Dynamic Component Chemiluminescent Sensor for Assessing Circulating Polymorphonuclear Leukocyte Activity of Peritoneal Dialysis Patients

Abstract

Recurrent bacterial peritonitis is a major complication in peritoneal dialysis (PD) patients, which is associated with polymorphonuclear leukocyte (PMN) functional changes and can be assessed by a chemiluminescent (CL) reaction. We applied a new approach of a dynamic component chemiluminescence sensor for the assessment of functional states of PMNs in a luminol-amplified whole-blood system. This method is based on the evaluation of CL kinetic patterns of stimulated PMNs, while the parallel measurements of intracellular and extracellular production of reactive oxygen species (ROS) from the same sample can be conducted. Blood was drawn from diabetic and nondiabetic patients during follow-up, and during peritonitis. Healthy medical personnel served as the control group. Chemiluminescence curves were recorded and presented as a sum of three biological components. CL kinetic parameters were calculated, and functional states of PMNs were assessed. Data mining algorithms were used to build decision tree models that can distinguish between different clinical groups. The induced classification models were used afterward for differentiating and classifying new blind cases and demonstrated good correlation with medical diagnosis (84.6% predictive accuracy). In conclusion, this novel method shows a high predictive diagnostic value and may assist in detection of PD-associated clinical states.

Chemiluminescence of non‐differentiated THP‐1 promonocytes: developing an assay for screening anti‐inflammatory milk proteins and peptides

“…In human promyelocytic leukemia cell lines HL‐60 and PLB 985 priming with GM‐CSF resulted in an acceleration of OZ‐induced CL reaction, seen as a 25–40% higher CL slope (28). Shorter OZ‐induced CL peaktimes were observed in whole blood neutrophils of diabetic peritoneal dialysis patients with and without acute peritonitis (40). We have observed quite similar accelerated neutrophil CL reaction kinetics in patients suffering from various respiratory infections (unpublished).…”

Differentiation between Viral and Bacterial Acute Infections Using Chemiluminescent Signatures of Circulating Phagocytes

“…Samples, at a total volume of 200 μL, contained whole fresh blood diluted in KRP, luminol, and zymosan in KRP as was described before. 14 After addition of opsonized zymosan, the sample contents were mixed and CL was measured. Three CL systems were investigated: standard, priming, and aging as was described before 14 and mentioned in Supporting Information.…”

“…14 After addition of opsonized zymosan, the sample contents were mixed and CL was measured. Three CL systems were investigated: standard, priming, and aging as was described before 14 and mentioned in Supporting Information.…”

“…30À32 For each patient, 82 kinetic parameters derived from three systems (standard, priming, and aging systems) were calculated, while the kinetic parameters were published before. 14 The parameters calculated for each specific curve are indicated in Table S-2 of the Supporting Information.…”

“…84.6% predictive accuracy. 14 The phagocytic function resulting from viral exposure was studied previously, including the resulting CL response produced as a result of viral infection. 19À24 A number of reports have also shown altered metabolic activity in PMNs in response to bacterial infection.…”