Duration Control of Protein Expression <i>in Vivo</i> by Light-Mediated Reversible Activation of Translation

Ogasawara, Shinji

doi:10.1021/acschembio.6b00684

Cited by 42 publications

(44 citation statements)

References 25 publications

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“…More recently, 2-phenylazo groups were incorporated at the C2 amine of a guanosine residue and subsequently installed as the 5′-cap of a mRNA sequence through in vitro transcription, thereby allowing photoisomerization at longer wavelengths. [213] In addition to a 2-phenylazo cap ( 29 ), 2- para -methyl- ( 30 ) and 2- meta -methyl- ( 31 ) phenylazo caps were also investigated for their ability to alter affinity for elF4E through steric hindrance (Figure 30a). Phenylazo cap-modified mRNA encoding a fluorescent protein was injected into zebrafish embryos at the one-cell stage and photoisomerized to the cis form using 370 nm light.…”

Section: Optical Control Of Oligonucleotidesmentioning

confidence: 99%

“…One major limitation of this tool is the requirement for potentially damaging UV‐B light for photoswitching. More recently, 2‐phenylazo groups were incorporated at the C2 amine of a guanosine residue and subsequently installed as the 5′‐cap of a mRNA sequence through in vitro transcription, thereby allowing photoisomerization at longer wavelengths . In addition to a 2‐phenylazo cap ( 29 ), 2‐ para ‐methyl‐ ( 30 ) and 2‐ meta ‐methyl‐ ( 31 ) phenylazo caps were also investigated for their ability to alter affinity for elF4E through steric hindrance (Figure a).…”

Section: Optical Control Of Oligonucleotidesmentioning

confidence: 99%

“…In the trans form, the 2-phenylazo caps inhibit binding of elF4E and translation;h owever,upon photoisomerization (370 nm) to the cis form, elF4E can bind and initiate protein expression. b) mRNAs capped with 31 were injected into zebrafish embryos at the one-cell stage and irradiated at the 8-cell and shield stages with 370 nm or 430 nm light, respectively.After photoswitching, development of asecond midline with head structures was observed.A dapted with permission from Ref [213]…”

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confidence: 99%

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Optochemical Control of Biological Processes in Cells and Animals

et al. 2018

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Biological processes are naturally regulated with high spatial and temporal control, as is perhaps most evident in metazoan embryogenesis. Chemical tools have been extensively utilized in cell and developmental biology to investigate cellular processes, and conditional control methods have expanded applications of these technologies toward resolving complex biological questions. Light represents an excellent external trigger since it can be controlled with very high spatial and temporal precision. To this end, several optically regulated tools have been developed and applied to living systems. In this review we discuss recent developments of optochemical tools, including small molecules, peptides, proteins, and nucleic acids that can be irreversibly or reversibly controlled through light irradiation, with a focus on applications in cells and animals.

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Section: Optical Control Of Oligonucleotidesmentioning

confidence: 99%

Section: Optical Control Of Oligonucleotidesmentioning

confidence: 99%

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confidence: 99%

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Optochemical Control of Biological Processes in Cells and Animals

et al. 2018

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“…More recently, 2-phenylazo groups were incorporated at the C2 amine of a guanosine residue and subsequently installed as the 5'-cap of a mRNA sequence via in vitro transcription, allowing for photoisomerization at longer wavelengths. [213] In addition to a 2-phenylazo cap (29), 2-para-methyl- (30) and 2-metamethyl-phenylazo (31) caps were also investigated for their ability to alter affinity for elF4E via steric hindrance (Figure 30a). mRNA encoding a fluorescent protein was injected into zebrafish embryos at the one cell stage and photoisomerized to the cis form using 370 nm light.…”

