Dynamic BMP signaling mediates notochord segmentation in zebrafish

Peskin, Brianna; Norman, Jim C.; Bagwell, Jennifer; Lin, Adam Yuh; Adhyapok, Priyom; Talia, Stefano Di; Bagnat, Michel

doi:10.1016/j.cub.2023.05.039

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…NC was enriched for matrisome proteins already described in the IVD context in human or other animal models such as COL2A1 , COL11A2 , ACAN , HAPLN3 , PRELP , SPP1 , FN1 , SLIT2 , SHH , BMP3 , LGALS3 , SEMA3C , CD109 , TIMP3 . 25 , 56 , 61 , 63 , 66 , 67 , 68 , 69 , 72 , 90 , 91 , 92 , 93 , 94 Interestingly, the analysis highlighted SEMA3C , SULF2 , HSPG2 and P4HA1 proteins, not yet described in this developmental context. They have been involved in mediating Hedgehog signaling or in post-translational ECM proteins modifications.…”

Section: Resultsmentioning

confidence: 84%

In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells

Warin,

Vedrenne,

Tam

et al. 2024

iScience

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Section: Resultsmentioning

confidence: 84%

In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells

Warin,

Vedrenne,

Tam

et al. 2024

iScience

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“…Moving beyond the classical osteoblasts, we also here provide means to investigate non-classical osteoblasts, a cell type confined to teleosts that has recently been shown to play crucial roles in the initiation of fish spine development (Grotmol et al, 2003; Lleras Forero et al, 2018; Renn et al, 2013). Very little is known to date about the mechanisms of gene regulation in these non-classical osteoblasts, limited to RNA-seq data that indicate a role for BMP and Notch signalling in patterning the cells of the notochord sheath (Wopat et al, 2018; Peskin et al, 2023). However, the gene regulatory interactions that are required to bring about the segmental expression of entpd5a remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…How this segmental expression pattern of entpd5a is established is not understood, but it is distinct from the one involved in somitogenesis (Lleras Forero et al, 2018). The role of the BMP and Notch signalling pathways has been highlighted in the segmenting notochord (Peskin et al, 2023; Wopat et al, 2018), but the transcriptional events governing entpd5a expression in classical osteoblasts and in the NSCs remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Integration of ATAC and RNA-sequencing identifies chromatin and transcriptomic signatures in classical and non-classical zebrafish osteoblasts and indicates mechanisms ofentpd5aregulation

Petratou,

Stehling,

Müller

et al. 2024

Preprint

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Two types of osteoblasts are required to assemble the zebrafish embryonic skeleton: classical osteoblasts homologous to the mammalian cell, and notochord sheath cells, which serve as non-classical osteoblasts. The geneentpd5ais critically required for ossification via both types of osteoblasts. Despite the indispensability of zebrafish models in vertebrate research, the genetic regulation of bone formation, as well as mechanisms of transcriptional control ofentpd5a, remain largely unknown. Here, using a newly generated transgenic line, we isolate classical and non-classical osteoblasts from zebrafish embryos and performed both ATAC-seq and RNA-seq. We analysed results independently and integratively to understand those chromatin dynamics and accompanying transcriptomic changes that occur in different skeletal cell types. We show that although Dlx family factors are playing important roles in classical osteoblast regulation, Hox family factors are involved in governing spinal ossification via non-classical osteoblasts. We further present a resource-driven analysis of theentpd5apromoter, experimentally validating the ATAC-seq dataset and proposing mechanisms of regulating the complexentpd5aexpression pattern during zebrafish osteogenesis. Our results thus provide a necessary comprehensive resource for the field of bone development and indicate spatio-temporally regulated promoter/enhancer interactions taking place in theentpd5alocus.

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“…Notably, while BMPs engage the same Smads across diverse cell types, the functional diversity of BMPs is, in part, attributed to distinct transcription factors [92]. These transcription factors are recruited by Smads to orchestrate the regulation of specific subsets of target genes, a process contingent upon the cellular context, as shown in Figure 2 [93]. Within this group of transcriptional regulators, Hox proteins emerge as noteworthy constituents.…”

Section: Bone Morphogenetic Proteins (Bmps)mentioning

confidence: 99%

Advancements in Genetic Marker Exploration for Livestock Vertebral Traits with a Focus on China

Khan,

Chen,

Huang

et al. 2024

Animals

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In livestock breeding, the number of vertebrae has gained significant attention due to its impact on carcass quality and quantity. Variations in vertebral traits have been observed across different animal species and breeds, with a strong correlation to growth and meat production. Furthermore, vertebral traits are classified as quantitative characteristics. Molecular marker techniques, such as marker-assisted selection (MAS), have emerged as efficient tools to identify genetic markers associated with vertebral traits. In the current review, we highlight some key potential genes and their polymorphisms that play pivotal roles in controlling vertebral traits (development, length, and number) in various livestock species, including pigs, donkeys, and sheep. Specific genetic variants within these genes have been linked to vertebral development, number, and length, offering valuable insights into the genetic mechanisms governing vertebral traits. This knowledge has significant implications for selective breeding strategies to enhance structural characteristics and meat quantity and quality in livestock, ultimately improving the efficiency and quality of the animal husbandry industry.

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Dynamic BMP signaling mediates notochord segmentation in zebrafish

Cited by 6 publications

References 29 publications

In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells

In vitro and in vivo models define a molecular signature reference for human embryonic notochordal cells

Integration of ATAC and RNA-sequencing identifies chromatin and transcriptomic signatures in classical and non-classical zebrafish osteoblasts and indicates mechanisms ofentpd5aregulation

Advancements in Genetic Marker Exploration for Livestock Vertebral Traits with a Focus on China

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