Dynamic evaluation of cell-secreted interferon gamma in response to drug stimulation via a sensitive electro-chemiluminescence immunosensor based on a glassy carbon electrode modified with graphene oxide, polyaniline nanofibers, magnetic beads, and gold nanoparticles

Zhu, Mingyao; Tang, Yanjuan; Wen, Qingqing; Li, Jiong; Yang, Peihui

doi:10.1007/s00604-016-1804-9

Cited by 35 publications

(18 citation statements)

References 46 publications

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“…The microspheres ampli ed the optical signal of speci c antigen-antibody binding and the sensitivity is improved compared with other labelled technologies [39,40]. This method avoids shortcomings of time consumption and the use of accurate and expensive instruments, with advantages of rapid on-site detection, low cost, and no special operation requirements [41,42].…”

Section: Discussionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

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Background: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic test strip (ICTS) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum.Result: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The detection results were achieved by using the ratio of the fluorescence signals of the test and control lines (HT/HC) with a threshold of 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICTS and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, and no cross reaction with the sera of other related diseases was observed.Conclusion: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost.

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Section: Discussionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

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“…Although ELISA and PCR-based quantification seem to be the methods of choice in the current development of stem cell-based products due to their outstanding detection limits, the challenge of guaranteeing high quality measurements is increasingly being met by progress in biosensor design. Various functionalized forms of graphene and nanocomposites [ 41 ] can be used to develop biosensors for a broad range of probes suitable for recognizing specific biomarkers relevant to stem cell-based therapies, including hallmarks of vascularization (e.g., VEGF [ 42 ]), proliferation and differentiation (e.g., miR-21 [ 43 ] and Bcl-2 [ 44 ]), immunity (e.g., TNF- α [ 45 ] and IFN- γ [ 46 ]) or apoptosis (e.g., caspase-3 activity [ 47 ]), and pluripotency factors (e.g., NANOG [ 48 ]). Graphene-based materials include pristine graphene, functionalized forms such as graphene oxide (GO), reduced graphene oxide (rGO), and graphene quantum dots (GQD) can each introduce particular charge interaction qualities utilized by sensing platforms [ 49 ].…”

Section: Graphene-based Platforms To Accelerate Potency Assay Devementioning

confidence: 99%

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

Amărandi

Becheru

Vlăsceanu

et al. 2018

BioMed Research International

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Regenerative medicine is challenged by the need to conform to rigorous guidelines for establishing safe and effective development and translation of stem cell-based therapies. Counteracting widespread concerns regarding unproven cell therapies, stringent cell-based assays seek not only to avoid harm but also to enhance quality and efficacy. Potency indicates that the cells are functionally fit for purpose before they are administered to the patient. It is a paramount quantitative critical quality attribute serving as a decisive release criterion. Given a broad range of stem cell types and therapeutic contexts the potency assay often comprises one of the most demanding hurdles for release of a cell therapy medicinal product. With need for improved biomarker assessment and expedited measurement, recent advances in graphene-based biosensors suggest that they are poised to be valuable platforms for accelerating potency assay development. Among several potential advantages, they offer versatility for sensitive measurement of a broad range of potential biomarker types, cell biocompatibility for direct measurement, and small sample sufficiency, plus ease of use and point-of-care applicability.

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“…Bio-functionalized MNPs, that is, immune magnetic beads (IMB), are frequently utilized in cell sorting, bio-separation, targeted drug delivery, and immunoassays. This method can also be used for the enrichment and separation of bacteria, which avoids the shortcomings of time-consuming, pre-enrichment and has the advantages of simple operation, speed and high efficiency [18,19]. However, there are very few relevant reports about the application of nano-detection technology in the field of disease diagnosis, especially in clinical sample detection.…”

Section: Introductionmentioning

confidence: 98%

A sandwich immunoassay for brucellosis diagnosis based on immune magnetic beads and quantum dots

Yin

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

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Dynamic evaluation of cell-secreted interferon gamma in response to drug stimulation via a sensitive electro-chemiluminescence immunosensor based on a glassy carbon electrode modified with graphene oxide, polyaniline nanofibers, magnetic beads, and gold nanoparticles

Cited by 35 publications

References 46 publications

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Advantages of Graphene Biosensors for Human Stem Cell Therapy Potency Assays

A sandwich immunoassay for brucellosis diagnosis based on immune magnetic beads and quantum dots

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