Duplication of an upstream silencer of FZP increases grain yield in rice

Bai, Xufeng; Huang, Yong; Hu, Yong; Liu, Haiyang; Zhang, Bo; Smaczniak, Cezary; Hu, Gang; Han, Zhongmin

doi:10.1038/s41477-017-0042-4

Cited by 135 publications

(109 citation statements)

References 56 publications

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“…Very recently, Bai et al . () indicated that duplication of an 18‐bp fragment about 5.3 kb upstream of FZP found in specific aus accessions could further repress FZP expression and enhance yield, suggesting post‐domestication diversification of FZP .…”

Section: Discussionmentioning

confidence: 99%

“…Either intentionally or fortuitously, ancient humans selected the Del 4-bp , causing decreased FZP expression to increase the number of secondary branches and enhance grain number and yield in domesticated rice, while maintaining normal inflorescence and spikelet formation. Very recently, Bai et al (2017) indicated that duplication of an 18-bp fragment about 5.3 kb upstream of FZP found in specific aus accessions could further repress FZP expression and enhance yield, suggesting post-domestication diversification of FZP.…”

Section: Selection Of Variation In the Cis-regulatory Region Plays Anmentioning

confidence: 99%

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Variation in the regulatory region of FZP causes increases in secondary inflorescence branching and grain yield in rice domestication

Huang

Zhao

et al. 2018

The Plant Journal

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Inflorescence branching is a key agronomic trait determining rice yield. The primary branch of the ancestral wild rice (Oryza rufipogon Griff.) bears few grains, due to minimal secondary branching. By contrast, Oryza sativa cultivars have been selected to produce large panicles with more secondary branches. Here we showed that the CONTROL OF SECONDARY BRANCH 1 (COS1) gene, which is identical to FRIZZY PANICLE (FZP), plays an important role in the key transition from few secondary branches in wild rice to more secondary branches in domesticated rice cultivars. A 4-bp tandem repeat deletion approximately 2.7 kb upstream of FZP may affect the binding activities of auxin response factors to the FZP promoter, decrease the expression level of FZP and significantly enhance the number of secondary branches and grain yield in cultivated rice. Functional analyses showed that NARROW LEAF 1 (NAL1), a trypsin-like serine and cysteine protease, interacted with FZP and promoted its degradation. Consistently, downregulating FZP expression or upregulating NAL1 expression in the commercial cultivar Zhonghua 17 increased the number of secondary branches per panicle, grain number per panicle and grain yield per plant. Our findings not only provide insights into the molecular mechanism of increasing grain number and yield during rice domestication, but also offer favorable genes for improving the grain yield of rice.

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Section: Discussionmentioning

confidence: 99%

Section: Selection Of Variation In the Cis-regulatory Region Plays Anmentioning

confidence: 99%

Variation in the regulatory region of FZP causes increases in secondary inflorescence branching and grain yield in rice domestication

Huang

Zhao

et al. 2018

The Plant Journal

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“…Overexpression of OsmiR397 led to a greater number of branches and grains per main panicle and substantially enhanced grain yield (Zhang et al, 2013b). Recent research demonstrated that repressed expression FZP could prolong the panicle branching period and increase grain yield (Bai et al, 2017). Huo et al (2017) reported that NUMBER OF GRAINS 1 (NOG1) encoding an enoyl-CoA hydratase can increase the grain yield by enhancing grain number per panicle.…”

Section: Yieldmentioning

confidence: 99%

Rice Functional Genomics Research: Past Decade and Future

et al. 2018

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Rice (Oryza sativa) is a major staple food crop for more than 3.5 billion people worldwide. Understanding the regulatory mechanisms of complex agronomic traits in rice is critical for global food security. Rice is also a model plant for genomics research of monocotyledons. Thanks to the rapid development of functional genomic technologies, over 2000 genes controlling important agronomic traits have been cloned, and their molecular biological mechanisms have also been partially characterized. Here, we briefly review the advances in rice functional genomics research during the past 10 years, including a summary of functional genomics platforms, genes and molecular regulatory networks that regulate important agronomic traits, and newly developed tools for gene identification. These achievements made in functional genomics research will greatly facilitate the development of green super rice. We also discuss future challenges and prospects of rice functional genomics research.

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“…For example, FZP knockout mutants produce extra sequential rounds of branches, but fail to bear grain (Komatsu et al ), and FZP overexpressing plants have a series of defects in spikelet structure, including extra glumes and sterile spikelets (Bai et al ). However, a moderate change in FZP expression, in FZP RNAi plants, and in a germplasm population, produced normal panicles, but with increased grain yield (Bai et al ; Huang et al ).…”

Section: Discussionmentioning

confidence: 99%

Short Panicle 3 controls panicle architecture by upregulating APO2/RFL and increasing cytokinin content in rice

Huang

Bai

Luo

et al. 2019

JIPB

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Inflorescence architecture is a major determinant of spikelet numbers per panicle, a key component of grain yield in rice. In this study, Short Panicle 3 (SP3) was identified from a short panicle 3 (sp3) mutant in which T‐DNA was inserted in the promoter of SP3, resulting in a knockdown mutation. SP3 encodes a DNA binding with one finger (Dof) transcriptional activator. Quantitative real time (qRT)‐PCR and RNA in situ hybridization assays confirmed that SP3 is preferentially expressed in the young rice inflorescence, specifically in the branch primordial regions. SP3 acts as a negative regulator of inflorescence meristem abortion by upregulating APO2/RFL. SP3 both up‐ and downregulates expression of genes involved in cytokinin biosynthesis and catabolism, respectively. Consequently, cytokinin concentrations are decreased in young sp3 panicles, thereby leading to small panicles having fewer branches and spikelets. Our findings support a model in which SP3 regulates panicle architecture by modulating cytokinin homeostasis. Potential applications to rice breeding, through gene‐editing of the SP3 promoter are assessed.

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Duplication of an upstream silencer of FZP increases grain yield in rice

Cited by 135 publications

References 56 publications

Variation in the regulatory region of FZP causes increases in secondary inflorescence branching and grain yield in rice domestication

Variation in the regulatory region of FZP causes increases in secondary inflorescence branching and grain yield in rice domestication

Rice Functional Genomics Research: Past Decade and Future

Short Panicle 3 controls panicle architecture by upregulating APO2/RFL and increasing cytokinin content in rice

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