Dynamic force microscopy imaging of native membranes

Kienberger, Ferry; Stroh, Cordula M.; Kada, Gerald; Moser, Rosita; Baumgärtner, Werner; Pastushenko, V. F.; Rankl, Christian; Schmidt, Ute; Müller, H.‐Arno J.; Orlova, Elena V.; LeGrimellec, Christian; Drenckhahn, Detlev; Blaas, Dieter; Hinterdorfer, Peter

doi:10.1016/s0304-3991(03)00047-0

Cited by 63 publications

(31 citation statements)

References 32 publications

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“…21 Topography images were acquired using magnetic ACmode (MACmode) AFM, where an alternating magnetic field drives the oscillation of a magnetically coated cantilever 22,23 (Figure 1(a)-(c)). sendai-PM were 5(G1) nm in height and exhibited a hexagonal arrangement of trimeric bacteriorhodopsin (BR) molecules, similar to the appearance of wild-type PM.…”

Section: Resultsmentioning

confidence: 99%

Single Molecule Studies of Antibody–Antigen Interaction Strength Versus Intra-molecular Antigen Stability

Kienberger

Kada

Mueller

et al. 2005

Journal of Molecular Biology

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106

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Section: Resultsmentioning

confidence: 99%

Single Molecule Studies of Antibody–Antigen Interaction Strength Versus Intra-molecular Antigen Stability

Kienberger

Kada

Mueller

et al. 2005

Journal of Molecular Biology

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106

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“…In order to reduce the overall displacement during imaging, immobilization procedures for tight and non-invasive anchoring of cells to substrates have been developed (Schilcher et al, 1997). In addition, dynamic force microscopy (DFM) methods like tappingmode (Putman et al, 1994) and MACmode AFM (Han et al, 1996) have greatly reduced the problem of lateral forces responsible for tip-induced deformation of soft biological samples (Kienberger et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the advantage of DFM is that lateral friction forces during imaging are significantly reduced, thereby minimizing sample damage by the scanning cantilever (Kienberger et al, 2004). Imaging of biological specimens has therefore significantly benefited from the development of DFM, since soft samples are less deformed and specimens weakly adhered to surfaces are not so easily displaced by the scanning cantilever (Kienberger et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…For the binding of RBCs to the WGA-modified surface, RBCs were diluted 1:60 using PBS and adsorbed for 20 min on the mica sheets, followed by washing with PBS and fixation of immobilized RBCs with 1% glutaraldehyde for 1 min. After removal of the fixation agent by washing with the same buffer, AFM images (acoustic excitation with a multimode Nanoscope IIIa, Veeco) were acquired in a fluid cell at room spring constant) cantilever, the oscillation amplitude and the amplitude reduction during imaging were minimized to reduce the indentation force of the scanning tip (Kienberger et al, 2003). A careful adjustment of the imaging parameters during scanning is known to be essential for the successful imaging of thick and soft materials like whole cells (Vie et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM

Kamruzzahan

Kienberger

Stroh

et al. 2004

Biological Chemistry

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The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly tethered to the support. This results in a tight but non-invasive attachment of the cells. Using tappingmode AFM, which is known as gentle imaging mode and therefore most appropriate for soft biological samples like erythrocytes, it was possible to resolve membrane skeleton structures without major distortions or deformations of the cell surface. Significant differences in the morphology of RBCs from healthy humans and patients with systemic lupus erythematosus (SLE) were observed on topographical images. The surface of RBCs from SLE patients showed characteristic circular-shaped holes with approx. 200 nm in diameter under physiological conditions, a possible morphological correlate to previously published changes in the SLE erythrocyte membrane.

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“…However, contact-mode imaging turned out to be less suitable for weakly attached samples, because biomolecules are often pushed away by the AFM stylus during scanning (12). To overcome this disadvantage, dynamic force microscopy (DFM) methods such as tapping-mode AFM, and more recently, magnetic AC mode AFM (MACmode AFM) (5) have been used for imaging soft and weakly attached biological samples (14,17). For DFM, the cantilever oscillates and touches the sample only intermittently at the end of its downward movement, which reduces the contact time and minimizes friction forces.…”

mentioning

confidence: 99%

Monitoring RNA Release from Human Rhinovirus by Dynamic Force Microscopy

Kienberger¹,

Zhu

Moser

et al. 2004

J Virol

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Human rhinoviruses were imaged under physiological conditions by dynamic force microscopy. Topographical images revealed various polygonal areas on the surfaces of the 30-nm viral particles. RNA release was initiated by exposure to a low-pH buffer. The lengths of the RNAs that were released but still connected to the virus capsid varied between 40 and 330 nm, whereas RNA molecules that were completely released from the virus were observed with lengths up to 1 m. Fork-like structure elements with 30-nm extensions were sometimes resolved at one end of the RNA molecules. They possibly correspond to the characteristic multistem-loop conformation, the internal ribosomal entry site, located at the 5 region of the genome. This study demonstrates that dynamic force microscopy can be used to study viral RNA release in situ under physiological conditions.

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Dynamic force microscopy imaging of native membranes

Cited by 63 publications

References 32 publications

Single Molecule Studies of Antibody–Antigen Interaction Strength Versus Intra-molecular Antigen Stability

Single Molecule Studies of Antibody–Antigen Interaction Strength Versus Intra-molecular Antigen Stability

Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM

Monitoring RNA Release from Human Rhinovirus by Dynamic Force Microscopy

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