Durability of MICP-reinforced calcareous sand in marine environments: Laboratory and field experimental study

Li, Yujie; Li, Yilong; Guo, Zhen; Xu, Qiang

doi:10.1016/j.bgtech.2023.100018

Cited by 31 publications

(4 citation statements)

References 31 publications

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“…Cylindrical crystals are elongated with parallel sides and rounded ends. Several previous studies have observed these shapes in calcite crystals during MICP processes (Wang et al 2024a ; Li et al 2023b ; Zhao et al 2024 ). We found that a combination of B2 & B11 at 14 ± 0.6 °C produced cylindrical crystals (Fig.…”

Section: Resultsmentioning

confidence: 72%

“…These bacterial species sustain precipitation, laying the foundation for developing stable and compacted cementitious matrices. Recent MICP advancements include novel bacterial strains, adaptable to diverse environments, and treatments on varied soil types, including calcareous, laterite, and clay soils, demonstrating the versatility of this technique and its relevance in addressing modern challenges (Chen et al 2023 ; Ghorbanzadeh et al 2020 ; Li et al 2023b ).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic biocementation: harnessing Comamonas and Bacillus ureolytic bacteria for enhanced sand stabilization

Rajasekar,

Zhao,

et al. 2024

World J Microbiol Biotechnol

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Biocementation, driven by ureolytic bacteria and their biochemical activities, has evolved as a powerful technology for soil stabilization, crack repair, and bioremediation. Ureolytic bacteria play a crucial role in calcium carbonate precipitation through their enzymatic activity, hydrolyzing urea to produce carbonate ions and elevate pH, thus creating favorable conditions for the precipitation of calcium carbonate. While extensive research has explored the ability of ureolytic bacteria isolated from natural environments or culture conditions, bacterial synergy is often unexplored or under-reported. In this study, we isolated bacterial strains from the local eutrophic river canal and evaluated their suitability for precipitating calcium carbonate polymorphs. We identified two distinct bacterial isolates with superior urea degradation ability (conductivity method) using partial 16 S rRNA gene sequencing. Molecular identification revealed that they belong to the Comamonas and Bacillus genera. Urea degradation analysis was performed under diverse pH (6,7 and 8) and temperature (15 °C,20 °C,25 °C and 30 °C) ranges, indicating that their ideal pH is 7 and temperature is 30 °C since 95% of the urea was degraded within 96 h. In addition, we investigated these strains individually and in combination, assessing their microbially induced carbonate precipitation (MICP) in silicate fine sand under low (14 ± 0.6 °C) and ideal temperature 30 °C conditions, aiming to optimize bio-mediated soil enhancement. Results indicated that 30 °C was the ideal temperature, and combining bacteria resulted in significant (p ≤ 0.001) superior carbonate precipitation (14–16%) and permeability (> 10− 6 m/s) in comparison to the average range of individual strains. These findings provide valuable insights into the potential of combining ureolytic bacteria for future MICP research on field applications including soil erosion mitigation, soil stabilization, ground improvement, and heavy metal remediation.

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Section: Resultsmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Synergistic biocementation: harnessing Comamonas and Bacillus ureolytic bacteria for enhanced sand stabilization

Rajasekar,

Zhao,

et al. 2024

World J Microbiol Biotechnol

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“…The authors observed that the permeability value of the mixed medium was reduced by more than 34% compared to the single culture. In another study, Li et al (2023) [16] observed that only 35.19% of the initial UCS strength of the biocemented calcareous sand sample remained after 30 days of field marine environment erosion. Jain & Das (2023) [17] found that the amount of biomineral precipitation influenced the permeability of the biocemented sandy soil.…”

Section: Introductionmentioning

confidence: 96%

Effect of Bio-Cementation with Rice Husk Ash on Permeability of Silty Sand

Sorum,

Kalita

2023

Civ Eng J

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The scarcity of competent soils in the desired locations has forced geotechnical engineers to look for soil stabilization that is sustainable and environment-friendly. In this regard, bio-cementation technology has received a lot of interest in this area because of its benefits over traditional soil stabilization techniques. The present study aims to examine the influence of the bio-cementation technique with and without Rice Husk Ash (RHA) on the permeability property of silty sand. Biocemented soil samples were prepared with various combinations of the bacterial solution (0.5, 1.0, and 1.5 optical density (OD)) and cementation solution (0.5, 1.0, and 1.5 molarity) at 0, 3, 7, 14, and 28 curing days. The RHA, an agricultural waste with good pozzolanic qualities, was added to the control soil and the biocemented soil samples at 5, 10, and 15% by weight. A falling head permeability test was employed in this study. The test results showed that the permeability of the soil decreased when the bio-cementation technique, with or without RHA, was applied. The permeability of the soil decreased with increasing BS and CS concentrations in all curing days. A greater decrease in the permeability value was seen when the RHA additive was added to the bio-cemented soil. The results of the micro-analysis tests were also in support of this reduction. Overall, the addition of RHA up to 10% with 1.0 OD BS and 1.0M CS at a 14-day curing period was noted to optimally reduce the permeability property of the soil. Doi: 10.28991/CEJ-2023-09-11-016 Full Text: PDF

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“…Previous experimental studies have found that the mechanical and hydraulic properties of the material after microbial grouting reinforcement are closely related to the production and distribution of the mineralization product calcium carbonate (Al Qabany et al, 2012;Tobler et al, 2012;Zeng et al, 2019;Li et al, 2023;Tang et al, 2023). However, there are numerous factors affecting the microbial mineralization products, including bacterial solution concentration, cementation solution concentration, calcium source type, temperature, ambient pH, grouting rate, and grouting frequency, etc (Mortensen et al, 2011;De Muynck et al, 2013;Soon et al, 2014;Amarakoon and Kawasaki, 2016;Rowshanbakht et al, 2016;Al Salloum et al, 2017;Keykha et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Numerical analysis of MICP treated sand based on bio-chemo-hydro model

Li,

Chen,

Gao

2024

Front. Bioeng. Biotechnol.

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Microbially Induced Calcite Precipitation (MICP) represents an environmentally friendly and innovative soil grouting technology. Involving intricate biochemical processes, it poses challenges for a thorough investigation of factors influencing microbial grouting effectiveness through experimentation alone. Consequently, A three-dimensional numerical model was developed to predict the permeability of bio-grouting in porous media. The numerical model is validated by comparing its results with test results available in the literature. The validated model is then used to investigate the effects of variation bacterial solution concentration, cementation solution concentration, grouting rate and grouting time on grouting effectiveness. It was founded that the remediation effect was positively correlated with the bacterial solution concentration and the number of grouting. An increased grouting rate enhanced the transport efficiency of reactants. Additionally, the concentration of cementation solution exhibited no significant effect on the reduction of calcium carbonate yield and permeability.

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Durability of MICP-reinforced calcareous sand in marine environments: Laboratory and field experimental study

Cited by 31 publications

References 31 publications

Synergistic biocementation: harnessing Comamonas and Bacillus ureolytic bacteria for enhanced sand stabilization

Synergistic biocementation: harnessing Comamonas and Bacillus ureolytic bacteria for enhanced sand stabilization

Effect of Bio-Cementation with Rice Husk Ash on Permeability of Silty Sand

Numerical analysis of MICP treated sand based on bio-chemo-hydro model

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