolate osteogenic enriched extracts derived from Erxian decoction ( Wang et al, 2019 ), osteogenic alkaloids extracts from S. acutum ( Liu et al, 2020 ), but also osteogenic peptides, polysaccharides and other anabolic compounds from microalgae ( Chen et al, 2021 ) and plants ( Zhang S. et al, 2018 ; Yan et al, 2019 ; Fu et al, 2020 ). Teleost OP models were also used to screen natural compound libraries and unveil new activities for old compounds ( Yang et al, 2019 ; Wu et al, 2020 ).…”

Section: Applicationsmentioning

confidence: 99%

Fish Models of Induced Osteoporosis

Rosa

Laizé

Gavaia

et al. 2021

Front. Cell Dev. Biol.

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Osteopenia and osteoporosis are bone disorders characterized by reduced bone mineral density (BMD), altered bone microarchitecture and increased bone fragility. Because of global aging, their incidence is rapidly increasing worldwide and novel treatments that would be more efficient at preventing disease progression and at reducing the risk of bone fractures are needed. Preclinical studies are today a major bottleneck to the collection of new data and the discovery of new drugs, since they are commonly based on rodent in vivo systems that are time consuming and expensive, or in vitro systems that do not exactly recapitulate the complexity of low BMD disorders. In this regard, teleost fish, in particular zebrafish and medaka, have recently emerged as suitable alternatives to study bone formation and mineralization and to model human bone disorders. In addition to the many technical advantages that allow faster and larger studies, the availability of several fish models that efficiently mimic human osteopenia and osteoporosis phenotypes has stimulated the interest of the academia and industry toward a better understanding of the mechanisms of pathogenesis but also toward the discovery of new bone anabolic or antiresorptive compounds. This mini review recapitulates the in vivo teleost fish systems available to study low BMD disorders and highlights their applications and the recent advances in the field.

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Section: Applicationsmentioning

confidence: 99%

Fish Models of Induced Osteoporosis

Rosa

Laizé

Gavaia

et al. 2021

Front. Cell Dev. Biol.

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“…In the last decade, microalgae peptides from Chlorella sp., Dunaliella sp., and Pavlova sp. have been investigated because of their high potential for use in pharmaceuticals due to their cancer-inhibiting or immunomodulating properties [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications

Torres-Díaz

Abreu-Takemura

Díaz-Vázquez

2022

Life

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Microalgae peptides have many medical and industrial applications due to their functional properties. However, the rapid degradation of peptides not naturally present in biological samples represents a challenge. A strategy to increase microalgae peptide stability in biological samples is to use carriers to protect the active peptide and regulate its release. This study explores the use of gold nanoparticles (AuNPs) as carriers of the Chlorella microalgae peptide (VECYGPNRPQF). The potential of these peptide biomolecules as stabilizing agents to improve the colloidal stability of AuNPs in physiological environments is also discussed. Spectroscopic (UV-VIS, DLS) and Microscopic (TEM) analyses confirmed that the employed modification method produced spherical AuNPs by an average 15 nm diameter. Successful peptide capping of AuNPs was confirmed with TEM images and FTIR spectroscopy. The stability of the microalgae peptide increased when immobilized into the AuNPs surface, as confirmed by the observed thermal shifts in DSC and high zeta-potential values in the colloidal solution. By optimizing the synthesis of AuNPs and tracking the conferred chemical properties as AuNPs were modified with the peptide via various alternative methods, the synthesis of an effective peptide-based coating system for AuNPs and drug carriers was achieved. The microalgae peptide AuNPs showed lower ecotoxicity and better viability than the regular AuNPs.

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“…12 In comparison to collagen or its hydrolysates, plant-based peptides may be better for preparing antiosteopenic calcium chelates because of the risk of allergy or other diseases associated with the collagen derived from mammals. 14,15 Walnut peptides was able to coordinate with Ca 2+ to form calciumchelating peptides that improved bone mineral density and bone formation in calcium-deficient rat. 16 Peptides from defatted lemon basil seeds can chelate calcium to form 'peptide-calcium complexes', promoting calcium absorption and transport in Caco-2 cells.…”

Section: Introductionmentioning

confidence: 99%

“…19 Previous work showed that microalgae peptides could significantly improve the bone formation marker, bone mineral density and bone microarchitecture in ovariectomized rats, indicating the great potential of microalgae to protect from bone loss in osteoporosis. 15 However, little attention has been paid to marine microalgae-based peptides, let alone the osteogenic activity of calcium-chelating microalgae peptide. 15 Therefore, this study aimed to investigate the osteogenic activities of microalgae calcium-chelating peptides obtained from four microalgae proteins: Chlorella pyrenoidosa protein (CPP), Nannochloropsis oceanica protein (NOP), Arthospira platensis protein (APP) and Dunaliella salina protein (DSP).…”

Section: Introductionmentioning

confidence: 99%

“…15 However, little attention has been paid to marine microalgae-based peptides, let alone the osteogenic activity of calcium-chelating microalgae peptide. 15 Therefore, this study aimed to investigate the osteogenic activities of microalgae calcium-chelating peptides obtained from four microalgae proteins: Chlorella pyrenoidosa protein (CPP), Nannochloropsis oceanica protein (NOP), Arthospira platensis protein (APP) and Dunaliella salina protein (DSP). The digestive properties of microalgae proteins (MPs) were investigated, and microalgae protein hydrolysates were prepared after in vitro gastrointestinal digestion.…”

Section: Introductionmentioning

confidence: 99%

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Osteogenic activities of four calcium‐chelating microalgae peptides

Chen

Jin

2022

J Sci Food Agric

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BACKGROUND: Adequate calcium intake is necessary to prevent osteoporosis, which poses significant public health challenges. The natural bioactive peptide calcium chelates have been regarded as superior calcium supplements. Microalgae peptides are regarded as potential candidates for protection from bone loss in osteoporosis. This study aimed to prepare microalgae calcium-chelating peptides from four microalgae proteins and assess their osteogenic activities in osteoporosis-like zebrafish. RESULTS: After in vitro gastrointestinal digestion, 4.42% Chlorella pyrenoidosa protein, 2.74% Nannochloropsis oceanica protein, 6.07% Arthospira platensis protein and 10.47% Dunaliella salina protein were retained. The calcium-chelating capacities of four microalgae protein hydrolysates (MPHs) ranged from 14.10 ± 7.16% to 34.11 ± 9.34%. CaCl 2 addition increased the maximum absorption peaks, absorption intensities and particle sizes of MPHs. Calcium-chelating MPHs showed stronger osteogenic activities than MPHs in the osteoporosis-like zebrafish model, with significantly increased mineralized tissue area and integrated optical density.CONCLUSION: Microalgae proteins have favorable digestibilities. Among the four MPHs, Nannochloropsis oceanica protein hydrolysates showed the highest calcium-chelating capacity, which might be due to its high degree of hydrolysis after in vitro digestion and high content of Ser, Tyr, Thr, Asp and Glu. The absorption intensities and particle sizes of MPHs both increased after calcium addition. MPH treatment could reverse dexamethasone-induced osteoporosis of zebrafish, and MPHs-Ca chelates showed higher osteogenic activities in osteoporosis-like phenotype zebrafish.

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Dunaliella salina-derived peptide protects from bone loss: Isolation, purification and identification

Cited by 14 publications

References 30 publications

Fish Models of Induced Osteoporosis

Fish Models of Induced Osteoporosis

Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications

Osteogenic activities of four calcium‐chelating microalgae peptides

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