Dynactin regulates bidirectional transport of dense-core vesicles in the axon and dendrites of cultured hippocampal neurons

Kwinter, David M.; Lo, Karen Y; Mafi, Pouya; Silverman, Michael

doi:10.1016/j.neuroscience.2009.05.038

Cited by 88 publications

(89 citation statements)

References 55 publications

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“…Cells were imaged live using a wide-field fluorescent microscope (DMI 6000 B, Leica) as described previously (Kwinter et al, 2009). Axons and dendrites were initially distinguished based on morphology and confirmed retrospectively by antibody staining against MAP-2, a dendritic cytoskeletal protein.…”

Section: Methodsmentioning

confidence: 99%

Amyloid- Peptide Oligomers Disrupt Axonal Transport through an NMDA Receptor-Dependent Mechanism That Is Mediated by Glycogen Synthase Kinase 3 in Primary Cultured Hippocampal Neurons

Decker¹,

Lo²,

Unger³

et al. 2010

Journal of Neuroscience

189

175

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Section: Methodsmentioning

confidence: 99%

Amyloid- Peptide Oligomers Disrupt Axonal Transport through an NMDA Receptor-Dependent Mechanism That Is Mediated by Glycogen Synthase Kinase 3 in Primary Cultured Hippocampal Neurons

Decker¹,

Lo²,

Unger³

et al. 2010

Journal of Neuroscience

189

175

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show abstract

“…Axons and dendrites were initially distinguished based on morphology and confirmed retrospectively by antibody staining against MAP-2, a dendritic cytoskeletal protein (73).…”

Section: Live Imagingmentioning

confidence: 99%

An anti-diabetes agent protects the mouse brain from defective insulin signaling caused by Alzheimer’s disease–associated Aβ oligomers

et al. 2012

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“…Glued (Quintyne et al 1999;Kwinter et al 2008;Maier et al 2008). CC1 binds dynein directly, acting as a competitive inhibitor for the interaction between dynein and dynactin; thus, dynein cannot load its cargo (Quintyne et al 1999).…”

Section: Overexpression Of P150mentioning

confidence: 99%

“…The CC1-GFP expression plasmid, which expresses the CC1 (amino acids 217-548) of p150 Glued , was a gift from Erika Holzbaur (University of Pennsylvania, Philadelphia, PA) (Quintyne et al 1999;Kwinter et al 2008;Maier et al 2008). The siRNA directed against the dynein heavy chain (GenBank NM_001376; 59-GAGA GGAGGUUAUGUUUAAUU-39) and the siGLO transfection indicator were purchased from Thermo Scientific Dharmacon.…”

Section: Reagents Plasmids and Lentiviral Vectorsmentioning

confidence: 99%

Dynein mediates the localization and activation of mTOR in normal and human cytomegalovirus-infected cells

Clippinger

Alwine

2012

Genes Dev.

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Activation of stress signaling pathways normally leads to inhibition of the mammalian target of rapamycin complex 1 (mTORC1); however, human cytomegalovirus (HCMV) infection maintains mTORC1 activity in the presence of numerous types of stress. We previously demonstrated that HCMV infection maintains mTORC1 activity during amino acid deprivation through a Ras-related GTP-binding (Rag) protein-independent mechanism. This depends on the colocalization of mTOR and its activator, Rheb (Ras homology enriched in brain)-GTP, to a perinuclear position that corresponds to the viral cytoplasmic assembly compartment (AC). The data presented here show that the HCMV-induced, amino acid depletion-resistant perinuclear localization and activation of mTORC1 occurs as early as 8 h post-infection, prior to AC formation. We show that the molecular motor dynein is required for perinuclear localization of mTORC1 in both uninfected and HCMV-infected cells. Association between dynein and mTOR is shown by coimmunoprecipitation, and inhibition of dynein function using RNAi or the small molecule inhibitor ciliobrevin A inhibits mTORC1 activity in both uninfected and HCMV-infected cells. The data suggest that mTORC1 activation requires dynein-dependent transport to a position in the cell where it can be activated. Thus, the HCMV commandeers a cellular dynein-dependent mTORC1 activation mechanism to maintain stress-resistant mTORC1 activity during infection and to form the AC.

show abstract

Dynactin regulates bidirectional transport of dense-core vesicles in the axon and dendrites of cultured hippocampal neurons

Cited by 88 publications

References 55 publications

Amyloid- Peptide Oligomers Disrupt Axonal Transport through an NMDA Receptor-Dependent Mechanism That Is Mediated by Glycogen Synthase Kinase 3 in Primary Cultured Hippocampal Neurons

Amyloid- Peptide Oligomers Disrupt Axonal Transport through an NMDA Receptor-Dependent Mechanism That Is Mediated by Glycogen Synthase Kinase 3 in Primary Cultured Hippocampal Neurons

An anti-diabetes agent protects the mouse brain from defective insulin signaling caused by Alzheimer’s disease–associated Aβ oligomers

Dynein mediates the localization and activation of mTOR in normal and human cytomegalovirus-infected cells

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