Dye Solutions Based on Lutein and Zeaxanthin:<i>In Vitro</i>and<i>In Vivo</i>Analysis of Ocular Toxicity Profiles

Casaroli-Marano, Ricardo P.; Sousa-Martins, Diogo; Martínez-Conesa, Eva M; Badaró, Emmerson; Nunes, Renata Portella; Lima-Filho, Acácio Alves Souza; Rodrigues, Eduardo B.; Belfort, Rubens; Maia, Maurício

doi:10.3109/02713683.2014.952831

Cited by 10 publications

(10 citation statements)

References 35 publications

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“…Mein used ferret BCO2 as a model to study the enzymatic formation of apo-carotenoids from lutein, zeaxanthin, and β-cryptoxanthin and identified both volatile and non-volatile apo-carotenoid products including 3-OH-β-ionone, 3-OH-α-ionone, β-ionone, 3-OH-α-apo-10′-carotenal, 3-OH-β-apo-10′-carotenal, and β-apo-10′-carotenal (Mein et al, 2011). Rabbits tissues were used to study the in-vitro toxicity profile of lutein and zeaxanthin-based dye solutions (Casaroli-Marano et al, 2015). They did not see any structural alterations in the neurosensory retina, RPE, or choroidal complex.…”

Section: Basic Science Of the Macular Pigment Carotenoidsmentioning

confidence: 99%

“…They did not see any structural alterations in the neurosensory retina, RPE, or choroidal complex. These xanthophyll-based dye solutions have proven to be safe and can be used to stain intraocular structures in rabbits (Casaroli-Marano et al, 2015). Rabbits have also been used to study the absorption and distribution of carotenoids in plasma, liver, and adrenal glands(Yap et al, 1997).…”

Section: Basic Science Of the Macular Pigment Carotenoidsmentioning

confidence: 99%

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Lutein, zeaxanthin, and meso-zeaxanthin: The basic and clinical science underlying carotenoid-based nutritional interventions against ocular disease

Bernstein

Vachali

et al. 2016

Progress in Retinal and Eye Research

451

419

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The human macula uniquely concentrates three carotenoids: lutein, zeaxanthin, and meso-zeaxanthin. Lutein and zeaxanthin must be obtained from dietary sources such as green leafy vegetables and orange and yellow fruits and vegetables, while meso-zeaxanthin is rarely found in diet and is believed to be formed at the macula by metabolic transformations of ingested carotenoids. Epidemiological studies and large-scale clinical trials such as AREDS2 have brought attention to the potential ocular health and functional benefits of these three xanthophyll carotenoids consumed through the diet or supplements, but the basic science and clinical research underlying recommendations for nutritional interventions against age-related macular degeneration and other eye diseases are underappreciated by clinicians and vision researchers alike. In this review article, we first examine the chemistry, biophysics, and physiology of these yellow pigments that are specifically concentrated in the macula lutea through the means of high-affinity binding proteins and specialized transport and metabolic proteins where they play important roles as short-wavelength (blue) light-absorbers and localized, efficient antioxidants in a region at high risk for light-induced oxidative stress. Next, we turn to clinical evidence supporting functional benefits of these carotenoids in normal eyes and for their potential protective actions against ocular disease from infancy to old age.

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Section: Basic Science Of the Macular Pigment Carotenoidsmentioning

confidence: 99%

Section: Basic Science Of the Macular Pigment Carotenoidsmentioning

confidence: 99%

Lutein, zeaxanthin, and meso-zeaxanthin: The basic and clinical science underlying carotenoid-based nutritional interventions against ocular disease

Bernstein

Vachali

et al. 2016

Progress in Retinal and Eye Research

451

419

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“…Specifically for Retidyne, these results suggest a nonchemical bonding to the membrane and are in agreement with previous preclinical and clinical tests that showed that this natural dye is safe to be used intraocularly. 22,24,26,28 However, the results for BB do not correlate completely with reports that showed dose-and timedependent toxicity of this dye in retinal cell lines, revealing that it is prudent to use the lowest possible concentration during the surgery. 34,35 When analyzing the behavior of Retidyne Plus while in contact with the same model, we observed a change in the isotherm of ILM model.…”

Section: Discussionmentioning

confidence: 66%

“…[19][20][21] In our previous publications, we have described the use of novel intraocular dyes in which Lutein is the primary component in cataract and vitreoretinal surgeries. [22][23][24][25][26][27][28] These L/Z-based dyes have shown efficacy in staining target membranes such as AC, ILM, and the vitreous, as well as an excellent safety profile. [22][23][24][25][26][27][28] The nature of the interaction of L/Z-based dyes with their target membranes is herein investigated using Langmuir monolayer models simulating the ILM, vitreous, AC, and epiretinal membrane (ERM).…”

mentioning

confidence: 99%

Comparing the Mode of Action of Intraocular Lutein-Based Dyes With Synthetic Dyes

Sousa-Martins

Caseli

Figueiredo

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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“…Since then, studies have continued to look at lutein and its effects on the retina. A recent study by Casaroli-Marano et al [9], in 2015, demonstrated that there was no evidence of structural alterations in the neurosensory retina, retinal pigmented epithelium (RPE), or choriocapillaris-choroidal complex caused by dye solutions based on lutein and zeaxanthin.…”

Section: Introductionmentioning

confidence: 99%

Pretreatment of RPE Cells with Lutein Can Mitigate Bevacizumab-Induced Increases in Angiogenin and bFGF

Vilà¹,

Coblentz²,

Neto³

et al. 2016

Ophthalmic Res

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Purpose: To determine whether pretreatment of retinal pigmented epithelial (RPE) cells with lutein can affect the response of cells to bevacizumab therapy. Methods: One human RPE cell line (ARPE-19) was used for all experiments. The cells were treated with lutein in different concentrations (0.01, 0.1, 1, 10, or 100 μg/ml). After 24 h, all plates were treated with bevacizumab (0.25 mg/ml). Media were harvested 24 h later for sandwich ELISA-based angiogenesis arrays. A Quantibody Human Angiogenesis Array was used in order to quantify the secretion of the following 10 proangiogenic cytokines: angiogenin, ANG2, EGF, bFGF, HB-EGF, PDGF-BB, leptin, PIGF, HGF and VEGF. Results: Treatment with bevacizumab alone led to a significant decrease in VEGF, as well as a significant increase in angiogenin and bFGF. Pretreatment with 0.1 and 1.0 μg/ml of lutein led to significant decreases in both bFGF and angiogenin following treatment with bevacizumab compared to bevacizumab treatment alone. Lutein alone did not modify the secretion of proangiogenic cytokines. Conclusions: Pretreatment of human RPE cells in culture with specific doses of lutein prior to bevacizumab treatment mitigated the increase in bFGF and angiogenin caused by bevacizumab monotherapy.

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Dye Solutions Based on Lutein and Zeaxanthin:In VitroandIn VivoAnalysis of Ocular Toxicity Profiles

Abstract: L/Z-based dye solutions demonstrated a safe profile in in vitro and in vivo models, and may be a useful tool for staining intraocular structures.

Cited by 10 publications

References 35 publications

Lutein, zeaxanthin, and meso-zeaxanthin: The basic and clinical science underlying carotenoid-based nutritional interventions against ocular disease

Lutein, zeaxanthin, and meso-zeaxanthin: The basic and clinical science underlying carotenoid-based nutritional interventions against ocular disease

Comparing the Mode of Action of Intraocular Lutein-Based Dyes With Synthetic Dyes

Pretreatment of RPE Cells with Lutein Can Mitigate Bevacizumab-Induced Increases in Angiogenin and bFGF

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