Dynamic changes of bacterial community under the influence of bacterial-feeding nematodes grazing in prometryne contaminated soil

Zhou, Jihai; Sun, Xiangwu; Jiao, Jiaguo; Liu, Manqiang; Hu, Feng; Li, Huixin

doi:10.1016/j.apsoil.2012.11.005

Cited by 23 publications

(9 citation statements)

References 46 publications

(60 reference statements)

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“…The DNA and proteins that are mixed in with the organic matter are not easily separated by DGGE or SDS-PAGE. In addition, relatively small populations of microbes and low abundances of proteins may not be identified by DGGE and SDS-PAGE, respectively [8,38]. In this study, for example, both the DGGE and SDS-PAGE profiles have a dark background and smeared dark areas at the bottom of SDS-PAGE gel, which make the accuracy and reliability of the analysis questionable.…”

Section: Isrn Soil Sciencementioning

confidence: 86%

“…We also detected an increased relative abundance in the microbial populations with increasing distance from the 40 55 25 35 15 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 smelter, demonstrating that heavy metals exerted a major effect on bacterial diversity by promoting changes in species composition (represented by the position of the bands) and in species richness (represented by the number of occurring bands). Namely, contamination inhibited certain bacterial groups and stimulated others, and it changed the overall microbial diversity [2,3,38,43,44]. Subsequently, the content and composition of the soil proteins were studied to investigate their changes under heavy metals stress.…”

Section: Discussionmentioning

confidence: 99%

“…investigate soil microbial diversity was first introduced by Muyzer et al (1993). DGGE proved to be capable of accurately measuring soil microbial diversity, so it has become the predominant technique in soil microbial ecology for profiling soil microbial communities and monitoring shifts in microbial community composition due to environmental changes [38]. The DGGE profiles of the soil bacteria and actinomycetes are shown in Figures 3(a) and 4(a).…”

Section: Microbial Biomass Carbon In Soilmentioning

confidence: 99%

“…Although PCR-DGGE and SDS-PAGE are useful for monitoring the changes in microbial community structures, they still have drawbacks. These approaches are easily interfered with by impurities that are coextracted with the soil DNA and proteins, primarily humic substances [7,38]. The DNA and proteins that are mixed in with the organic matter are not easily separated by DGGE or SDS-PAGE.…”

Section: Isrn Soil Sciencementioning

confidence: 99%

See 3 more Smart Citations

The Variations in the Soil Enzyme Activity, Protein Expression, Microbial Biomass, and Community Structure of Soil Contaminated by Heavy Metals

Zhang

Liu

et al. 2013

ISRN Soil Science

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Heavy metals have adverse effects on soil ecology. Given the toxicity of heavy metals, there is an urgent need to select an appropriate indicator that will aid in monitoring their biological effects on soil ecosystems. By combining different monitoring techniques for various aspects of microbiology, the effects of heavy metals on soil microorganisms near a smelter were studied. Our goal was to determine whether proteins could be a proper indicator for soil pollution. This study demonstrated that the activities of acid phosphatase and dehydrogenase, as well as the levels of microbial biomass carbon and proteins, were negatively affected by heavy metals. In addition, significantly negative correlations were observed between these microbial indicators and heavy metals. Denaturing gradient gel electrophoresis analysis was used in this study to demonstrate that heavy metals also have a significantly negative effect on soil microbial diversity and community structure. The soil protein expression was similar across different soils, but a large quantity of presumably low molecular weight protein was observed only in contaminated soil. Based on this research, we determined that the soil protein concentration was more sensitive to heavy metals than acid phosphatase, dehydrogenase, or microbial biomass carbon because it was more dramatically decreased in the contaminated soils. Therefore, we concluded that the soil protein level has great potential to be a sensitive indicator of soil contamination. Further research is essential, particularly to identify the low molecular weight protein that only appears in contaminated soil, so that further insight can be gained into the responses of microbes to heavy metals.

