Duplicated Hox genes in the spider Cupiennius salei

Schwager, Evelyn E.; Schoppmeier, Michael; Pechmann, Matthias; Damen, Wim G.M.

doi:10.1186/1742-9994-4-10

Cited by 67 publications

(96 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These include the discovery of multiple copies of homeobox-containing genes in the spider Cupiennius salei (Schwager et al, 2007), the scorpion Centruroides sculpturatus (Sharma et al, 2014a) and the horseshoe crab Limulus polyphemus (Nossa et al, 2014). The latter paper showed by a variety of methods, including linkage mapping, comparison of age distribution of paralogous genes and gene cluster comparison, that WGD occurred in L. polyphemus.…”

Section: Introductionmentioning

confidence: 99%

Ancestral whole-genome duplication in the marine chelicerate horseshoe crabs

et al. 2015

View full text Add to dashboard Cite

Whole-genome duplication (WGD) results in new genomic resources that can be exploited by evolution for rewiring genetic regulatory networks in organisms. In metazoans, WGD occurred before the last common ancestor of vertebrates, and has been postulated as a major evolutionary force that contributed to their speciation and diversification of morphological structures. Here, we have sequenced genomes from three of the four extant species of horseshoe crabs-Carcinoscorpius rotundicauda, Limulus polyphemus and Tachypleus tridentatus. Phylogenetic and sequence analyses of their Hox and other homeobox genes, which encode crucial transcription factors and have been used as indicators of WGD in animals, strongly suggests that WGD happened before the last common ancestor of these marine chelicerates 4135 million years ago. Signatures of subfunctionalisation of paralogues of Hox genes are revealed in the appendages of two species of horseshoe crabs. Further, residual homeobox pseudogenes are observed in the three lineages. The existence of WGD in the horseshoe crabs, noted for relative morphological stasis over geological time, suggests that genomic diversity need not always be reflected phenotypically, in contrast to the suggested situation in vertebrates. This study provides evidence of ancient WGD in the ecdysozoan lineage, and reveals new opportunities for studying genomic and regulatory evolution after WGD in the Metazoa.

show abstract

Section: Introductionmentioning

confidence: 99%

Ancestral whole-genome duplication in the marine chelicerate horseshoe crabs

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Sequences of At-h and At-hh were available from GenBank. Fragments of At-Dfd, At-lab, At-dac, At-Pax6, and At-six3 were obtained via RT-PCR with degenerate primers (20,22,34) and additional sequence via RACE-PCR. Accession numbers are as follows: AB096074 (At-otd-1), AB125743 (At-h), AB125742 (At-hh), AB125741 (At-en), FM945396 (At-Dfd), FM945395 (At-lab), FM945397 (Atdac), FM945394 (At-Pax6), and FM945393 (At-six3).…”

Section: Methodsmentioning

confidence: 99%

“…S3 C and D). In wild-type embryos, At-lab is strongly expressed in the pedipalpal segment, but not in more anterior structures (19,20) (Fig. 2F; Fig.…”

mentioning

confidence: 99%

Dynamic gene expression is required for anterior regionalization in a spider

Pechmann

McGregor

Schwager

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Patterning of a multicellular embryo requires precise spatiotemporal control of gene expression during development. The gradient of the morphogen bicoid regulates anterior regionalization in the syncytial blastoderm of Drosophila. However many arthropod embryos develop from a cellular blastoderm that does not allow the formation of transcription factor gradients. Here we show that correct anterior development of the cellularized embryo of the spider Achaearanea tepidariorum requires an anterior-to-posterior wave of dynamic gene expression for positioning the stripes of hairy, hedgehog, and orthodenticle expression. Surprisingly, this dynamic repositioning of the expression of these segmentation genes is blocked in orthodenticle pRNAi embryos and no anterior structures are specified in those embryos. Our data suggest that dynamic gene expression across a field of cells is required for anterior regionalization in spiders and provides an explanation for the problem of how positional values for anterior segmentation genes are specified via a morphogen-independent mechanism across a field of cells.Achaearanea tepidariorum ͉ evolution and development ͉ hairy ͉ orthodenticle ͉ segmentation

show abstract

“…A stripe of cells expressing wg and ci is localized adjacently just anterior to a stripe of cells that express hh and en. Evidence that the boundary between these cell populations is a functional one comes from the expression of Hox genes that follows this boundary in various arthropods (Martinez-Arias and Lawrence, 1985;Lawrence, 1988;Damen, 2002;Hughes and Kaufmann, 2002c;Schwager et al, 2007). Furthermore, limb buds abut this boundary and, at least in the spider, grooves are associated with this boundary between wg and en expressing cells, identical to Drosophila (Damen, 2002).…”

Section: Conservation Of the Segment-polarity Network And The Conservmentioning

confidence: 99%

Evolutionary conservation and divergence of the segmentation process in arthropods

Damen

2007

Developmental Dynamics

154

122

View full text Add to dashboard Cite

A fundamental characteristic of the arthropod body plan is its organization in metameric units along the anterior-posterior axis. The segmental organization is laid down during early embryogenesis. Our view on arthropod segmentation is still strongly influenced by the huge amount of data available from the fruit fly Drosophila melanogaster (the Drosophila paradigm). However, the simultaneous formation of the segments in Drosophila is a derived mode of segmentation. Successive terminal addition of segments from a posteriorly localized presegmental zone is the ancestral mode of arthropod segmentation. This review focuses on the evolutionary conservation and divergence of the genetic mechanisms of segmentation within arthropods. The more downstream levels of the segmentation gene network (e.g., segment polarity genes) appear to be more conserved than the more upstream levels (gap genes, Notch/Delta signaling). Surprisingly, the basally branched arthropod groups also show similarities to mechanisms used in vertebrate somitogenesis. Furthermore, it has become clear that the activation of pair rule gene orthologs is a key step in the segmentation of all arthropods. Important findings of conserved and diverged aspects of segmentation from the last few years now allow us to draw an evolutionary scenario on how the mechanisms of segmentation could have evolved and led to the present mechanisms seen in various insect groups including dipterans like Drosophila. Developmental Dynamics 236:1379 -1391, 2007.

show abstract

Duplicated Hox genes in the spider Cupiennius salei

Cited by 67 publications

References 42 publications

Ancestral whole-genome duplication in the marine chelicerate horseshoe crabs

Ancestral whole-genome duplication in the marine chelicerate horseshoe crabs

Dynamic gene expression is required for anterior regionalization in a spider

Evolutionary conservation and divergence of the segmentation process in arthropods

Contact Info

Product

Resources

About