Dye exclusion as a means to measure wetness protection of human skin

Tate, M. L.; Grove, Gary L.; Joch, Rhonda; Laabs, John; Menard, Karen S.; Nelson, Brenda; Shepard, Kristin; Zepp, Rick; Zerweck, Charles; Zuleger, Roxanne

doi:10.1111/j.1600-0846.2007.00227.x

Cited by 5 publications

(8 citation statements)

References 2 publications

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“…30 A significantly lower value in Chroma Meter reading was measured in patients with untreated AD after 10 hours compared with that seen at time point zero, indicating a significant dye penetration caused by a disrupted outside-inside barrier. Dye penetration was significantly reduced by betamethasone and even more so by pimecrolimus treatment compared with that seen in untreated control skin.…”

Section: Tewl and Dye Penetrationmentioning

confidence: 82%

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Different effects of pimecrolimus and betamethasone on the skin barrier in patients with atopic dermatitis

Jensen

Pfeiffer²,

Witt

et al. 2009

Journal of Allergy and Clinical Immunology

107

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Section: Tewl and Dye Penetrationmentioning

confidence: 82%

“…Measurements (b*-mode) were taken 15 minutes after application (for drying of the dye) and after 10 hours. 30 Color intensity decreased according to the penetration of the dye into the skin.…”

Section: Dye Penetrationmentioning

confidence: 99%

Different effects of pimecrolimus and betamethasone on the skin barrier in patients with atopic dermatitis

Jensen

Pfeiffer²,

Witt

et al. 2009

Journal of Allergy and Clinical Immunology

107

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“…For example, it has been shown that the penetration of polyethylene glycol or sodium laurylsulfate is increased in atopic dermatitis (92). Measurements of skin penetration are often difficult to perform because the compound which penetrated into the skin must be detected by chemical analysis in tape‐stripped material, biopsies, through radioactive labelling, which can no longer be performed in humans, or by the penetration of dyes which can be traced with devices like the Chromameter ® (Minolta, Osaka, Japan) (93).…”

Section: Determination Of Skin Barrier Functionmentioning

confidence: 99%

The skin: an indispensable barrier

Proksch

Brandner

Jensen

2008

Experimental Dermatology

1,534

1,163

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Abstract:The skin forms an effective barrier between the organism and the environment preventing invasion of pathogens and fending off chemical and physical assaults, as well as the unregulated loss of water and solutes. In this review we provide an overview of several components of the physical barrier, explaining how barrier function is regulated and altered in dermatoses. The physical barrier is mainly localized in the stratum corneum (SC) and consists of protein-enriched cells (corneocytes with cornified envelope and cytoskeletal elements, as well as corneodesmosomes) and lipidenriched intercellular domains. The nucleated epidermis also contributes to the barrier through tight, gap and adherens junctions, as well as through desmosomes and cytoskeletal elements. During epidermal differentiation lipids are synthesized in the keratinocytes and extruded into the extracellular domains, where they form extracellular lipid-enriched layers. The cornified cell envelope, a tough protein ⁄ lipid polymer structure, resides below the cytoplasmic membrane on the exterior of the corneocytes. Ceramides A and B are covalently bound to cornified envelope proteins and form the backbone for the subsequent addition of free ceramides, free fatty acids and cholesterol in the SC. Filaggrin is cross-linked to the cornified envelope and aggregates keratin filaments into macrofibrils. Formation and maintenance of barrier function is influenced by cytokines, 3¢,5¢-cyclic adenosine monophosphate and calcium. Changes in epidermal differentiation and lipid composition lead to a disturbed skin barrier, which allows the entry of environmental allergens, immunological reaction and inflammation in atopic dermatitis. A disturbed skin barrier is important for the pathogenesis of contact dermatitis, ichthyosis, psoriasis and atopic dermatitis.

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“…A grande maioria dos ensaios clínicos (10)(11)(12)(13)(14)(15)(16) foi realizado nos Estados Unidos, um estudo (17) foi realizado na Bélgica e outro na França (28) .…”

Section: Métodosunclassified

“…A maioria dos ensaios clínicos foi realizada com crianças (10,12,14) e três estudos foram conduzidos com adultos e crianças (13,15,17) . Uma pesquisa (16) teve sua amostra composta de mulheres adultas e somente três ensaios clínicos (11,18,28) foram realizados com idosos estudando o problema da dermatite associada à incontinência.…”

Section: Métodosunclassified

Integridade da pele prejudicada, evidenciada por dermatite da área das fraldas: revisão integrativa

2012

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http://dx.doi.org/10.5216/ree.v14i2.14977O presente estudo teve por objetivo identificar na literatura as melhores intervenções para o tratamento e prevenção da integridade da pele prejudicada relacionada ao uso de fraldas, evidenciada por dermatite. Foi realizada busca nas bases de dados LILACS, MedLine, Cochrane e PubMed. Foram identificadas 149 publicações potencialmente elegíveis para inclusão nesta revisão, selecionando-se ao final dezenove publicações que atenderam aos critérios de inclusão e foram lidas integralmente. Identificou-se que para a prevenção do problema é indicada a manutenção da área genital, perigenital e perineal, mantendo-a limpa e seca; deve-se, ainda, aumentar a frequência das trocas de fraldas, e a aplicação de camada de pomada com óxido de zinco, evitando a maceração da área. Para o tratamento recomenda-se o uso de medicação antifúngica e corticoide nos casos de infecção secundária associada e reação na pele exacerbada. Ressalta-se a escassez de literatura sobre o problema, especialmente em idosos.

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Dye exclusion as a means to measure wetness protection of human skin

Abstract: The dye exclusion method was able to demonstrate the advantage of barrier ointments for wetness protection and also to discriminate between ointment formulations.

Cited by 5 publications

References 2 publications

Different effects of pimecrolimus and betamethasone on the skin barrier in patients with atopic dermatitis

Different effects of pimecrolimus and betamethasone on the skin barrier in patients with atopic dermatitis

The skin: an indispensable barrier

Integridade da pele prejudicada, evidenciada por dermatite da área das fraldas: revisão integrativa

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