In the recent past, several researchers have successfully modeled the complex fatigue behavior of planar twin-roll cast AZ31B alloy sheets. Complex components are usually hot-bent, whereby the microstructure in the hot-bent areas changes significantly. However, studies on the fatigue behavior of hot-bent magnesium alloys are currently lacking. Therefore, a novel, uniaxial hot-bent specimen was developed and optimized with finite element method simulations. Microstructural analyses with the electron backscatter diffraction method reveal that the hot-bending process changes the texture and increases the Schmid factor for basal slip in rolling and transverse direction of the sheet. In the subsequent quasi-static tension and compression tests, anisotropic and asymmetric yield stresses, lower Young’s moduli compared with the as-received material and macroscopic bands of twinned grains are obtained. Finally, the study proves that the recently proposed concept of highly strained volume can accurately estimate the lifetime, even by combining the as-received and hot-bent material in one fatigue model.