Dynamic changes in circulating and antigen‐responsive T‐cell subpopulations post‐<i>Mycobacterium bovis</i> infection in cattle

Pollock, J.M.; Pollock, D.A.; Campbell, Douglas L.; Girvin, R. Martyn; Crockard, A.D.; Neill, S.D.; Mackie, D.P.

doi:10.1046/j.1365-2567.1996.457538.x

Cited by 108 publications

(80 citation statements)

References 45 publications

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“…TNF-␣ levels from bulk lymphocytes did not correlate with depletion of WC-1 ϩ ␥/␦ T cells and were largely undetectable in macrophagedepleted splenocytes. In cattle, ␥/␦ T cells have been shown to decrease initially after infection and then increase in the peripheral circulation [34]. The data presented suggest that the pivotal role in infection for this population may arise in the transition from the acute to acquired immune response, around 21 days post infection.…”

Section: Discussionmentioning

confidence: 80%

Role of CD8+ and WC-1+ γ/δ T cells in resistance to Mycobacterium bovis infection in the SCID-bo mouse

Smith

Kreeger

Alvarez

et al. 1999

Journal of Leukocyte Biology

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The role of various effector T cell populations in the bovine immune response toMycobacterium bovis infection is poorly understood. This is largely due to the difficulties associated with performing in vivo challenge studies in the natural host species. In this report, we utilized a fetal bovine-severe combined immunodeficient (SCID-bo) xenochimeric mouse model to study the protective role of two putative effector cell types, CD8 ؉ T cells and a subpopulation of ␥/␦ T cells that express WC-1, a member of the cysteine-rich scavenger receptor superfamily (CRSR). We demonstrate that CD8 ؉ T cells play a key role in protection and contribute substantially to bovine IFN-␥ mRNA levels at 30 days post-infection. The role of WC-1 bearing cells to protection was less definitive but our results suggest that this population may play a pivotal role early in infection. Granuloma architecture was altered in anti-WC-1 (ILA29) but not anti-CD8 (ILA51) -treated animals, suggesting that this population may be involved in recruitment of various cell types to sites of infection. J. Leukoc. Biol. 65: 28-34; 1999.

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Section: Discussionmentioning

confidence: 80%

Role of CD8+ and WC-1+ γ/δ T cells in resistance to Mycobacterium bovis infection in the SCID-bo mouse

Smith

Kreeger

Alvarez

et al. 1999

Journal of Leukocyte Biology

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“…Flow cytometry of the PBMCs was performed using FACScan (EPICS ELITE ESP Coulter crop, Florida, U.S.A.) as previously described [21]. The subpopulations of lymphocytes and monocytes were identified on the basis of forward and orthogonal light scatter and were gated appropriately.…”

Section: Methodsmentioning

confidence: 99%

“…DISCUSSION CD4 + T cells are important for resistance to infection with S. Typhimurium, though the antigen specificity of this T cell response is unknown [23,[25][26][27], and there has been much reported evidence showing their roles as anti-cytozoic bacteria [20-22, 25, 28, 29] by secreting INF-γ and IL-2 from helper T (Th)-1 cells in CD4 + T cells [21,23,27]. In cases of salmonellosis, it has been reported that the number of CD4 + T cells in peripheral blood increases and that the ratio of CD4 + T cells to CD8 + T cells is increased [21,25,27]. On the other hand, in experimental oral infection in mice with S. Typhimurium, it was found that the number of γδ-T cells increases in association with the immune response [11,13].…”

Section: Facs Analysis Of Pbmcsmentioning

confidence: 99%

Inflammatory Cytokines and Antigen-Responsive Mononuclear Cells in Peripheral Blood of Cattle Infected with Salmonella Takoradi.

