Dynamic de novo adipose tissue development during metamorphosis in Drosophila melanogaster

Abstract

Adipose tissue is a central organ for controlling systemic metabolism both in invertebrates and vertebrates. Here, we have investigated the cellular mechanisms of the adult-type fat body (AFB) development in Drosophila. We have established genetic tools that allow visualization and genetic manipulations of cells in the AFB lineage from early in metamorphosis. We identified precursor cells that give rise to the AFB and delineated dynamic cellular mechanisms underlying AFB formation. These precursor cells displayed polarized cell shapes and oriented motility, with emigration from the thorax and subsequent dispersal to the abdomen and head. After the migration period, these cells adhered to each other, assembling into the AFB with a sheet-like architecture. Continuous cell proliferation occurred during and after the large-scale migration to achieve appropriate fat tissue mass. Homotypic cell fusion after the sheet formation contributed to the establishment of multinucleated cells in the AFB. We also examined candidate gene functions, and our results argue that Rac1, ecdysone signaling, and the transcription factor Serpent support adult fat body organogenesis.Brief Summary StatementDrosophila adult fat body precursor cells form adult adipose tissue during metamorphosis by directional migration, continuous cell proliferation, and homotypic cell fusion.

A single-cell 3D spatiotemporal multi-omics atlas fromDrosophilaembryogenesis to metamorphosis

“…Target genes in the regulons of srp and crp largely overlapped in fat body and plasmatocytes, and this overlap increased along developmental trajectories of both tissues (Figure 4F). The regulons of srp we identified were consistent with its role in inducing fat cell formation starting from early fat body development 49,65 and crp is known to affect cell growth and tissue size control 56 .…”

Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adultDrosophila melanogasteradipose tissue

“…Derived from mesoderm, the larval fat body exists throughout the anterior-posterior axis as a sheet of tightly adhered polygonal adipocytes that dissociate into individual spherical cells at the larval-to-pupal transition (Zheng et al, 2016). During the pupal-to-adult transistion, adipocyte precursors migrate from the thorax to the abdomen, proliferate, and undergo fusion to generate the adult fat body (Tsuyama et al, 2023). While the vast majority of fat body mass is located in the abdomen, adipose tissue is also found in the head (pericerebral fat) and thorax (Roman et al, 2001).…”