Duplication of CaMV 35
            <i>S</i>
            Promoter Sequences Creates a Strong Enhancer for Plant Genes

Kay, R.; Chan, Amy M.; Daly, Mark; McPherson, Joan

doi:10.1126/science.236.4806.1299

Cited by 857 publications

(533 citation statements)

References 29 publications

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“…No. AF400664) of TSV, downstream of an enhanced double 35S promoter [10] into the binary vector pCAMBIA 1305.1. The TSV-CP gene sequences of all the reported isolates in NCBI databank are highly conserved and AF400664 had only 0-2 % diversity in nucleotides and 0-4 % in amino acids [1].…”

Section: Plasmid Constructs and Agrobacterium Strainmentioning

confidence: 99%

Coat protein-mediated transgenic resistance of peanut (Arachis hypogaea L.) to peanut stem necrosis disease through Agrobacterium-mediated genetic transformation

et al. 2013

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The absence of resistance genes against biotic stresses like Tobacco streak virus (TSV) within compatible peanut germplasm necessitates the deployment of genetic engineering strategy to develop transgenic resistance. Transgenic resistance in peanut (Arachis hypogaea L.) to peanut stem necrosis disease caused by TSV was obtained by transferring coat protein (CP) gene of TSV through Agrobacterium-mediated transformation of de-embryonated cotyledons and immature leaves of peanut cultivars Kadiri 6 (K6) and Kadiri 134 (K134). Integration of the transgene in T 1 , T 2 and T 3 generations were confirmed by PCR with gene-specific primers. On the basis of segregation analysis of the PCR amplicons, homozygosity was confirmed in progeny from five transgenic lines. Six transgenic plants from three different single copy transgenic lines homozygous for the transgene were selected for challenge inoculation in T 3 generations. The transgenic lines remained symptomless throughout and showed traces or no systemic accumulation of virus indicating the tolerance/resistance to the TSV infection. CP gene expression was observed in transgenic lines by RT-PCR, real-time PCR and ELISA. The findings provide an effective strategy for developing peanut with resistance to peanut stem necrosis disease.

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Section: Plasmid Constructs and Agrobacterium Strainmentioning

confidence: 99%

Coat protein-mediated transgenic resistance of peanut (Arachis hypogaea L.) to peanut stem necrosis disease through Agrobacterium-mediated genetic transformation

et al. 2013

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“…RNA was prepared by a previously published method [15] with the addition of 1 M β-mercaptoethanol in the extraction buffer. Total RNA (20 µg) was denatured in 40 % (v\v) formamide and separated on a 1.5 % (w\v) agarose gel containing formaldehyde [16].…”

Section: Preparation Of Rna and Rna Blotsmentioning

confidence: 99%

Simultaneous antisense inhibition of two starch-synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin

Lloyd

LANDSCHÜTZE²,

Kossmann

1999

Biochemical Journal

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A chimaeric antisense construct was used to reduce the activities of the two major starch-synthase isoforms in potato tubers simultaneously. A range of reductions in total starch-synthase activities were found in the resulting transgenic plants, up to a maximum of 90 % inhibition. The reduction in starch-synthase activity had a profound effect on the starch granules, which became extremely distorted in appearance compared with the control lines. Analysis of the starch indicated that the amounts produced in the tubers, and the amylose content of the starch, were not affected by the reduction in activity. In order to understand why the starch granules were distorted, amylopectin was isolated and the constituent chain lengths analysed. This indicated that the amylopectin was very different to that of the

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“…analysis RNA was isolated from the different plant organs according to the procedure described in [23]. Germinated pollen were separated from ungerminated pollen grains as described above.…”

Section: Rna Isolation Edna Libraj3: Construction and Northern Blotmentioning

confidence: 99%

Identification of a pollen‐specific sucrose transporter‐like proteinNtSUT3 from tobacco

Lemoine

Bürkle²,

Barker³

et al. 1999

FEBS Letters

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Pollen cells are symplasmieally isolated during maturation and germination. Pollen therefore needs to take up nutrients via membrane carriers. Physiological measurements on pollen indicate sucrose transport in the pollen tube. A cDNA encoding a pollen-specific sucrose transporter-like protein NtSUT3 was isolated from a tobacco pollen cDNA library. NtSUT3 expression is detected only in pollen and is restricted to late pollen development, pollen germination and pollen tube growth. Altogether these data indicate that pollen is supplied not only with glucose, but also with sucrose through a specific sucrose transporter. The respective contribution of each transport pathway may change during pollen tube growth.

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Duplication of CaMV 35 S Promoter Sequences Creates a Strong Enhancer for Plant Genes

Cited by 857 publications

References 29 publications

Coat protein-mediated transgenic resistance of peanut (Arachis hypogaea L.) to peanut stem necrosis disease through Agrobacterium-mediated genetic transformation

Coat protein-mediated transgenic resistance of peanut (Arachis hypogaea L.) to peanut stem necrosis disease through Agrobacterium-mediated genetic transformation

Simultaneous antisense inhibition of two starch-synthase isoforms in potato tubers leads to accumulation of grossly modified amylopectin

Identification of a pollen‐specific sucrose transporter‐like proteinNtSUT3 from tobacco

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