Duplicated insertion mutation in the microtubule-associated protein Spag5 (astrin/MAP126) and defective proliferation of immature Sertoli cells in rat hypogonadic (hgn/hgn) testes

Suzuki, Hiroyoshi; Yagi, Mio; Suzuki, Katsushi

doi:10.1530/rep.1.01104

Cited by 20 publications

(26 citation statements)

References 34 publications

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“…For double staining of proteins, oocytes and NIH/3T3 cells grown on coverslips were fixed in 4% paraformaldehyde in PBS (pH 7.4) for at least 30 min at room temperature and processed for indirect immunofluorescence microscopy as described previously. 42 The immunostained cells were mounted on glass slides and examined with a Confocal Laser-Scanning Microscope (Zeiss LSM 510 META, Germany).…”

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Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes

Yuan¹,

Li²,

Liang³

et al. 2009

Cell Cycle

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Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes

Yuan¹,

Li²,

Liang³

et al. 2009

Cell Cycle

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“…Thymuses were removed from all rats, weighed, fixed in 10% phosphatebuffered formalin, and processed for paraffin sectioning as described previously [4,9,11]. Sections (thickness, 5 µm) were mounted on poly-L-lysine coated slides, stained with hematoxylin and eosin, and examined by light microscopy as described previously [4,9,11]. Differences between normal and pet/pet rats were assessed by unpaired Student ttests, with statistical significance set at P<0.05 (Excel 2008 for Mac, Microsoft Corp, Redmond, WA, U.S.A) [4,11].…”

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“…At least 4 pet/ pet and normal males each were euthanized by ether overdoses at 0, 1, 3, 7, 12, and 21 days of age. Thymuses were removed from all rats, weighed, fixed in 10% phosphatebuffered formalin, and processed for paraffin sectioning as described previously [4,9,11]. Sections (thickness, 5 µm) were mounted on poly-L-lysine coated slides, stained with hematoxylin and eosin, and examined by light microscopy as described previously [4,9,11].…”

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confidence: 99%

“…One hour before sacrifice, rats were intraperitoneally injected with 100 mg/kg body weight 5-bromo-2-deoxyuridine (BrdU; Sigma-Aldrich, St. Louis, MO, U.S.A.). Isolated thymuses were processed for histological section as described above, and BrdU-labeled cells were detected by immunohistochemistry [9,11]. Apoptotic cells were detected with an in situ apoptosis detection kit (TaKaRa Bio, Ohtsu, Japan) [9,11].…”

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confidence: 99%

“…Isolated thymuses were processed for histological section as described above, and BrdU-labeled cells were detected by immunohistochemistry [9,11]. Apoptotic cells were detected with an in situ apoptosis detection kit (TaKaRa Bio, Ohtsu, Japan) [9,11]. All sections were counterstained with hematoxylin.…”

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Postnatal Development of Hypoplastic Thymus in Semi-Lethal Dwarf pet/pet Males

