Dynamic changes in the transcriptome of Populus hopeiensis in response to abscisic acid

Abstract

Abscisic acid (ABA) plays a fundamental role in plant response and adaptation to abiotic stresses, such as drought, high salinity and low temperature. Populus hopeiensis exhibits exceptional tolerance to water-deficit environments and is therefore an excellent choice for studying drought tolerance in trees. This study provides a global view of transcriptome dynamics in P. hopeiensis in response to exogenous ABA using Illumina RNA-sequencing. Endogenous ABA content increased and reached a peak at 8 h after ABA treatment and then significantly decreased at latter time points. Differential expression analysis and Gene ontology enrichment revealed that the number of transcripts exhibited significant increase during the first 8 hours after ABA treatment, which then significantly decreased at 12 and 24 h. Transcription factors (TFs) analysis showed that six different patterns were observed based on the expression of the six TFs families (AP2/ERF, NAC, MYB, MYB-related, bZIP and WRKY) and the majority of differentially expressed TFs increased rapidly after ABA treatment. This study provides a robust resource for investigating the functions of genes induced by ABA and will help to develop a better understanding of the molecular regulatory mechanism in response to drought in poplar.

Comparative transcriptome analysis of shoot and root tissue of Bacopa monnieri identifies potential genes related to triterpenoid saponin biosynthesis

“…The real-time expression analysis of these transcription factors in response to MeJA and wounding has further identified NAC and bHLH transcription factor showing the better response in comparison with other families such as WRKY and AP2/ERF protein. Similarly, dynamic changes in the expression pattern were observed in different transcription factor families in Populus hopeiensis after ABA treatment [ 41 ]. Also, the transcriptome based tissue-specific expression of transcription factor genes provides important information for understanding the development and transcriptional regulation of the paper mulberry [ 42 ].…”

Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance in Populus davidiana

“…Transcriptional responses subsequently dictate large-scale response pathways allocating resources and optimizing plant performance under stress through multiple routes. These include biological pathways regulating abiotic stress responses through the regulation of developmental phytohormones such as IAA, ABA, ethylene, GA, and defense hormones SA and JA (Chen et al, 2017). In this study, we found several genes involved in these hormonal pathways with changes in the expression by at least 2-folds following the stress treatment.…”

Transcriptome analysis identifies genes involved in the somatic embryogenesis of Eucalyptus

“…Among them, one ABA receptor and two ABA 8'-hydroxylase genes were speci cally down-regulated in the dedifferentiated tissue of E. camaldulensis. Chen et al, reported that the ABA transcripts peaked at 8h after ABA treatment and then signi cantly decreased at latter time points [33]. Although we lack multiple time points of the callus development, it can be speculated that the down-regulation of ABA genes might be necessary for the callus development of Eucalyptus.…”