Dye-Loaded Quatsomes Exhibiting FRET as Nanoprobes for Bioimaging

Morlà-Folch, Judit; Vargas-Nadal, Guillem; Zhao, Tinghan; Sissa, Cristina; Ardizzone, Antonio; Köber, Mariana; Uddin, Mehrun; Painelli, Anna; Veciana, Jaume; Belfield, Kevin D.; Ventosa, Nora

doi:10.1021/acsami.0c03040

Cited by 25 publications

(39 citation statements)

References 49 publications

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“…Finally, the cellular penetration capability of different types of fluorescent QSs in different cell lines has been also reported. [32][33][34][35] In this respect, introducing responsiveness to external molecular inputs within fluorescent QS nanovesicle could pave the way for the rational design of multifunctional biomimetic vesicles capable of translating the recognition of target molecules into signal transduction for biosensing applications. Motivated by the above considerations, here we anchor fluorescent cholesterol-modified DNA probes (i.e., F-miRNA probe) on dye-loaded QSs, to produce FRET-active nanovesicles responsive to clinically relevant nucleic acid targets such as microRNAs (miRNA), a class of small endogenous non-coding RNAs that can be used as diagnostic and prognostic markers in multiple human diseases.…”

Section: Introductionmentioning

confidence: 99%

“…[29,30] Their high stability, that is, also in body fluids, [31] unilamellarity and particle-to-particle homogeneity make them an attractive soft material for sensing applications. Furthermore, we recently demonstrated the possibility to confine hundreds of organic dyes in a single QS [32][33][34] and even the simultaneous loading of a Förster resonance energy transfer (FRET) dye pair in QSs yielding ultra-bright nanovesicles, whose brightness compares with the most common quantum dots, [35] enabling their use for molecular detection and imaging. Finally, the cellular penetration capability of different types of fluorescent QSs in different cell lines has been also reported.…”

Section: Introductionmentioning

confidence: 99%

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Engineering DNA‐Grafted Quatsomes as Stable Nucleic Acid‐Responsive Fluorescent Nanovesicles

Rossetti

Stella

Morlà-Folch

et al. 2021

Adv Funct Materials

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The development of artificial vesicles into responsive architectures capable of sensing the biological environment and simultaneously signaling the presence of a specific target molecule is a key challenge in a range of biomedical applications from drug delivery to diagnostic tools. Herein, the rational design of biomimetic DNA-grafted quatsome (QS) nanovesicles capable of translating the binding of a target molecule to amphiphilic DNA probes into an optical output is presented. QSs are synthetic lipid-based nanovesicles able to confine multiple organic dyes at the nanoscale, resulting in ultra-bright soft materials with attractiveness for sensing applications. Dye-loaded QS nanovesicles of different composition and surface charge are grafted with fluorescent amphiphilic nucleic acid-based probes to produce programmable FRET-active nanovesicles that operate as highly sensitive signal transducers. The photophysical properties of the DNA-grafted nanovesicles are characterized and the highly selective, ratiometric detection of clinically relevant microRNAs with sensitivity in the low nanomolar range are demonstrated. The potential applications of responsive QS nanovesicles for biosensing applications but also as functional nanodevices for targeted biomedical applications is envisaged.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineering DNA‐Grafted Quatsomes as Stable Nucleic Acid‐Responsive Fluorescent Nanovesicles

Rossetti

Stella

Morlà-Folch

et al. 2021

Adv Funct Materials

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“…Absorption spectra revealed that nanovesicles either in liquid dispersion or in gel maintain DiI and DiD molecules in the nanovesicles nanostructure since no change in the main absorption bands (Abs(DiI) = 551 nm and Abs(DiD) = 650 nm) are observed. It is worth noting that DiD is prone to form aggregates such as H-dimers, generally reported by an increase in the absorption shoulder at 600 nm [58,64]. However, spectra displayed in Figure 7b point out the lack of those aggregates or any other non-monomeric forms of DiD.…”

Section: Integrity Of Delos Nanovesicles When Gellifiedmentioning

confidence: 83%

“…The integration of dyes in similar DELOS nanovesicles dispersed in water as one of the selected media has been widely studied by N. Ventosa and collaborators [57]. Previously, vesicles with 1,1 -dioctadecyl-3,3,3 ,3tetramethyl-indocarbocyanine perchlorate (DiI) and 1,1 -dioctadecyl-3,3,3 ,3 -tetramethylindodicarbocyanine perchlorate (DiD) fluorophores were successfully prepared [58]. Importantly, DiI and DiD is a well-known Fluorescence Resonance Energy Transfer (FRET) pair [59][60][61], thus, when those dyes are nearby (typically <10 nm) they experiment high FRET efficiencies, providing information about the distance between both fluorophores.…”

