Dynamic Contrast-Enhanced MRI Measurements of Passive Permeability through Blood Retinal Barrier in Diabetic Rats

Berkowitz, Bruce A.; Roberts, Robin; Luan, Hongmei; Peysakhov, J.; Mao, Xianzhi; Thomas, Kenneth A.

doi:10.1167/iovs.03-1381

Cited by 57 publications

(71 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All patients were well enough to attend as outpatients. The BBB imaging technique was similar to that used previously in the brain, 15 retina, 16 and breast. 17 We tested 2 temporal modifications of the technique in 2 consecutive cohorts, both including patients with lacunar and cortical ischemic strokes.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Changes in Background Blood–Brain Barrier Integrity Between Lacunar and Cortical Ischemic Stroke Subtypes

Wardlaw

Farrall

Armitage

et al. 2008

Stroke

View full text Add to dashboard Cite

Background and Purpose-Lacunar stroke is associated with endothelial dysfunction and histologically with intrinsic cerebral microvascular disease of unknown cause. Endothelial dysfunction could impair blood-brain barrier integrity.We assessed background blood-brain barrier leakage in patients with lacunar ischemic stroke compared with cortical stroke controls. Methods-We recruited patients with lacunar or mild cortical ischemic stroke and assessed generalized cerebral blood-brain barrier leak with MRI and intravenous gadolinium at least 1 month after stroke. We used detailed image processing to compare signal change before and for 30 minutes postcontrast throughout gray matter, white matter, and cerebrospinal fluid with summary analyses and general linear modeling. Results-Among 48 patients (29 lacunar, 19 cortical), postcontrast enhancement was significantly higher in cerebrospinal fluid (Pϭ0.04, Mann-Whitney U), and nonsignificantly higher in white matter, in lacunar than in cortical strokes, with no difference in gray matter. General linear modeling confirmed significantly greater postcontrast enhancement in cerebrospinal fluid in lacunar patients than in cortical controls (tϭ3.37, PϽ0.0008). Conclusion-These preliminary data suggest that the blood-brain barrier may be dysfunctional throughout subcortical white matter (white matter drains via interstitial spaces to cerebrospinal fluid) in patients with lacunar stroke. Further studies are required to confirm these findings and determine whether abnormal blood-brain barrier might predate development of lacunar disease. Blood-brain barrier dysfunction may be an important mechanism for brain damage in cerebral microvascular disease.

show abstract

Section: Methodsmentioning

confidence: 99%

“…15 In the retina (developmentally related to the brain), Gd-DTPA provided a sensitive, noninvasive, and linear assay of passive blood-retinal barrier leak. 16 …”

Section: Methodsmentioning

confidence: 99%

Changes in Background Blood–Brain Barrier Integrity Between Lacunar and Cortical Ischemic Stroke Subtypes

Wardlaw

Farrall

Armitage

et al. 2008

Stroke

View full text Add to dashboard Cite

show abstract

“…It has been established that DCE-MRI measurements of BRB PS are capable of detecting subtle changes in vascular permeability (2). Further, quantifiable changes in BRB permeability have proved useful for the assessment of various therapeutic interventions, including corticosteroid (triamcinolone acetonide) treatment and anti-vascular endothelial growth factor therapy (2,32).…”

Section: Brb Psmentioning

confidence: 99%

“…To avoid these potentially confounding factors, including the near-death artifact, DCE-MRI was applied to rats that were diabetic for 2, 4, 6, or 8 months (2). No evidence of passive BRB leakage in rats was found until after 6 months of diabetes ( Fig.…”

Section: Brb Psmentioning

confidence: 99%

“…electroretinogram), or do not have a simple relationship to disease [e.g. blood-retinal barrier (BRB) disruption and retinal edema (1)(2)(3)]. Furthermore, translating these non-invasive methods into experimental retinopathy rat and mouse models can be difficult because of the generally smaller size of the rodent eye relative to the human eye.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prognostic MRI biomarkers of treatment efficacy for retinopathy

Berkowitz

Roberts

2008

NMR in Biomedicine

Self Cite

View full text Add to dashboard Cite

There is a pressing need for retina-specific imaging biomarkers that robustly measure early (subclinical) changes in physiology, are linked to the histopathology responsible for vision loss, and, importantly, predict treatment efficacy. This review focuses on the following four MRI markers that we have developed and applied in preclinical and clinical settings: preretinal vitreous oxygen level (a steady-state biomarker of inner retinal oxygen tension); leakage of contrast agent into the vitreous (a steady-state biomarker of blood-retinal barrier permeability surface area product); change in preretinal vitreous oxygen tension during a hyperoxic provocation (a functional biomarker of vascular autoregulation); and retinal uptake of systemically administered manganese during a visual task (a functional biomarker of intraretinal ion regulation). We conclude that functional biomarkers are most promising for prognostic evaluation of treatment efficacy earlier in the course of retinopathy than is currently possible.

show abstract