Dynamic Aspects of Biochemistry

Baldwin, Ernest

doi:10.1097/00000441-195212000-00049

Cited by 128 publications

(39 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Of these enzymes, alpha and beta amylase are responsible for the bulk of the starch hydrolysis in the cereal endosperm. Other hydrolytic enzymes, Z, D, and R enzymes (1,3,24,25), that cleave the 1-6 alpha glucosidic linkage and other rare and anomalous linkages, provide relatively small amounts of soluble carbohydrates.…”

mentioning

confidence: 99%

Site of Origin and Extent of Activity of Amylases in Maize Germination

Dure¹

1960

Plant Physiol.

View full text Add to dashboard Cite

mentioning

confidence: 99%

Site of Origin and Extent of Activity of Amylases in Maize Germination

Dure¹

1960

Plant Physiol.

View full text Add to dashboard Cite

“…These findings, although able to account for the antiketogenic action of propionate in the intact animal and possibly also in liver tissue, do not necessarily account for such antiketogenic activity in isolated guinea-pig caecal tissue. One or two alternative explanations present themselves : either propionate is converted to pyruvate as in liver, in which case its antiketogenic activity would fall into line with current theories of antiketogenesis (see Baldwin, 1952), or it may be metabolized through some undiscovered pathway, exerting its antiketogenic effect by some other mechanism.…”

Section: Discussionmentioning

confidence: 99%

Observation on the Metabolism of Short Chain Saturated Fatty Acids by Guinea‐pig Caecal Tissue

Hagen

Robinson

1954

Aust J Exp Biol Med

View full text Add to dashboard Cite

It has been known for a long time that when fatty acids are incubated with liver or kidney slices acetoacetate is formed. In the last few years ketone body production from fatty acids in the presence of cell-free extracts of these organs has been intensively studied (see Lehninger, 1952). Recently it has been shown that, when short chain saturated fatty acids are incubated with sheep rumen tissue, ketone bodies accumulate in the medium at the expense of the acids (Pennington, 1952). In view of similarities of function of guinea-pig caecum and sheep rumen (Hagen and Robinson, 1953), we thought it would be interesting to observe whether ketone body production took place when guinea-pig caecal tissue was incubated with short chain fatty acids.This communication reports a study of the ketone body production and oxj'gen consumption when guinea-pig caecal tissue is incubated with small quantities of the sodium salts of acetic, propionic, butyric, valeric, caproie and heptoic acids. METHODS.Guinea-pigs were killed by a blow on the head. Their caeca were immediately removed and slit at either end so that the contents could be washed out with ice-cold 0-9 p.c. (w/v) sodium chloride solution. The caeca were then placed in ice-cold saline solution and cut into several pieces, which were washed and rewashed until the washings were quite clear. Finally, the tissue was chopped into fine pieces with scissors; these were dried between filter paper and 100 mg. placed into Warburg flasks, each containing 3 ml. of Krebs-Ringer phosphate buffer and having KOH and filter paper in the centre well. The fatty acid as the sodium salt at pH 7 was placed in the side arm of the flask. The flasks were shaken in a bath at 37° C. Eespiration was usually allowed to proceed for an hour before tipping in the acid. This procedure was adopted in the first experiments when it was thought that a quantitative correlation might be made between oxygen uptake and acetoacetate production and that the initial oxygen consumption without substrate might serve as a measure of actively metabolizing tissue in each flask; later the procedure was continued in order that subsequent experiments might be compared with earlier ones. Shaking was continued for n further two hours after adding the substrate and oxygen consumption was measured at 15-minute intervals throughout this period.After incubation, the flask contents were emptied into centrifuge tubes, each flask washed with 3 ml. water and the washings added to the tubes. After centrifugation, 4 ml. of the supernatant fluid were used for ketone body determination by the method of Greenberg and Austral.

show abstract

“…It has been suggested that pantothenic acid is involved in purine metabolism besides being an essential growth factor for organisms which ferment carbohydrates (Baldwin, 1948).…”

Section: Discussionmentioning

confidence: 99%

Polysaccharidases of Group A Streptococci

Crowley

1951

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARY:Proteinase produced by Group A streptococci was associated with a decrease in hyaluronidase production which was not related to any other known environmental condition. The concentration of proteinase which was correlated with decreased hyaluronidase production was constant for a given strain. Inactive polysaccharidase components in cell-free filtrates were converted to active form by a number of different agents, namely, sodium sulphite, zinc and calcium ions, autoclaved broth and pantothenic acid. Bacterial dextran, pectin and inulin were depolymerized by cell-free filtrates and by partially purified enzymes derived from Type 4, Type 22 and Type 2 strains. ' Depolymerase ' was associated with hyaluronidase and amylase in biologically active material prepared by fractional precipitation.

show abstract

Dynamic Aspects of Biochemistry

Cited by 128 publications

References 0 publications

Site of Origin and Extent of Activity of Amylases in Maize Germination

Site of Origin and Extent of Activity of Amylases in Maize Germination

Observation on the Metabolism of Short Chain Saturated Fatty Acids by Guinea‐pig Caecal Tissue

Polysaccharidases of Group A Streptococci

Contact Info

Product

Resources

About