Konnai

Htsuka

Sakamoto³

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. To determine the immunological response in lactating dairy cows infected with Salmonella (S.) Takoradi, the relationships among distributions of peripheral blood mononuclear cell (PBMC) subpopulations, endotoxin concentrations and dynamics of inflammatory cytokines in blood were investigated. The ratio of CD4 + T cells to CD8 + T cells was significantly lower in the affected cattle than in the control cattle (p<0.05) to decrease in the number of CD4 + T cells in the infected cattle. In contrast, the numbers of γδ-T cells, MHC class II-positive cells were significantly higher in the affected cattle than in the control cattle (p<0.01 respectively). Endotoxemia was found in all but one of the affected cattle. Serum IL-1 and IL-6 bioactivities were significantly higher in the affected cattle than in the control cattle (IL-1, p<0.05; IL-6, p<0.01). Serum TNF-α activities and levels were not detected in the control and affected cattle. The activities of proinflammatory cytokines determined by the bioassay are important to the relationships between concentration of endotoxin, cytokines and clinical signs, such as leukocytosis, leukopenia, fever or bacterial shedding. Serum IL-2 levels were lower in the affected cattle than in the control cattle. Serum IFN-γ was not detected in the affected cattle except one. These results by the ELISA seemed to reflect the condition of subpopulation in the PBMCs from the shedding cattle. The present results suggest that cellular immunity is suppressed while the humoral immunity is activated in acute bovine salmonellosis. KEY WORDS: bovine salmonellosis, cytokine, endotoxin, PBMC.

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“…Datos experimentales y clínicos indican que el IFN-γ es esencial para la defensa del huésped contra M. tuberculosis, por lo que los defectos en la producción de IFN-γ se consideran un factor de riesgo para la infección por M. tuberculosis y la progresión de la enfermedad en humanos (7) . En bovinos infectados experimentalmente con M. bovis, se observó que la principal población celular productora de IFN-γ son los linfocitos T CD4 + de memoria, aunque los linfocitos T CD8 + también han sido identificados como productores importantes de esta citocina (8) . Las citocinas proinflamatorias IL-12, IL-18, IL-23 producidas por los macrófagos inducen la activación de los linfocitos T, estimulando así la síntesis de IFN-γ, el cual a su vez activa a los macrófagos para producir TNF-α.…”

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“…La importancia de las poblaciones de macrófagos y linfocitos T en la tuberculosis bovina se ha estudiado principalmente en ganado infectado experimentalmente (8) , por lo que el objetivo de este estudio fue identificar la capacidad de los macrófagos en el control de la infección por M. bovis y la producción de IFN-γ de los linfocitos en bovinos naturalmente infectados con este patógeno.…”

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Mycobacterium bovis induce una respuesta inmune celular heterogénea en bovinos infectados naturalmente

López¹,

Flores-Villalva

Pabello³

2017

Rev. Mex. Cienc. Pecu.

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la hipótesis de que las limitaciones en el funcionamiento de los macrófagos y los linfocitos T desempeñan un papel muy importante en la diseminación de la enfermedad.Palabras clave: IFN-γ, Macrófagos, Mycobacterium bovis, PPDB, Coctel proteico. Abstract:Macrophages are considered one of the main immune mechanisms of resistance to mycobacterial infections. In addition, experimental and clinical data indicate that IFN-γ is essential for host defence against M. tuberculosis. The aim of this study was to identify macrophage capacity on controlling Mycobacterium bovis infection and lymphocyte IFN-γ production from cattle naturally exposed to M. bovis. Peripheral blood monocyte-derive macrophages from naturally infected cattle were evaluated for their ability to control the intracellular growth of M. bovis BCG. Besides, whole blood cells were stimulated with PPDB and ESAT-6+CFP-10 protein cocktail. Results show a disparity in host susceptibility to Mycobacterium bovis. A M. bovis BCG survival rate of 80 to 150 % in macrophages from naturally infected cattle was observed. While the IFN-γ production towards Purified protein derivative bovine (PPDB) and the protein cocktail was 2.5 and 2.0 optic density index, respectively. Though our data do not support a correlation between the host susceptibility to M. bovis and the production of IFN-γ in naturally infected cattle, the hypothesis that the limitations in the functioning of macrophages and T lymphocytes play a very important role in the spread of the disease cannot be discarded.

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Dynamic changes in circulating and antigen‐responsive T‐cell subpopulations post‐Mycobacterium bovis infection in cattle

Cited by 108 publications

References 45 publications

Role of CD8+ and WC-1+ γ/δ T cells in resistance to Mycobacterium bovis infection in the SCID-bo mouse

Role of CD8+ and WC-1+ γ/δ T cells in resistance to Mycobacterium bovis infection in the SCID-bo mouse

Inflammatory Cytokines and Antigen-Responsive Mononuclear Cells in Peripheral Blood of Cattle Infected with Salmonella Takoradi.

Mycobacterium bovis induce una respuesta inmune celular heterogénea en bovinos infectados naturalmente

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