Duplex PCR assay for detection of chickpea chlorotic dwarf virus and peanut witches’ broom phytoplasma in chickpea

Reddy, Madem Gurivi; Rao, G. P.

doi:10.1007/s13205-021-03094-y

3 Biotech

2021

DOI: 10.1007/s13205-021-03094-y

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Duplex PCR assay for detection of chickpea chlorotic dwarf virus and peanut witches’ broom phytoplasma in chickpea

Madem Gurivi Reddy

G. P. Rao

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2024

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Cited by 2 publications

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A Duplex PCR Assay for Rapid Detection of Klebsiella pneumoniae and Chryseobacterium in Large Yellow Croaker Fish

Hu,

Yin,

Luo

et al. 2024

Foodborne Pathogens and Disease

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A Duplex PCR Assay for Rapid Detection of Klebsiella pneumoniae and Chryseobacterium in Large Yellow Croaker Fish

Hu,

Yin,

Luo

et al. 2024

Foodborne Pathogens and Disease

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Evaluation of the Elimination Efficency of Apple Stem Grooving Virus by Different Detection Methods

Hu,

Dong,

Zhang

et al. 2024

THE JAPS

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Apple stem grooving virus (ASGV) frequently occurs in apple (Malus) trees and shows high sequence variability. A sensitive detection is essential for effective forecast and control of this virus. The reverse transcription-duplex polymerase chain reaction (RT-dPCR) was developed after screening of primer combinations, adjustment of annealing temperature, and optimization of dosage of primer pair combination and cDNA. Then, RT-dPCR and RT-regular PCR (RT-rPCR) were used to detect ASGV in regenerated apple plants after thermotherapy and in nature growing apple trees. The results showed that the detection efficiency of RT-dPCR was the same as the total of two RT-rPCRs. Moreover, RT-dPCR was found a sensitive, rapid and simple method to detect ASGV from various apple plants. These findings might be useful in the prediction of viral disease in host plants and can also be helpful to construct the same detection assays for other viruses. Keywords: Apple stem grooving virus; Detection efficiency; RT-duplex PCR; Sensitivity; Various apple plants; Virus detection.

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Duplex PCR assay for detection of chickpea chlorotic dwarf virus and peanut witches’ broom phytoplasma in chickpea

Cited by 2 publications

References 20 publications

A Duplex PCR Assay for Rapid Detection of Klebsiella pneumoniae and Chryseobacterium in Large Yellow Croaker Fish

A Duplex PCR Assay for Rapid Detection of Klebsiella pneumoniae and Chryseobacterium in Large Yellow Croaker Fish

Evaluation of the Elimination Efficency of Apple Stem Grooving Virus by Different Detection Methods

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