Section: Reversible Optical Switching Of Oligonucleotide Functionmentioning

confidence: 99%

Optochemische Steuerung biologischer Vorgänge in Zellen und Tieren

et al. 2018

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This article is protected by copyright. All rights reserved optochemical control of biology. In contrast to excellent previous reviews that focus on the chemistry of controlling biological function with light, [1] this review focuses on manipulation of cell and animal biology using photochemical approaches. Purely optogenetic approaches, not utilizing chemical operations, have been reviewed elsewhere. [2] 2. Optical control of small molecules Small molecule probes have been essential tools to perturb and control cellular processes, thereby providing an understanding of detailed biological function. Optical control of small molecule function provides an extra layer of precision to the molecular toolbox by enabling temporal and spatial control with high resolution, as discussed in the examples below. Small molecule protein dimerizers, inhibitors, metabolites, and metal ions have all been rendered lightresponsive through the chemical installation of caging groups and have been applied to the investigation of living systems. In contrast to photocaged nucleic acids, peptides, and proteins, small molecules have the added benefits of being synthetically more accessible and capable of being readily delivered into cells and animals. 2.1. Optical activation of rapamycin induced dimerization Chemical inducers of dimerization (CIDs) are prominent tools for chemical biologists to place biological processes under conditional control. [3] The most commonly utilized CID is rapamycin, which binds to FK506 binding protein (FKBP) and the FKBP-rapamycin binding domain of mTOR (FRB), forming a ternary complex. Due to the small size of FKBP and FRB, these domains have been fused to numerous proteins and subsequent heterodimerization was induced by rapamycin. Processes that have been placed under rapamycin control include Golgi/endoplasmic reticulum association to study mitosis, [4] phosphoinositide control of endocytic trafficking, [5] and inactivation of proteins by rerouting them to the mitochondria. [6] Caged rapamycin analogs allow for placement of these processes under optical control in order to enhance temporal and spatial resolution. The photocaged rapamycin analog 1 was generated through installation of a nitrobenzyl caging group at the C-40 position (Figure 1a). [7] When applied to cells, 1 still induced FKBP-FRB dimerization, indicating that the caging group alone wasn't sufficient to abrogate protein-small molecule interaction, which is consistent with previous modifications at C-40. [8] However, work by the Hahn lab had shown that a truncated FKBP, termed iFKBP, [9] exhibited increased flexibility in the loop that interacts with the C-40 position of rapamycin. This flexibility increased contacts for interaction with 1 and resulted in a distorted binding conformation that prevented formation of the ternary complex consisting of iFKBP, FRB, and 1. This system was then applied to the optical activation of FAK (focal adhesion kinase), using an engineered iFKBP-FAK fusion which rendered the kinase inactive until UV irradiation...

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“…[28] Recently, 2-phenyldiazenyl-7methyl-guanosine has been developed and used as a photoresponsive 5'-cap in vivo to control protein expression in a time-resolved manner. [29] Upon trans-to-cis isomerization, expression of the squint protein in zebrafish embryos could be increased by a factor of 7.1, demonstrating that the position of the distal aromatic ring on the 2-phenyldiazenyl-7-methylguanosine has great impact on enzyme recognition. Although demonstrated for enzymatic interactions, the effect on oligonucleotide hybridization stays unclear to this date.…”

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confidence: 99%

Photoswitchable 2‐Phenyldiazenyl‐Purines and their Influence on DNA Hybridization

2020

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Recently, photochromic derivatives of nucleobases have drawn attention for regulating oligonucleotide hybridization with light for photopharmacological applications. The nucleobase moiety provides attractive interaction for hybridization, whereas the photochromic moiety can alter the interaction upon irradiation due to conformational changes. Herein we report the synthesis of 2-phenyldiazenyl-substituted 2'-deoxyadenosine (dA Azo) and 2'-deoxyguanosine (dG Azo) and investigate their influence in a DNA context by UV/Vis absorption, fluorescence and CD spectroscopies. For comparison, the literature-known azobenzene C-nucleoside DNAzo was used as a reference system. It could be shown that photochromic purines improve overall hybridization affinity compared to azobenzene C-nucleosides. In particular, 2'-deoxyadenosine analogue dA Azo increases melting temperatures by 7.5°C in the favored trans state with 86 % of the switching efficiency of the reference system.

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Duration Control of Protein Expression in Vivo by Light-Mediated Reversible Activation of Translation

Cited by 42 publications

References 25 publications

Optochemical Control of Biological Processes in Cells and Animals

Optochemical Control of Biological Processes in Cells and Animals

Optochemische Steuerung biologischer Vorgänge in Zellen und Tieren

Photoswitchable 2‐Phenyldiazenyl‐Purines and their Influence on DNA Hybridization

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