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Section: Isrn Soil Sciencementioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Microbial Biomass Carbon In Soilmentioning

confidence: 99%

Section: Isrn Soil Sciencementioning

confidence: 99%

See 2 more Smart Citations

The Variations in the Soil Enzyme Activity, Protein Expression, Microbial Biomass, and Community Structure of Soil Contaminated by Heavy Metals

Zhang

Liu

et al. 2013

ISRN Soil Science

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“…[ 27 ] Nematodes’ grazing upon bacteria and fungi has been reported to enhance the overall growth of bacteria and fungi. [ 28 ] Nematodes are also considered as key soil regulators for decomposition that further link with other microorganisms through various interactions providing a base for the soil–food web. Therefore, microbial composition and diversity play a significant role to maintain soil ecosystem via complex interactions.…”

Section: Introductionmentioning

confidence: 99%

The Fate of Lead (Pb) in Multitrophic Interactions Among Bacteria, Fungi, and Bacterivorous Soil Nematodes

Iqbal

Ahmed

Pervez

et al. 2020

CLEAN Soil Air Water

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Contamination of potentially toxic metals poses threats to living organisms due to prolonged existence in an ecosystem. The effects of toxic metals on soil microbes have broadly been reported but the study on interactions among soil micro‐ and macro‐organisms under metal stress needs to be elaborated. In current study, the immobilization of lead (Pb) is investigated by using five resistant microbial strains; (2) Bacillus cereus, (1) Bacillus subtilis, (1) Brevundimonas naejangsanensis, and (1) fungal strain Fusarium equiseti isolated from contaminated soil of Hattar, Pakistan. Bacterial and fungal strains and their consortia are tested in the presence/absence of nematodes for Pb immobilization in a medium containing 250 mg L−1 Pb. Single bacterial strains immobilize Pb up to 72%, while in the presence of nematodes, bacterial immobilization potential decreases. An increased Pb immobilization up to 185 mg L−1 is found when bacterial and fungal consortia are inoculated with nematodes. The maximum 80% Pb immobilization is found in bacterial–bacterial combinations with nematodes. This study, reveals that inoculation of bacterial–bacterial consortia in the presence of nematodes enhanced bacterial growth via feeding resulting in higher Pb immobilization. These resistant microbial strains can thus be used to treat contaminated sites for better ecosystem functioning.

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Accelerated temporal turnover of the soil nematode community under alpine grassland degradation

et al. 2022

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Soil nematodes play pivotal roles in the soil food web. However, the trophic regulation on nematodes in the soil micro-food web and its temporal dynamics are less explored. Here, we investigated the seasonal dynamics of soil nematode community in an alpine grassland on the Qinghai-Tibet Plateau at non-degraded (ND), moderately degraded (MD) and severely degraded (SD) stages, either under open-top chamber warming (W) or not (NW). Nematode richness was higher in SD than in MD and ND that did not differ, accompanied by a clear shift from the bottom-up regulation via belowground productivity in ND to the top-down regulation via omnivore and predator nematodes in SD. The intensified top-down regulation in SD was explained by increased proportions of predator and omnivore nematodes, likely induced by the expanded soil pore size in SD. As a result, accelerated temporal turnover of nematode community was observed under grassland degradation, which were 0.006 (slope, p = 0.78), 0.045 (p = 0.07), and 0.077 (p = 0.001) in ND, MD, and SD, respectively. Moreover, grassland degradation strengthened the association between nematode density and ecosystem respiration, whose slope was higher in SD (0.0025, p < 0.05) than in MD (0.0008, p < 0.01) or ND (insignificant). In contrast, warming did not affect the temporal turnover of the nematode community, richness, nor its role in ecosystem respiration. Overall, we revealed the accelerated temporal turnover of nematode community and its strengthened role in determining ecosystem respiration under alpine grassland degradation, suggesting that trophic cascade changes under ecosystem degradation may alter the ecosystem functioning.Kui Wang and Kai Xue contributed equally to this study.

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Dynamic changes of bacterial community under the influence of bacterial-feeding nematodes grazing in prometryne contaminated soil

Cited by 23 publications

References 46 publications

The Variations in the Soil Enzyme Activity, Protein Expression, Microbial Biomass, and Community Structure of Soil Contaminated by Heavy Metals

The Variations in the Soil Enzyme Activity, Protein Expression, Microbial Biomass, and Community Structure of Soil Contaminated by Heavy Metals

The Fate of Lead (Pb) in Multitrophic Interactions Among Bacteria, Fungi, and Bacterivorous Soil Nematodes

Accelerated temporal turnover of the soil nematode community under alpine grassland degradation

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