Dynamic changes of histone H3 trimethylated at positions K4 and K27 in human oocytes and preimplantation embryos

Zhang, Aijun; Xu, Bufang; Sun, Yijuan; Lu, Xiaowei; Gu, Ruihuan; Wu, Ling; Feng, Yun; Xu, Chen

doi:10.1016/j.fertnstert.2012.06.034

Cited by 30 publications

(25 citation statements)

References 36 publications

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“…Results presented as percentage of total DNA ( *,a,b,c, †, ‡ P<0.05). the 8-cell stage and another increase to the blastocyst stage (Zhang et al 2012). These authors suggest the changes observed for both H3K4me3 and H3K27me3 from the 4-cell to 8-cell stage may be associated with embryonic genome activation, (Zhang et al 2012) which occurs during this time in human embryos (Dobson et al 2004).…”

Section: Discussionmentioning

confidence: 90%

“…In addition, the enzymes that methylate H3K27 follow a similar pattern (Ross et al 2008). In human embryos, H3K27me3 is relatively static to the 8-cell stage, where it declines, increasing again at the blastocyst stage (Zhang et al 2012). In mice blastocysts, H3K27me3 is asymmetrically distributed between the ICM and trophoblast cells, as well as being enriched on the inactive X chromosome in the trophectoderm (Erhardt et al 2003, Dahl et al 2010.…”

Section: Immunofluorescene Of H3r26me2 During In Vitro Developmentmentioning

confidence: 93%

“…In mice blastocysts, H3K27me3 is asymmetrically distributed between the ICM and trophoblast cells, as well as being enriched on the inactive X chromosome in the trophectoderm (Erhardt et al 2003, Dahl et al 2010. Human embryos also have dynamic changes in the permissive histone mark, H3K4me3 during development to the blastocyst stage (Zhang et al 2012). These authors observed an increase in H3K4me3 from the pronuclear stage to the 4-cell stage, followed by a decline at cell stage had the highest levels of H3R26me2, followed by 4-cell and morula stage embryos (Fig.2).…”

Section: Immunofluorescene Of H3r26me2 During In Vitro Developmentmentioning

confidence: 99%

“…Histone modification is one such epigenetic mechanism that is critical to embryo development of several species such as mice (Corry et al 2009), cattle (Santos et al 2003, Enright et al 2005, Wee et al 2006, Maalouf et al 2008, Ross et al 2008, pig (Park et al 2009, Gao et al 2010, sheep (Hou et al 2008) and human (Zhang et al 2012). In mice, Sarmento et al (2004), describes two types of histone patterns during preimplantation development, a consistent, stable mark during development and a dynamic, reversible mark.…”

Section: Introductionmentioning

confidence: 99%

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Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

et al. 2015

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In vitro production (IVP) of bovine embryos is not only of great economic importance to the cattle industry, but is also an important model for studying embryo development. The aim of this study was to evaluate the histone modification, H3R26me2 during pre-implantation development of IVP bovine embryos cultured with or without serum supplementation and how these in vitro treatments compared to in vivo embryos at the morula stage. After in vitro maturation and fertilization, bovine embryos were cultured with either 0 or 2.5% fetal bovine serum (FBS). Development was evaluated and embryos were collected and fixed at different stages during development (2-, 4-, 8-, 16-cell, morula and blastocyst). Fixed embryos were then used for immunofluorescence utilizing an antibody for H3R26me2. Images of stained embryos were analyzed as a percentage of total DNA. Embryos cultured with 2.5% FBS developed to blastocysts at a greater rate than 0%FBS groups (34.85±5.43% vs. 23.38±2.93%; P<0.05). Levels of H3R26me2 changed for both groups over development. In the 0%FBS group, the greatest amount of H3R26me2 staining was at the 4-cell (P<0.05), 16-cell (P<0.05) and morula (P<0.05) stages. In the 2.5%FBS group, only 4-cell stage embryos were significantly higher than all other stages (P<0.01). Morula stage in vivo embryos had similar levels as the 0%FBS group, and both were significantly higher than the 2.5%FBS group. These results suggest that the histone modification H3R26me2 is regulated during development of pre-implantation bovine embryos, and that culture conditions greatly alter this regulation.

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Section: Discussionmentioning

confidence: 90%

Section: Immunofluorescene Of H3r26me2 During In Vitro Developmentmentioning

confidence: 93%

Section: Immunofluorescene Of H3r26me2 During In Vitro Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

et al. 2015

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“…In addition, epigenetic mechanisms appear to be highly vulnerable to culture conditions [20,24,25]. Histone modification is an epigenetic mechanism critical to embryo development of several species [26][27][28][29], including cattle [30][31][32][33][34]. Two types of histone patterns during preimplantation development have been described: a consistent stable mark and a dynamic reversible mark [35].…”

Section: Introductionmentioning

confidence: 99%

Oxygen tension affects histone remodeling of in vitro–produced embryos in a bovine model

et al. 2015

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Dynamic changes of histone acetylation and methylation in bovine oocytes, zygotes, and preimplantation embryos

Wang

et al. 2020

J Exp Zool Pt B

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Histone modifications play important roles in regulating chromatin dynamic changes. In this study, acetylated histone H3 lysine 9 and 18 (H3K9ac and H3K18ac), acetylated histone H4 lysine 5 and 8 (H4K5ac and H4K8ac), tri‐methylation histone H3 lysine 4 (H3K4me3), di‐methylation histone H3 lysine 9 (H3K9me2) are investigated in bovine oocytes, zygote, and preimplantation. During meiosis, H3K9ac and H3K18ac are erased after germinal vesicle breakdown, H4K8ac is erased after metaphase I (MI). Although H4K5ac is erased at MI, it is redetectable after this stage. However, histone methylations have no significant change during meiosis. During fertilization, intensive H4K5ac and H4K8ac are resumed on male and female chromatins at postfertilization 4 and 8 hr, respectively. H3K9ac and H3K18ac are resumed on both male and female chromatins at postfertilization 8 and 12 hr, respectively. H3K4me3 and H3K9me2 gradually increased on male chromatin after postfertilization 8 hr, while these two signals on female chromatin are detectable from postfertilization 2–18 hr. During embryo cleavage, H3K9ac, H3K18ac, and H3K4me3 are reduced at 8‐cell stage, and then start to increase. H4K5ac, H4K8ac, and H3K9me2 increase after the 4‐cell stage. At interphase, H4K5ac and H4K8ac are more intensive in nuclear periphery from 2‐ to 8‐cell stages. During mitosis, the signal of H4K8ac is intensive at chromosome periphery. In summary, during both oocyte meiosis and fertilization, the dynamic changes of both histone acetylations and methylations happen in a process of lysine residue‐specific and species‐specific. During preimplantation development, the dynamic patterns of both H3K9ac and H3K18ac are similar to that of H3K4me3, while the dynamic pattern of H4K5ac is similar to that of H4K8ac. These results will be helpful for understanding the effect of histone posttranslational modifications on bovine reproduction and development.

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Dynamic changes of histone H3 trimethylated at positions K4 and K27 in human oocytes and preimplantation embryos

Cited by 30 publications

References 36 publications

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

Oxygen tension affects histone remodeling of in vitro–produced embryos in a bovine model

Dynamic changes of histone acetylation and methylation in bovine oocytes, zygotes, and preimplantation embryos

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