Durable Hydrogen Peroxide Biosensors Based on Polypyrrole-Decorated Platinum/Palladium Bimetallic Nanoparticles

Abstract

Monitoring H2O2 has profound meaning for food safety and life health. Generally, non-enzymatic H2O2 biosensors based on nanomaterials are subject to tedious preparation and short working life. In this study, a facile H2O2 biosensor was proposed for simultaneously realizing rapidity, simplicity, sensitivity, and selectivity. The in situ electro-polymerized polypyrrole functionalized with electrodeposited platinum-palladium nanoparticles (PPy-PtPd) was used as the H2O2 sensing element. The Pt7Pd4 bimetallic nanoparticles may bind PPy through an NH–metal interaction and endow glassy carbon electrodes robust electrocatalytic performance to H2O2. By parameter optimization, this H2O2 biosensor showed favorable electrochemical performance toward H2O2 reduction with a wide detection range of 2.5–8000 μM, high sensitivity (1360.83 μA·mM–1·cm–2), favorable anti-interference property, reproductivity, and outstanding endurance (> 60 days at room temperature). Importantly, the proposed biosensor worked successfully for the determination of H2O2 in three kinds of biological samples. The electrochemical biosensor using nanocomposites from electrochemistry provides a simple-make and easy-use strategy for biosensor design. In the future, PPy-PtPd NPs can be easily integrated onto high-end devices including microelectrodes and implantable and wearable biosensors for detecting H2O2 in vivo and in vitro.

Efficient Electroenzymatic Cascade Catalysis for Ortho-Selective Chlorination of Phenol

“…32 The location of IL EMB at the opening of this substrate channel makes heme become more exposed for easier access, and thus, the electron transfer is more efficient in this case. 33 Based on electrochemical impedance spectroscopy (EIS) and according to the theory of Randle and Eresheler, 34,35 the charge transfer resistances (R ct ) of the CPO/GCE and CPO-IL EMB /GCE are estimated to be 4393 Ω and 2108 Ω, respectively (Figure S11). The smaller R ct of CPO-IL EMB /GCE supports the above conclusion that the location of CPO-IL EMB at the open of the substrate channel can effectively facilitate the charge transfer of [Fe (CN) 6 ] 3-/4at the electrode.…”

Selenium-based nanomaterials for biosensing applications

“…This enzyme catalyzes the conversion of chromogenic/chemiluminescent substrates to detect biomolecules such as proteins or nucleic acids 73 . Some of these examples include the use of 20 to 60 nm ferromagnetic bismuth ferrite NPs modified into carbon paste electrodes with a limit of detection (LOD) at 7.0 10 -8 M 74 ; or the development of electro-polymerized polypyrrole functionalized with platinum-palladium NPs that offered a detection range of 2.5-8000 μM along an extremely high sensitivity 75 .…”

“…73 Some of these examples include the use of 20 to 60 nm ferromagnetic bismuth ferrite NPs modified into carbon paste electrodes with a limit of detection (LOD) at 7.0Â10 À8 M; 74 or the development of electro-polymerized polypyrrole functionalized with platinumpalladium NPs that offered a detection range of 2.5-8000 mM along an extremely high sensitivity. 75 Aside from the examples above, Se becomes advantageous for detecting peroxides due to its photoelectric and semiconductor qualities, so it may be employed for this purpose, which is why some groups have begun designing biosensing platforms with SeNPs as their primary raw material. For instance, Wang et al 76 pioneered and popularized an ecofriendly biological approach to produce crystalline monoclinic SeNPs (also referred to as m-SeNPs) utilizing the Gram-positive catalase-positive bacterium Bacillus subtilis (Fig.…”

Preparation of conductive polyaniline hydrogels co‐doped with hydrochloric acid/phytic acid and their application in Ag NPs@PA/GCE biosensor for H2O2 detection

“…At present, many scholars have developed a variety of methods to test H 2 O 2 , such as fluorescence method, [7][8][9] spectrophotometer method, 10 colorimetry, 11,12 titration, 13 electrochemical sensor, [14][15][16][17][18] and so forth. Among them, electrochemical sensors have become a research hotspot in recent years because of their simple preparation, high sensitivity, [19][20][21] and fast response.…”