Dynamic, 3D‐Pattern Formation Within Enzyme‐Responsive Hydrogels

Straley, Karin; Heilshorn, Sarah C.

doi:10.1002/adma.200901865

Cited by 72 publications

(67 citation statements)

References 38 publications

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“…The principal dependencies of these hydrogels are related to pH, ionic strength, temperature, light, enzyme, and magnetic field variation. [5][6][7][8][9] Polysaccharidebased magnetic hydrogel (smart hydrogel) has been used in medicine, pharmacy, biology, and water purification. [7][8][9] Additionally, some specific applications are being made in controlled drug delivery, therapeutic implants, cell cultures, and cancer cell treatment.…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7][8][9] Polysaccharidebased magnetic hydrogel (smart hydrogel) has been used in medicine, pharmacy, biology, and water purification. [7][8][9] Additionally, some specific applications are being made in controlled drug delivery, therapeutic implants, cell cultures, and cancer cell treatment. [5] After the introduction of magnetite nanoparticles in the hydrogel structure, unique properties are imparted to the resulting material.…”

Section: Introductionmentioning

confidence: 99%

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Smart Hydrogels Based on Modified Gum Arabic as a Potential Device for Magnetic Biomaterial

Paulino

Guilherme

Mattoso

et al. 2010

Macro Chemistry & Physics

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By means of a conventional cross‐linking/co‐polymerization reaction of modified gum arabic (M‐GA), acrylamide (AAm), and potassium acrylate (KAAc) in the presence of magnetite (Fe3O4) nanoparticles, a magnetic field‐sensitive M‐GA‐based hydrogel (smart hydrogel) was synthesized to investigate its potential as a magnetic biomaterial and as a intelligent hydrogel. Characterizations through FT‐IR, NMR, XRD, Mössbauer, SEM, and EDX inferred that the smart hydrogel was efficiently formed. Theoretically, the smart hydrogel obtained in this work may effectively be applied as biomaterial either on remote controlled release or tissue engineering or even in other areas of science and technology.magnified image

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Smart Hydrogels Based on Modified Gum Arabic as a Potential Device for Magnetic Biomaterial

Paulino

Guilherme

Mattoso

et al. 2010

Macro Chemistry & Physics

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“…[126] Heilshorn and colleagues developed protein polymers with tailored degradation rates to create dynamic structures emerging over time through enzymatic degradation in the bulk or on the hydrogel surface, as well as to release biomolecules with distinct spatiotemporal patterns. [127] In another study, spatial patterns of enzymatic degradation in HA hydrogels were used to enable or inhibit cell remodelling required for vascular network formation. [128] …”

Section: Spatial Patterns Of Degradabilitymentioning

confidence: 99%

Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments

Cipitria

Salmerón-Sánchez

2017

Adv Healthcare Materials

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Engineering cellular microenvironments involves biochemical factors, the extracellular matrix (ECM) and the interaction with neighbouring cells. This progress report provides a critical overview of key studies that incorporate growth factor (GF) signalling and mechanotransduction into the design of advanced microenvironments. Materials systems have been developed for surface‐bound presentation of GFs, either covalently tethered or sequestered through physico‐chemical affinity to the matrix, as an alternative to soluble GFs. Furthermore, some materials contain both GF and integrin binding regions and thereby enable synergistic signalling between the two. Mechanotransduction refers to the ability of the cells to sense physical properties of the ECM and to transduce them into biochemical signals. Various aspects of the physics of the ECM, i.e. stiffness, geometry and ligand spacing, as well as time‐dependent properties, such as matrix stiffening, degradability, viscoelasticity, surface mobility as well as spatial patterns and gradients of physical cues are discussed. To conclude, various examples illustrate the potential for cooperative signalling of growth factors and the physical properties of the microenvironment for potential applications in regenerative medicine, cancer research and drug testing.

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“…By changing no more than 3% of the amino acid sequence, the kinetics of the proteolytic degradation reaction was tuned across 2 orders of magnitude, resulting in a range of slow and fast-degrading hydrogels. Moreover, by engineering the different peptide sequences into a single composite material, control of temporal and spatial degradation patterns was achieved in response to protease activity [121]. As a proof of concept, a composite protein-engineered hydrogel containing a fast-degrading region surrounded by a slow-degrading hydrogel was created.…”

Section: Biodegradable Hydrogels For Cell Invasionmentioning

confidence: 99%

Hydrogels from Protein Engineering

Greenwood‐Goodwin

Heilshorn

2012

Biomimetic Approaches for Biomaterials Development

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Protein-engineered hydrogels build upon the natural structure and function of proteins to create well-defined, multifunctional materials by cross-linking polypeptide chains together. Individual polypeptide chains are composed of independent peptide domains built from amino acid monomers. The engineered amino acid sequence is designed to form specific and reproducible structures of the selected peptide domains. The exact amino acid sequence of the polypeptide chain can be encoded into a DNA sequence, which is transfected into a host microorganism in order to produce a monodisperse batch of polypeptide chains. Interactions between individual polypeptide chains can be engineered to form hydrated macromolecular structures with tunable mechanical strength. These interchain interactions can include both noncovalent cross-linking between neighboring peptide domains as well as covalent cross-linking between amino acid residues to form stable hydrogels.In this chapter, we discuss the development of protein-engineered hydrogels with tunable mechanical and biochemical properties for biomaterial applications. First, we discuss the fundamentals of protein structure and recombinant protein synthesis. Next, we review the diversity of peptide domains that have been utilized in protein-engineered hydrogels to date, including cell-binding domains, structural domains, and molecular recognition domains. We continue to describe the various enzymatic and synthetic chemistry strategies that have been utilized to create covalently cross-linked protein-engineered hydrogels. We conclude with an in-depth discussion of cellular interactions with protein-engineered hydrogels and of the ability to tune the biomechanics and biochemistry of the materials to direct cellular phenotype.Development of novel protein-engineered hydrogels for therapeutic applications requires regulation of specific cellular behavior through tuning of the structural, mechanical, and biochemical properties of the hydrogel. The cellular response and activation of intracellular signaling pathways for cell adhesion and migration is different between traditional two-dimensional (2D) cell culture environments and native three-dimensional (3D) cell environments, thus the use of 3D materials for Biomimetic Approaches for Biomaterials Development, First Edition. Edited by João F. Mano.

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Dynamic, 3D‐Pattern Formation Within Enzyme‐Responsive Hydrogels

Cited by 72 publications

References 38 publications

Smart Hydrogels Based on Modified Gum Arabic as a Potential Device for Magnetic Biomaterial

Smart Hydrogels Based on Modified Gum Arabic as a Potential Device for Magnetic Biomaterial

Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments

Hydrogels from Protein Engineering

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