Dynamic interactions between peroxidase-mimic silver NanoZymes and chlorpyrifos-specific aptamers enable highly-specific pesticide sensing in river water

Weerathunge, Pabudi; Behera, Bijay Kumar; Zihara, Sabna; Singh, Mandeep; Prasad, Sanjana Naveen; Hashmi, Sabeen; Mariathomas, Pyria Rose Divina; Bansal, Vipul; Ramanathan, Rajesh

doi:10.1016/j.aca.2019.07.066

Cited by 95 publications

(44 citation statements)

References 67 publications

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“…The nanozyme signaling methodology of Weerathunge et al [ 120 ] mentioned in section 9.2.4 . for NoV was extended to chlorpyrifos (Chl), an organophosphorus pesticide, by some of these same investigators in another report [ 122 ]. The underlying working principle of the aptasensor is based on the dynamic non-covalent interaction of a Chl-specific aptamer with the intrinsic peroxidase-mimic nanozyme activity of tyrosine-capped silver (Ag) NPs.…”

Section: Food and Water Analysismentioning

confidence: 99%

Recent advances in aptamer applications for analytical biochemistry

Zon

2022

Analytical Biochemistry

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Aptamers are typically defined as relatively short (20–60 nucleotides) single-stranded DNA or RNA molecules that bind with high affinity and specificity to various types of targets. Aptamers are frequently referred to as “synthetic antibodies” but are easier to obtain, less expensive to produce, and in several ways more versatile than antibodies. The beginnings of aptamers date back to 1990, and since then there has been a continual increase in aptamer publications. The intent of the present account was to focus on recent original research publications, i.e., those appearing in 2019 through April 2020, when this account was written. A Google Scholar search of this recent literature was performed for relevance-ranking of articles. New methods for selection of aptamers were not included. Nine categories of applications were organized and representative examples of each are given. Finally, an outlook is offered focusing on “faster, better, cheaper” application performance factors as key drivers for future innovations in aptamer applications.

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Section: Food and Water Analysismentioning

confidence: 99%

Recent advances in aptamer applications for analytical biochemistry

Zon

2022

Analytical Biochemistry

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“…Environmental water samples were spiked with isocarbophos, and demonstrated recoveries ranged from 84 to 97%. Finally, enzyme-based colorimetric detection of chlorpyrifos was achieved through the use of a NanoZyme (Weerathunge et al, 2019). Briefly, tyrosine-capped silver nanoparticles have intrinsic peroxidasemimicking activity, which was controlled by the interaction of a chlorpyrifos specific aptamer and the surface of the nanoparticle.…”

Section: Pesticides and Insecticidesmentioning

confidence: 99%

“…Originally described by the World Health Organization as the ideal characteristics for point-of-care testing in low-resource settings, the ASSURED criteria stand as an excellent benchmark for the development of biosensors for environmental monitoring as well. These criteria state that in order to be practical for use, a biosensor should be affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable (Weerathunge et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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Due to their relative synthetic and chemical simplicity compared to antibodies, aptamers afford enhanced stability and functionality for the detection of environmental contaminants and for use in environmental monitoring. Furthermore, nucleic acid aptamers can be selected for toxic targets which may prove difficult for antibody development. Of particular relevance, aptamers have been selected and used to develop biosensors for environmental contaminants such as heavy metals, small-molecule agricultural toxins, and water-borne bacterial pathogens. This review will focus on recent aptamer-based developments for the detection of diverse environmental contaminants. Within this domain, aptamers have been combined with other technologies to develop biosensors with various signal outputs. The goal of much of this work is to develop cost-effective, user-friendly detection methods that can complement or replace traditional environmental monitoring strategies. This review will highlight recent examples in this area. Additionally, with innovative developments such as wearable devices, sentinel materials, and lab-on-a-chip designs, there exists significant potential for the development of multifunctional aptamer-based biosensors for environmental monitoring. Examples of these technologies will also be highlighted. Finally, a critical perspective on the field, and thoughts on future research directions will be offered.

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“…A higher number of biosensors utilizing MIP based receptors have been reported for the detection of pesticides, partly due to the convenience of synthesizing MIPs. Recently, Weerathunge et al demonstrated the use of a nanozyme based sensor and aptamer for the detection of chlorpyrifos down to 11.3 ppm (32 µM) [59]. The nanozyme, based on Ag nanoparticles were found to catalyze the 3,30,5,50-tetramethylbenzidine (TMB) substrate, leading to a colour change.…”

Section: Pesticidesmentioning

confidence: 99%

The Use of Aptamers and Molecularly Imprinted Polymers in Biosensors for Environmental Monitoring: A Tale of Two Receptors

et al. 2020

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Effective molecular recognition remains a major challenge in the development of robust receptors for biosensing applications. Over the last three decades, aptamers and molecularly imprinted polymers (MIPs) have emerged as the receptors of choice for use in biosensors as viable alternatives to natural antibodies, due to their superior stability, comparable binding performance, and lower costs. Although both of these technologies have been developed in parallel, they both suffer from their own unique problems. In this review, we will compare and contrast both types of receptor, with a focus on the area of environmental monitoring. Firstly, we will discuss the strategies and challenges involved in their development. We will also discuss the challenges that are involved in interfacing them with the biosensors. We will then compare and contrast their performance with a focus on their use in the detection of environmental contaminants, namely, antibiotics, pesticides, heavy metals, and pathogens detection. Finally, we will discuss the future direction of these two technologies.

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Dynamic interactions between peroxidase-mimic silver NanoZymes and chlorpyrifos-specific aptamers enable highly-specific pesticide sensing in river water

Cited by 95 publications

References 67 publications

Recent advances in aptamer applications for analytical biochemistry

Recent advances in aptamer applications for analytical biochemistry

Aptamer-Based Biosensors for Environmental Monitoring

The Use of Aptamers and Molecularly Imprinted Polymers in Biosensors for Environmental Monitoring: A Tale of Two Receptors

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