Dynamic Changes in Endogenous Hormones in Terminal Buds from Different Crown Positions in Sequoia sempervirens (Lamb.) Endl

Abstract

Endogenous hormones can improve plant resistance and regulate growth and development. To obtain the basis of chemical control technology for improving Sequoia sempervirens resistance in Xuzhou, China, the current study probed the dynamic changes of endogenous hormones in terminal buds from different crown positions in S. sempervirens. Enzyme-linked immunosorbent assay (ELISA) was used to measure changes in the contents of endogenous hormones in terminal buds from the upper, middle, and lower lateral branches. The results were as follows: Indole acetic acid (IAA) in all terminal positions had a similar change trend of “rise–drop–rise.” Gibberellic acid (GA) in the upper and middle terminal buds showed similar trends of “drop–rise,” but GA in the lower lateral branches presented a “rise–drop” trend. Zeatin–riboside (ZR) in all terminal positions had similar change trends of “drop–rise.” Abscisic acid (ABA) in all terminal positions had similar change trends of “drop–rise–drop.” the trend of (IAA + GA + ZR)/ABA in all terminal positions was the same as that of IAA. Our results confirmed that, in autumn, the high content and ratio of stimulatory endogenous hormones in the terminal bud of S. sempervirens induced the terminal bud cells to continue to divide and grow, and the new branches could not be fully lignified and deeply dormant before the onset of low temperatures in winter, which led to a decrease in cold resistance and even the death of the terminal buds.

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

“…Researchers have developed a variety of methods for the detection of phytohormones. Enzyme-linked immunosorbent assay (ELISA) and other immune assays are based on the ligand binding of the antibody to detect phytohormones with high sensitivity and specificity [33][34][35][36][37]. However, the specific recognition involved in immunoassays means that it is difficult to measure multiple types of hormones at the same time.…”