Chiba

Suzuki

Aoyama

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The petit rat (pet/pet) is a new semi-lethal dwarf mutant with anomalies in the thymus and testes, defects inherited as a single autosomal recessive trait. At birth, these pet/pet rats show low birth weight and extremely small thymuses; at 140 days of age, their thymuses show abnormal involution. In the present study, we examined early postnatal development of hypoplastic pet/pet thymuses. In addition to being hypoplastic at birth, pet/pet thymus growth was almost completely impaired during the early postnatal period. As shown by cellular incorporation of BrdU, the mitotic activity was lower in pet/pet than in normal thymuses, and terminal deoxynucleotidyl transferase dUTP nick end labeling assays showed that apoptosis occurred more often in pet/pet than in normal thymus cells during the first few days after birth. These results indicate that postnatal development of the hypoplastic pet/pet thymus is defective due to the reduced proliferation and increased apoptosis of thymic cells. The rodent thymus develops as a thymic primordium from the endoderm of the third pharyngeal pouch and from the mesenchyme of the neural crest derived from the third and fourth pharyngeal pouches [7]. Postnatally, the thymus increases in size, with expansion of the cortex and medulla, reaching a maximum size at around 10 weeks of age. Following sexual maturation, the thymus begins to shrink, and its weight and volume gradually decrease thereafter [1, 2].We have previously described pet/pet rats of the PET strain; these rats are characterized by postnatal semi-lethality, severe dwarfism from birth, testis anomalies, and markedly small thymuses (Fig. 1), all of which are controlled by a recessive mutation (pet) [4]. Newborn pet/pet rats were smaller in size, and they had very small thymuses. Relative weights (absolute weight/brain weight) of various organs were significantly lower in 140-day-old pet/pet rats than in age-matched normal rats, whereas the thymus was especially smaller in the former. Histologically, 140 day-old pet/pet thymuses contained both cortex and medulla compartments, but both were quite small, and abnormal thymic follicles were observed in the subcapsular region [4]. Because pet/pet thymuses are smaller at birth and degenerate earlier than normal thymuses, we examined the postnatal development of pet/pet thymuses in the present study.All experimental procedures and care of animals were performed in accordance with the guidelines of the Animal Care Committee of Nippon Veterinary and Life Science University. We utilized pet/pet male rats, identified by their low birth weight, and normal (+/pet or +/+) males of the PET strain; all animals were fed and maintained in our laboratory as described in a previous report [4]. At least 4 pet/ pet and normal males each were euthanized by ether overdoses at 0, 1, 3, 7, 12, and 21 days of age. Thymuses were removed from all rats, weighed, fixed in 10% phosphatebuffered formalin, and processed for paraffin sectioning as described previously [4,9,11]. Sections (thickness, ...

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Morphological characteristics of p75 neurotrophin receptor‐positive cells define a new type of glial cell in the rat dorsal root ganglia

Koike

Tanaka

Hirahara

et al. 2019

J of Comparative Neurology

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In the dorsal root ganglia (DRG), two types of glial cells (Schwann cells and satellite glial cells) have been identified based on cell morphology and expression of specific markers. In the present study, we observed unknown glial cells that were positive for p75 neurotrophin receptor (p75NTR), and therefore were immunohistochemically and ultrastructurally characterized for the first time. These cells exhibited stronger immunoreactivity against an anti‐p75NTR antibody than the DRG neurons (hereafter referred to as p75NTR++ cells). Moreover, these cells covered the glial cells surrounding proximal process of the large‐diameter DRG neurons. The proximal process is called “dendro‐axon.” The p75NTR++ cells were predominantly distributed where the first myelinating Schwann cells appear. The p75NTR++ cells were also positive for the pan‐glial cell markers S100, nestin, and Sox10, but negative for fibroblast and macrophage markers. Moreover, they were negative for a satellite glial cell marker, inwardly rectifying potassium channel Kir4.1, as well as a nonmyelinating Schwann cell marker, glial fibrillary acidic protein. In addition, their morphological features were distinct from those of the myelinating Schwann cells. To investigate the three‐dimensional ultrastructure of the p75NTR++ cells, we used array tomography combined with correlative light and electron microscopic observation. Three‐dimensional ultrastructural observation revealed that the p75NTR++ cells loosely covered glial cells around the dendro‐axons with highly ramified processes. Glial cells with these morphological features have not been reported before, indicating that the p75NTR++ glial cells are a new glial cell type in the DRG. Our results will give new insights into cell–cell relationships.

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Duplicated insertion mutation in the microtubule-associated protein Spag5 (astrin/MAP126) and defective proliferation of immature Sertoli cells in rat hypogonadic (hgn/hgn) testes

Cited by 20 publications

References 34 publications

Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes

Astrin regulates meiotic spindle organization, spindle pole tethering and cell cycle progression in mouse oocytes

Postnatal Development of Hypoplastic Thymus in Semi-Lethal Dwarf pet/pet Males

Morphological characteristics of p75 neurotrophin receptor‐positive cells define a new type of glial cell in the rat dorsal root ganglia

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