Durability assessment of hydrogel mountings for contrast-enhanced micro-CT

Hildebrand, Torben; Novak, Jan; Nogueira, Liebert Parreiras; Boccaccini, Aldo Roberto; Haugen, Håvard Jostein

doi:10.1016/j.micron.2023.103533

Micron

2023

DOI: 10.1016/j.micron.2023.103533

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Durability assessment of hydrogel mountings for contrast-enhanced micro-CT

Torben Hildebrand,

Jan Novak,

Liebert Parreiras Nogueira

et al.

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2024

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Cited by 5 publications

References 19 publications

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Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea

Boix-Lemonche,

Hildebrand,

Haugen

et al. 2024

Heliyon

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Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea

Boix-Lemonche,

Hildebrand,

Haugen

et al. 2024

Heliyon

Self Cite

View full text Add to dashboard Cite

Spatial and temporal evaluation of iodine uptake and radiodensity in meniscus tissue using contrast-enhanced micro-CT

Orellana,

Grassi,

Nuss

et al. 2024

Heliyon

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Contrast‐Enhanced Micro‐CT Imaging of Murine Mandibles: A Multi‐Method Approach for Simultaneous Hard and Soft Tissue Analysis

Hildebrand,

Humphris,

Haugen

et al. 2024

J Clinic Periodontology

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AimTo develop and evaluate a novel multi‐method micro‐computed tomography (μCT) imaging protocol for enhanced visualization of both hard and soft tissues in murine mandibles, addressing the limitations of traditional imaging techniques in dental research.Materials and MethodsWe employed a contrast‐enhanced (CE) μCT imaging technique using Lugol's iodine as a contrast agent to visualize the intricate structures of murine mandibles. The protocol involved the combination of conventional μCT imaging as well as CE‐μCT, including decalcification with EDTA, allowing for simultaneous assessment of hard and soft tissues. The method is compared with standard imaging modalities, and the ability to visualize detailed anatomical features is discussed.ResultsThe CE‐μCT imaging technique provided superior visualization of murine mandibular structures, including dental pulp, periodontal ligaments and the surrounding soft tissues, along with conventional μCT imaging of alveolar bone and teeth. This method revealed detailed anatomical features with high specificity and contrast, surpassing traditional imaging approaches.ConclusionOur findings demonstrate the potential of CE‐μCT imaging with Lugol's iodine as a powerful tool for dental research. This technique offers a comprehensive view of the murine mandible, facilitating advanced studies in tissue engineering, dental pathology and the development of dental materials.

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Durability assessment of hydrogel mountings for contrast-enhanced micro-CT

Cited by 5 publications

References 19 publications

Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea

Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea

Spatial and temporal evaluation of iodine uptake and radiodensity in meniscus tissue using contrast-enhanced micro-CT

Contrast‐Enhanced Micro‐CT Imaging of Murine Mandibles: A Multi‐Method Approach for Simultaneous Hard and Soft Tissue Analysis

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