Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens

Abstract

Wan Y., Ma C., Wei P., Fang Q., Guo X., Zhou B., Jiang R. (2017): Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens. Czech J. Anim. Sci., 62, 82-87.The effects of heat stress on HSP90B1 messenger RNA (mRNA) expression in the hypothalamus of chicken were investigated and HSP90B1 variations were detected. Females of two Chinese chicken breeds (Huainan and Wenchang) were used for the experiments. At 64 days of age, the ambient temperature (24 ± 1°C) was increased to 35 ± 1°C for 24 h (heat stress), then decreased to 24 ± 1°C for 24 h (recovery). Hypothalamus samples were collected at 0, 12, and 24 h during heat stress, as well as 12 and 24 h during recovery. The HSP90B1 mRNA expression increased significantly during heat stress and significantly decreased during recovery being higher in Huainan chickens. Fifteen primer pairs were designed to amplify the exons of HSP90B1 by a polymerase chain reaction, and single nucleotide polymorphisms (SNPs) were detected by Sanger sequencing. In Huainan chickens, we identified a SNP (NC_006088.3:g.6798G>A) in exon 14 of HSP90B1 which did not cause amino acid variation but caused a codon for glutamic acid change from GAG to GAA. The frequencies for genotypes AA, GA, and GG were 0.49, 0.27, and 0.24, respectively. Individuals with the GG genotype survived heat stress at 42°C for a longer time (248.2 ± 39.3 min) than individuals with GA and AA genotypes, which survived for 227.2 ± 44.5 min and 179.3 ± 36.5 min, respectively. The results suggested that the increased heat tolerance was associated with the higher expression of HSP90B1, and genotype GG could be used as a potential marker for heat resistance in chickens.

HSP70 And HSP90β Genes Polymorphism And Its Association With Thermotolerance In Fayoumi And Leghorn Chicken Breeds

“…Our results are also consistent with these finding since our results revealed that Fayoumi chickens has only the wild genotype of HSP90β gene in both susceptible and resistant birds. Wan et al, (2017) identified G 6798A SNP in HSP90B1 gene that produced three genotypes. The chickens with GG genotype have longer survival time than individuals with the other two genotypes, indicating that allele G was favorable for heat resistance and has potentially to be used in improving heat resistance.…”

“…In Lingshan and White Recessive Rock chickens G141A SNP in the HSP90β gene 5' promoter region was existing in chickens with a great heat tolerance (Chen et al, 2013). Wan et al, (2017) identified polymorphisms in the HSP90AB1 gene, they explained that G allele single nucleotide polymorphism at G6798A occurring in exon 14 improved heat tolerance and displayed a longer survival in Huainan chickens during heat stress. These SNPs in HSP70 and HSP90 genes have ability to be used in breeding programs for improving chickens heat tolerance.…”

Involvement of Cholesterol, Progesterone, Cortisol and Lipoproteins in Metabolic Changes During Early Ontogenesis of Broiler Chicks of an Industrial Cross

“…The endocrine regulation serves as the basis of homeostasis, formed during the species evolution, and as the primary link between endo-and exogenous flows of energy, substrates, and signaling pathways. It includes a hierarchical chain of hormones; the blood content of these hormones according to the type of feedback determines, among other things, the amount of blood cholesterol and lipoproteins, which reflect both the processes of synthesis of adrenal hormones and their regulatory effects [2,[8][9][10]. One of these hormones, the progesterone synthesized from low-density lipoprotein cholesterol, is best known for its participation in the endocrine control of the formation, growth and development of the fetus.…”

“…They also participate in the neurotransmitter control over the chronological rhythms of postnatal growth and development due to receptor interaction with aminobutyric acid [12,13] and dopamine, affect the organogenesis of blood vessels, locomotor apparatus [15], skeletal muscles (which has hypertrophic development in broiler chickens) [5,9], and myelination of nerve fibers [13]. Progesterone, 17-hydroxyprogesterone and its metabolite cortisol are among the primary activators and effectors of the hypothalamic-pituitary-adrenal axis [10,16]. However, the information on the role of progesterone and its derivatives in lipid and protein metabolisms (despite the direct link between the biosynthesis of these metabolites, particularly the low and high density lipoproteins and hormones) is extremely limited.…”

“…Progesterone affects metabolism and increases its intensity in chicks. Cortisol directly participates in complex adaptive responses at molecular, membrane, cellular, tissue, and organ levels [10,16]. Cortisol (see Fig.…”