Section: Integrity Of Delos Nanovesicles When Gellifiedmentioning

confidence: 99%

DELOS Nanovesicles-Based Hydrogels: An Advanced Formulation for Topical Use

et al. 2022

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Topical delivery has received great attention due to its localized drug delivery, its patient compliance, and its low risk for side effects. Recent developments have focused on studying new drug delivery systems as a strategy for addressing the challenges of current topical treatments. Here we describe the advances on an innovative drug delivery platform called DELOS nanovesicles for topical drug delivery. Previously, the production of DELOS nanovesicles demonstrated potentiality for the topical treatment of complex wounds, achieving well-tolerated liquid dispersions by this route. Here, research efforts have been focused on designing these nanocarriers with the best skin tolerability to be applied even to damaged skin, and on exploring the feasibility of adapting the colloidal dispersions to a more suitable dosage form for topical application. Accordingly, these drug delivery systems have been efficiently evolved to a hydrogel using MethocelTM K4M, presenting proper stability and rheological properties. Further, the integrity of these nanocarriers when being gellified has been confirmed by cryo-transmission electron microscopy and by Förster resonance energy transfer analysis with fluorescent-labeled DELOS nanovesicles, which is a crucial characterization not widely reported in the literature. Additionally, in vitro experiments have shown that recombinant human Epidermal Growth Factor (rhEGF) protein integrated into gellified DELOS nanovesicles exhibits an enhanced bioactivity compared to the liquid form. Therefore, these studies suggest that such a drug delivery system is maintained unaltered when hydrogellified, becoming the DELOS nanovesicles-based hydrogels, an advanced formulation for topical use.

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“…The pathways for energy transfer between fluorescent substances can be divided into radiant energy transfer and nonradiative energy transfer. Fluorescence resonance energy transfer is a nonradiative energy transfer process [10,58] . This type of energy transfer can occur between different molecules and different chromophores of the same molecule.…”

Section: Biological Applications Related To Rtcsmentioning

confidence: 99%

Reduced Thiol Compounds – Induced Biosensing, Bioimaging Analysis and Targeted Delivery

et al. 2020

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Reduced thiol compounds (RTCs), such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), are important components of many amino acids and proteins. They are also basic building blocks that make up organisms and play an important role in physiology and pathology. Therefore, based on the fundamental characteristics of RTCs in vivo, researchers have reported their use in biosensing and imaging analysis and as carriers in life sciences and health sciences. In this review, we will introduce the principles of RTCs and highlight their applications in biosensing and imaging analysis and as carriers reported from 2015 to 2020. Yingshu Guo (top left) obtained her Ph.D. degree from Qingdao University of Science and Technology in 2012. She is currently a professor at Linyi University, China, with research interests in bioanalytical chemistry and chemical biology. Xiaofei Zheng (top middle) obtained his B.S. degree from Linyi University in 2019. Then he pursued his M.S. degree under the supervision of Prof. Yingshu Guo at Linyi University. His current research is focused on bioanalytical chemistry. Xiuping Cao (top right) obtained her B.S. degree from Shandong University of Traditional Chinese Medicine in 2019. Then she pursued her M.S. degree under the supervision of Prof. Yingshu Guo at Linyi University. Her current research is focused on bioanalytical chemistry and chemical biology. Wenxin Li (bottom left) obtained her B.S. degree from Qilu Institute of Technology in 2020. Then she pursued her M.S. degree under the supervision of Prof. Yingshu Guo at Linyi University. Her current research is focused on bioanalytical chemistry and chemical biology. Di Wu (bottom middle) obtained her B.S. degree from Linyi University in 2020. Then she pursued her M.S. degree under the supervision of Prof. Yingshu Guo at Linyi University. Her current research is focused on bioanalytical chemistry. Shusheng Zhang (bottom right) obtained his Ph.D. degree from Nanjing University in 1999. He is a leading researcher at Linyi University with research interests in bioanalytical chemistry.

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Dye-Loaded Quatsomes Exhibiting FRET as Nanoprobes for Bioimaging

Cited by 25 publications

References 49 publications

Engineering DNA‐Grafted Quatsomes as Stable Nucleic Acid‐Responsive Fluorescent Nanovesicles

Engineering DNA‐Grafted Quatsomes as Stable Nucleic Acid‐Responsive Fluorescent Nanovesicles

DELOS Nanovesicles-Based Hydrogels: An Advanced Formulation for Topical Use

Reduced Thiol Compounds – Induced Biosensing, Bioimaging Analysis and Targeted Delivery

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