Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform

Jia, Guangyao; Ma, Kuo-Sheng; Kim, Ji-Tae; Zoval, Jim; Peytavi, Régis; Bergeron, Michel G.; Madou, Marc

doi:10.1016/j.snb.2005.04.043

Cited by 40 publications

(35 citation statements)

References 35 publications

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“…A number of studies have shown the possibility of being able to modify the exposed metal layer using readily available office printers [7,10,76] or similar techniques [73] or chemically to form self-assemble monolayers [32] of for the addition for functional groups [33]. Beyond the scope of this review, reports have shown the possibility of utilising CDs and DVDs for both sample preparation as well as quantification using approaches such as surface plasma resonance [89], centrifugal microfluidics or so called 'lab-on-a-disc' devices [90][91][92][93][94][95], DNA microarrays [96]. Their application in the exploitation of solar energy [97] and the development of batteries [98] has also been reported.…”

Section: Discussionmentioning

confidence: 99%

Cheap and disposable gold and silver electrodes: Trends in the application of compact discs and digital versatile discs for electroanalytical chemistry

Honeychurch

2017

TrAC Trends in Analytical Chemistry

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Highlights Compact discs and digital versatile discs can be readily used to give cheap reproducible Au and Ag electrodes. Their fabrication and use in electroanalytical chemistry is reviewed. Applications, performance characteristics and construction details are reviewed. AbstractIncreasingly more reports have focused on the use of digital versatile discs (DVDs) and compact discs (CDs) for the fabrication of electrodes. The majority of discs use Al to reflect the laser. However, a notable percentage utilise Au or Ag. This layer can be mechanically or chemically exposed allowing for the economic fabrication of 2 otherwise expensive Ag and Au electrodes. Cleaning steps are not required due to the layer's mirror like surface. Mass production of these discs means they are available, reproducible and disposable. Modifications can be made by laser, inkjet printing, etching or xurography. Self-assembled monolayers and the attachment of functional groups can also be made. This review (98 references and 5 figures) is divided into four sections. The first describes the development of these electrodes and their fabrication and modification. The next three sections focused on applications for the determination of metal ions, biomedical and environmental analysis.

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Section: Discussionmentioning

confidence: 99%

Cheap and disposable gold and silver electrodes: Trends in the application of compact discs and digital versatile discs for electroanalytical chemistry

Honeychurch

2017

TrAC Trends in Analytical Chemistry

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“…A servo motor and corresponding motor controller (Pacific Scientific, IL, USA) provided the rotational power (centrifugal pumping mechanism) for fluidic propulsion within the microfluidic disc. 32 The fluidic pumping apparatus (servo motor), temperature control apparatus, and the solenoidbased motion system were controlled using LABVIEW automation software (National Instruments, TX, USA). Experimental parameters for fluidic pumping and thermocycling (e.g., rotational speed, ramp rates/cooling rates, holding period for annealing/denaturing) were controlled through a LABVIEW user interface.…”

Section: B Experimental Apparatusmentioning

confidence: 99%

Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification

et al. 2013

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A comprehensive study involving numerical analysis and experimental validation of temperature transients within a microchamber was performed for thermocycling operation in an integrated centrifugal microfluidic platform for polymerase chain reaction (PCR) amplification. Controlled heating and cooling of biological samples are essential processes in many sample preparation and detection steps for micrototal analysis systems. Specifically, the PCR process relies on highly controllable and uniform heating of nucleic acid samples for successful and efficient amplification. In these miniaturized systems, the heating process is often performed more rapidly, making the temperature control more difficult, and adding complexity to the integrated hardware system. To gain further insight into the complex temperature profiles within the PCR microchamber, numerical simulations using computational fluid dynamics and computational heat transfer were performed. The designed integrated centrifugal microfluidics platform utilizes thermoelectrics for ice-valving and thermocycling for PCR amplification. Embedded micro-thermocouples were used to record the static and dynamic thermal responses in the experiments. The data collected was subsequently used for computational validation of the numerical predictions for the system response during thermocycling, and these simulations were found to be in agreement with the experimental data to within $97%. When thermal contact resistance values were incorporated in the simulations, the numerical predictions were found to be in agreement with the experimental data to within $99.9%. This in-depth numerical modeling and experimental validation of a complex single-sided heating platform provide insights into hardware and system design for multi-layered polymer microfluidic systems. In addition, the biological capability along with the practical feasibility of the integrated system is demonstrated by successfully performing PCR amplification of a Group B Streptococcus gene. V C 2013 American Institute of Physics. [http://dx

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“…Serial capillary valves in centrifugal microfluidic platforms have been demonstrated, most commonly in hydrophobic environments, with considerable success (Badr et al 2002;Jia et al 2006;Lai et al 2004;Madou et al 2006;Peytavi et al 2005). In hydrophilic environments, however, capillary valves are more difficult to control and characterize (viz., determination of burst frequency) and are more prone to change over time.…”

Section: Serial Valving On Centrifugal Microfluidic Platformsmentioning

confidence: 99%

Serial siphon valving for centrifugal microfluidic platforms

Siegrist

Gorkin

Clime

et al. 2009

Microfluid Nanofluid

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130

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Today, the focus in microfluidic platforms for diagnostics is on the integration of several analysis steps toward sample-to-answer systems. One of the main challenges to integration is the requirement for serial valving to allow the sequential release of fluids in a temporally and spatially controlled manner. The advantages offered by centrifugal microfluidic platforms make them excellent candidates for integration of biological analysis steps, yet they are limited by the lack of robust serial valving technologies. This is especially true for the majority of centrifugal microfluidic devices that rely on hydrophilic surfaces, where few passive serial valving techniques function reliably. Building on the useful functionality of centrifugal microfluidic siphoning previously shown, a novel serial siphon valve is introduced that relies on multiple, inline siphons to provide for a better controlled, sequential release of fluids. The introduction of this novel concept is followed by an analytical analysis of the device.Proof-of-concept is also demonstrated, and examples are provided to illustrate the range of functionality of the serial siphon valve. The serial siphon is shown to be robust and reproducible, with variability caused by the dependence on contact angle, rotation velocity, and fluidic properties (viz., surface tension) significantly reduced compared to current microfluidic, centrifugal serial valving technologies.

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Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform

Cited by 40 publications

References 35 publications

Cheap and disposable gold and silver electrodes: Trends in the application of compact discs and digital versatile discs for electroanalytical chemistry

Cheap and disposable gold and silver electrodes: Trends in the application of compact discs and digital versatile discs for electroanalytical chemistry

Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification

Serial siphon valving for centrifugal microfluidic platforms

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