Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor

Zha, Yonghong; Li, Yansong; Hu, Pan; Lu, Shiying; Ren, Hong-Lin; Liu, Zengshan; Yang, Hualin; Zhou, Yu

doi:10.1021/acs.analchem.1c02736

Cited by 26 publications

(11 citation statements)

References 43 publications

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“…FIA uses fluorescein as a tracer to label antibodies or antigens and combines the specificity of immunological reactions with the sensitivity of fluorescence techniques. Acetochlor, metolachlor, and propisochlor were detected using this technology 20 . CLIA directly labels a luminescent substance of an excited state intermediate generated under excitation of a reactant on an antigen or an antibody and combines high-sensitivity chemiluminescence determination with high-specificity immune reaction.…”

Section: Application Of Biotechnology For Raw Materials Analysismentioning

confidence: 99%

Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses

Chen

Zhang

et al. 2022

Acta Pharmaceutica Sinica B

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Section: Application Of Biotechnology For Raw Materials Analysismentioning

confidence: 99%

Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses

Chen

Zhang

et al. 2022

Acta Pharmaceutica Sinica B

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“…Therefore, it is very important to rapidly, reliably, and quantitatively detect carbaryl. At present, the commonly used methods of detecting carbaryl include chromatography [ 9 ], spectrophotometry [ 10 ], and immunoassay [ 11 ]. These methods are sensitive and reliable.…”

Section: Introductionmentioning

confidence: 99%

A Novel Paper-Based Electrochemical Biosensor Based on N,O-Rich Covalent Organic Frameworks for Carbaryl Detection

Xiao

Wang

et al. 2022

Biosensors

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A new N,O-rich covalent organic framework (COFDHNDA-BTH) was synthesized by an amine-aldehyde condensation reaction between 2,6-dialdehyde-1,5-dihydroxynaphthalene (DHNDA) and 1,3,5-phenyltriformylhydrazine (BTH) for carbaryl detection. The free NH, OH, and C=O groups of COFDHNDA-BTH not only covalently couples with acetylcholinesterase (AChE) into the pores of COFDHNDA-BTH, but also greatly improves the catalytic activity of AChE in the constrained environment of COFDHNDA-BTH’s pore. Under the catalysis of AChE, the acetylthiocholine (ATCl) was decomposed into positively charged thiocholine (TCl), which was captured on the COFDHNDA-BTH modified electrode. The positive charges of TCl can attract anionic probe [Fe(CN)6]3−/4− on the COFDHNDA-BTH-modified electrode to show a good oxidation peak at 0.25 V (versus a saturated calomel electrode). The carbaryl detection can inhibit the activity of AChE, resulting in the decrease in the oxidation peak. Therefore, a turn-off electrochemical carbaryl biosensor based on a flexible carbon paper electrode loaded with COFDHNDA-BTH and AChE was constructed using the oxidation peak of an anionic probe [Fe(CN)6]3−/4− as the detection signal. The detection limit was 0.16 μM (S/N = 3), and the linear range was 0.48~35.0 μM. The sensor has good selectivity, repeatability, and stability, and has a good application prospect in pesticide detection.

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“…There are many kinds of immunoassays with various detection signals, such as colorimetric immunoassays, fluorescence immunoassay, chemiluminescent immunoassay, electrochemical immunoassay, electrochemiluminescence immunoassay, and so on. Typically, the detection is usually achieved by using various signal labels on the antibodies, such as radioactive isotopes [ 1 ], fluorophores [ 2 ], and enzymes [ 3 ]. Therefore, due to the high biocatalytic efficiency of enzymes, it has become a widely used label to construct enzyme-linked immunosorbent assays (ELISAs) [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Immunoassays Based on Nanozymes

et al. 2022

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As a rapid and simple method for the detection of multiple targets, immunoassay has attracted extensive attention due to the merits of high specificity and sensitivity. Notably, enzyme-linked immunosorbent assay (ELISA) is a widely used immunoassay, which can provide high detection sensitivity since the enzyme labels can promote the generation of catalytically amplified readouts. However, the natural enzyme labels usually suffer from low stability, high cost, and difficult storage. Inspired by the advantages of superior and tunable catalytic activities, easy preparation, low cost, and high stability, nanozymes have arisen to replace the natural enzymes in immunoassay; they also possess equivalent sensitivity and selectivity, as well as robustness. Up to now, various kinds of nanozymes, including mimic peroxidase, oxidase, and phosphatase, have been incorporated to construct immunosensors. Herein, the development of immunoassays based on nanozymes with various types of detection signals are highlighted and discussed in detail. Furthermore, the challenges and perspectives of the design of novel nanozymes for widespread applications are discussed.

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Duplex-Specific Nuclease-Triggered Fluorescence Immunoassay Based on Dual-Functionalized AuNP for Acetochlor, Metolachlor, and Propisochlor

Cited by 26 publications

References 43 publications

Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses

Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses

A Novel Paper-Based Electrochemical Biosensor Based on N,O-Rich Covalent Organic Frameworks for Carbaryl Detection

Recent Advances in the Immunoassays Based on Nanozymes

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