Dynamic behaviour of selected PET tracers in embryonated chicken eggs

Gebhardt, Peter; Würbach, Lydia; Heidrich, Alexander; Heinrich, Liesa; Walther, M.; Opfermann, Thomas; Sorensen, B.; Saluz, Hans‐Peter

doi:10.1016/j.remnie.2013.09.013

Cited by 4 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine whether the chick CAM is a viable alternative to simple murine models for preclinical imaging applications, we compared the uptake and retention of the gold standard clinical radiotracer 18 F-FDG in ovo and in vivo. We found a similar 18 F-FDG uptake profile in the chick embryo compared to previous studies [33]. Due to the growth demands at this stage of development, glucose metabolism in many tissues is increased, and as a consequnce we saw high uptake thoughout the embryo, rather than in discrete organs as is typically seen in both mice and humans.…”

Section: Discussionsupporting

confidence: 84%

The Chicken Chorioallantoic Membrane as a Low-Cost, High-Throughput Model for Cancer Imaging

Smith,

Greenwood,

Tyrrell

et al. 2023

Preprint

View full text Add to dashboard Cite

Purpose: Mouse models are invaluable tools for radiotracer development and validation. They are, however, expensive, low throughput, and are constrained by animal welfare considerations. Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging studies.Methods: Growth of NCI-H460 Fluc tumors on the CAM was optimized using a range of physical and chemical supports. Tumor-bearing eggs were imaged by dynamic18F-2-fluoro-2-deoxy-D-glucose (18F-FDG) or (4S)-4-(3-18F-fluoropropyl)-L-glutamate (18F-FSPG) PET/CT following intravenous injection, with mice bearing subcutaneous NCI-H460 Fluc xenografts imaged with18F-FDG for comparison. The dependence of the transporter system xc-onin ovo18F-FSPG tumor uptake was determined through treatment with imidazole ketone erastin. Additionally,18F-FSPG PET/CT was used to monitor treatment responsein ovo24 h following external beam radiotherapy.Results: NCI-H460 Fluc cells grown in Matrigel formed vascularized tumors of reproducible size without compromising embryo viability. By designing a simple method for cannulation it was possible to perform dynamic PET imagingin ovo, producing high tumor-to-background signal for both18F-FDG and18F-FSPG.18F-FDG tumor uptake kinetics were similarin ovoand invivo, with18F-FSPG providing an early marker of both treatment response and target inhibition in CAM-grown tumors.Conclusions: The CAM provides a low-cost alternative to tumor xenograft mouse models which may broaden access to PET and SPECT imaging. Rapid tumor growth and high-quality PET images that can be obtained with this model suggest its potential use for early radiotracer screening, pharmacological studies, and monitoring response to therapy.

show abstract

Section: Discussionsupporting

confidence: 84%

The Chicken Chorioallantoic Membrane as a Low-Cost, High-Throughput Model for Cancer Imaging

Smith,

Greenwood,

Tyrrell

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The data confirmed our previous results on low toxicity (Steinemann et al, 2017) and high tolerability (H€ opfner et al, 2018) of the novel hybrid compound. Based on the initial mouse experiments, additional in vivo studies were performed employing an advanced CAM assay in which the growth of TGCT plaques and their metabolic activity was assessed by MR/PET imaging, a technique that is well established for the clinical assessment of tumor development and metabolic tumor activity in patients (Gebhardt et al, 2013;Zuo et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Antitumor and antiangiogenic activity of the novel chimeric inhibitor animacroxam in testicular germ cell cancer

et al. 2019

View full text Add to dashboard Cite

Chimeric inhibitors, which merge two drug pharmacophores in a single molecule have become a prominent approach for the design of novel anticancer compounds. Here, we examined animacroxam, which combines histone deacetylase (HDAC) inhibitory and cytoskeleton‐interfering pharmacophores, in testicular germ cell tumors (TGCT). The effectiveness of animacroxam was compared to that of the commonly applied chemotherapeutic cisplatin as well as the clinically approved HDAC inhibitor vorinostat. The antineoplastic and antiangiogenic effects of animacroxam on TGCT in vivo were assessed through exploratory animal studies and a modified chorioallantoic membrane assay, revealing that animacroxam has significant antitumor activity in TGCT. A novel positron emission tomography/MR‐imaging approach was applied to determine tumor volume and glucose [2‐fluoro‐2‐deoxy‐d‐glucose (18F‐FDG)] uptake in TGCT tumors, revealing reduced glucose uptake in animacroxam‐treated TGCTs and showing a dose‐dependent suppression of glycolytic enzymes, which led to a breakdown in glycolytic energy production. Furthermore, the observed antiangiogenic effects of animacroxam were related to its ability to inhibit endothelial cell–cell communication, as the expression of gap junction‐forming connexin 43 was strongly suppressed, and gap‐junctional intercellular mass transport was reduced. Our data suggest that the chimeric HDAC inhibitor animacroxam may become a promising candidate for the treatment of solid cancers and may serve as an interesting alternative to platinum‐based therapies.

show abstract

“…Toxic side effects and only poor correlation between narcosis depth and dose limited the application of UC and Avertin and the authors clearly favored the use of Isoflurane due to its high tolerability enabling repeated imaging of the avian embryos at a daily basis. In 2013, Gebhardt et al [70] applied a similar approach as Würbach et al [71] to initially evaluate the dynamic behavior of new PET tracers in the chick embryo model as an in vivo assay. Various 18 F, 64 Cu, and 68 Ga-labeled compounds were investigated and the potential of the chick embryo model as efficient in vivo model could be shown.…”

Section: Positron Emission Tomography (Pet)mentioning

confidence: 99%

“…With the recent advances in magnetic resonance tomography (MRI) its application to in ovo imaging has rendered feasible and many applications of the technique in the live embryo as well as after sacrification have been reported for different scientific fields, including embryonal development [47][48][49][50][51][52][53][54], ophthalmology [48][49][50][51][52][53][54][55][56][57], oncology [58][59][60][61][62][63], metabolic assessment [54,64], and initial testing of the biodistribution of new compounds [65][66][67]. Further, nuclear imaging methods have been translated to in ovo imaging, mainly for initial testing of new labeling strategies [19,[68][69][70][71].…”

Section: Introductionmentioning

confidence: 99%

In vivo PET/MRI Imaging of the Chorioallantoic Membrane

et al. 2020

View full text Add to dashboard Cite

The Hen's Egg Test Chorioallantoic Membrane (HET-CAM) of fertilized chick eggs represents a unique model for biomedical research. With its steadily increasing use, non-invasive in ovo imaging for longitudinal direct quantification of the biodistribution of compounds or monitoring of surrogate markers has been introduced. The full range of imaging methods has been applied to the HET-CAM model. From the current perspective, Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET) appear promising techniques, providing detailed anatomical and functional information (MRI) and excellent sensitivity (PET). Especially by combining both techniques, the required sensitivity and anatomical localization of the signal source renders feasible. In the following, a review of recent applications of MRI and PET for in ovo imaging with a special focus on techniques for imaging xenotransplanted tumors on the CAM will be provided.

show abstract

Dynamic behaviour of selected PET tracers in embryonated chicken eggs

Cited by 4 publications

References 19 publications

The Chicken Chorioallantoic Membrane as a Low-Cost, High-Throughput Model for Cancer Imaging

The Chicken Chorioallantoic Membrane as a Low-Cost, High-Throughput Model for Cancer Imaging

Antitumor and antiangiogenic activity of the novel chimeric inhibitor animacroxam in testicular germ cell cancer

In vivo PET/MRI Imaging of the Chorioallantoic Membrane

Contact Info

Product

Resources

About