This paper investigates the dynamic response of a composite structural system known as Profiled Steel Sheet Dry Board with Concrete infill (PSSDBC) to evaluate its vibration serviceability under human walking load. For this point, thirteen (13) PSSDBC panels in the category of Low Frequency Floor (LFF) were developed using Finite Element Method (FEM). The natural frequencies and mode shapes of the studied panels were determined based on the developed finite element models. For more realistic evaluation on dynamic response of the panels, dynamic load models representing human walking load were considered based on their Fundamental Natural Frequency (FNF), and also time and space descriptions. The peak accelerations of the panels were determined and compared to the limiting value proposed by the standard code ISO 2631-2. Effects of changing thickness of the Profiled Steel Sheet (PSS), Dry Board (DB), screw spacing, grade of concrete, damping ratio, type of support, and floor span on the dynamic responses of the PSSDBC panels were assessed. Results demonstrated that although some factors reduced dynamic response of the PSSDBC system under human walking load, low frequency PSSDBC floor system could reach high vibration levels resulting in lack of comfortableness for users. Keywords structural composite floor system, profiled steel sheet dry board, vibration serviceability, human walking load, dynamic response, human comfort.