Dynamic changes in the accumulation of metabolites in brackish water clam Corbicula japonica associated with alternation of salinity

Koyama, Hiroki; Okamoto, Seiji; Watanabe, Naoki; Hoshino, Naoshige; Jimbo, Mitsuru; Yasumoto, Ko; Watabe, Shugo

doi:10.1016/j.cbpb.2014.11.007

Cited by 26 publications

(19 citation statements)

References 24 publications

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“…However, concentrations of other principal osmolytes, such as glycine and proline, did not vary after the water exchange. Under substantial osmotic imbalance, concomitant increases or decreases in these metabolites would also be expected (Carregosa et al 2014 ;Koyama et al 2015 ). These results suggest that the water exchange process has negligible lasting impacts on larval physiology and performance.…”

Section: Aq3mentioning

confidence: 72%

“…Metabolites reflect the final products of gene expression, but they are also tightly regulated by external environmental influences and thus more closely represent the phenotype of an organism (Patti et al 2012 ). As the molecules of metabolism, differential metabolite profiles can provide important insights into how an organism actually responds metabolically to different situations, such as temperature changes (Dunphy et al 2015 ), variations in salinity (Koyama et al 2015 ), exposure to poor water quality (Ji et al 2015 ), dietary manipulation (Jin et al 2015 ), longterm handling stress (Karakach et al 2009 ), and super-intensive culture conditions (Schock et al 2013 ), among others. Use of metabolomicsbased approaches in aquaculture research is starting to gain tract (reviewed by Alfaro and Young in review; Young and Alfaro in review).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic profiling of mussel larvae: effect of handling and culture conditions

Young

Villas‐Bôas

2015

Aquacult Int

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Gas chromatography mass spectroscopy was applied to characterize the metabolic profiles of hatchery-reared mussel (Perna canaliculus) larvae before and after a prolonged handling and water exchange process, and to investigate the effect of culture conditions. A decrease in succinate and an increase in alanine were observed after the water exchange, which indicated alterations in energy production and osmotic balance. However, these variations were subtle and it is unlikely that the water exchange practice had any lasting negative effects on larval physiology and performance. Multivariate pattern recognition tools (hierarchical clustering, principal component analysis and projection to latent squares discriminant analysis) were used to assess metabolite variations in larvae reared in low-density static and high-density flow through systems and to construct a culture condition classification model. Twelve metabolites 1

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Section: Aq3mentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of mussel larvae: effect of handling and culture conditions

Young

Villas‐Bôas

2015

Aquacult Int

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“…See Koyama et al . () for an applied example of how CE‐MS was used to gain detailed metabolic insights into salinity adaption of brackish‐water clams from four commercial fishery grounds in Japan with different water chemistries.…”

Section: Metabolomic Strategiesmentioning

confidence: 99%

Metabolomic strategies for aquaculture research: a primer

Young

Alfaro

2016

Reviews in Aquaculture

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Metabolomics is a fast-evolving field that provides qualitative and quantitative analyses of metabolites within cells, tissues or biofluids. Recent applications of metabolomics approaches in aquaculture research have highlighted the huge potential for solving problems within all aspects of the production line, from hatchery production to postharvest quality control. To assist with the growing application of metabolomics in aquaculture research, this contribution provides a review of techniques and steps necessary to conduct metabolomics research, from experimental design to data interpretation. Specifically, we target scientists who are new to the field of metabolomics, and we offer simple, but comprehensive steps and strategies to conduct this type of research. We conclude this primer with some advice on how to access relevant expertise and facilities for metabolomics-based aquaculture research.

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“…A similar CE‐MS approach has been applied to gill and foot muscle of brackish water clam, collected from different water areas. No metabolite is allowing distinguishing between male and female specimens, but b‐alanine, choline, GABA, ornithine, and glycine betaine levels vary with the water salinity . Finally, CE associated with GC‐MS and microarray experiments have been applied to evaluate the impact of postharvest storage on Citrus grandis flavor.…”

Section: Ce‐ms As a Metabolomic Toolmentioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

et al. 2015

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We describe the most important research articles published on amino acid analysis using CE during the period from June 2013 to May 2015, and follows the format of the previous articles published in electrophoresis the new developments in amino acid analysis with CE are mainly describing improvements in detection means and injection methods. Enantiomeric separation developments are still important. Focusing the applications, we describe the neurochemical and clinical works, but also the metabolomic studies for which the publication number increase greatly. Finally, works focused on amino acids in food and agricultural applications are described.

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Dynamic changes in the accumulation of metabolites in brackish water clam Corbicula japonica associated with alternation of salinity

Cited by 26 publications

References 24 publications

Metabolic profiling of mussel larvae: effect of handling and culture conditions

Metabolic profiling of mussel larvae: effect of handling and culture conditions

Metabolomic strategies for aquaculture research: a primer

Recent advances in amino acid analysis by capillary electromigration methods, 2013–2015

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