Dynamic investigation of zein-based degradable and hemocompatible coatings for drug-eluting stents: a microfluidic approach

Lenzuni, Martina; Bonfadini, Silvio; Criante, Luigino; Zorzi, Filippo; Summa, Maria; Bertorelli, Rosalia; Suarato, Giulia; Athanassiou, Athanassia

doi:10.1039/d3lc00012e

Cited by 7 publications

(2 citation statements)

References 75 publications

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“…According to the “3Rs concept”, every attempt was made to reduce animal suffering and to utilize the fewest number of animals necessary to provide accurate findings. Healthy mouse blood containing heparin (5000 U/mL) was taken and diluted with normal saline (4:5 ratio by volume), following standard procedures . Fresh heparinized blood was stored at room temperature and used within 2 h of collection.…”

Section: Methodsmentioning

confidence: 99%

Schiff Base-Based Hydrogel Embedded with In Situ Generated Silver Nanoparticles Capped by a Hyaluronic Acid–Diethylenetriamine Derivative for Wound Healing Application

Martorana,

Lenzuni,

Contardi

et al. 2024

ACS Appl. Mater. Interfaces

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In this study, hydrogels were produced using a Schiff base reaction between two hyaluronic acid derivatives: one containing aldehyde groups (HA-Ald) and the other holding a diethylenetriamine with terminal amino groups (HA-DETA). The DETA portion promotes the in situ growth, complexation, and stabilization of silver nanoparticles (AgNPs), eliminating the need for external reducing agents. The reaction between HA-DETA and HA-Ald leads to the formation of imine bonds, which results in dynamically pH-responsive cross-linking. While the DETA capping ability helped in embedding the AgNPs, the on/off pH environmental responsivity of the hydrogel allows for a controlled and ondemand release of the drug, mainly when bacterial infections cause pH variation of the wound bed. The injectable hydrogels resulted in being highly compatible in contact with blood red cells, fibroblasts, and keratinocytes and capable of having a proliferative effect on an in vitro wound scratch model. The pH-responsive hydrogels showed proper antibacterial activity againstPseudomonas aeruginosaandStaphylococcus aureus, common bacterial strains presented in wound infections. Finally, in vivo wound model studies demonstrated an overall speeding up in the wound healing rate and advanced wound conditions in the experimental group treated with the hydrogels compared to control samples.

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Section: Methodsmentioning

confidence: 99%

Schiff Base-Based Hydrogel Embedded with In Situ Generated Silver Nanoparticles Capped by a Hyaluronic Acid–Diethylenetriamine Derivative for Wound Healing Application

Martorana,

Lenzuni,

Contardi

et al. 2024

ACS Appl. Mater. Interfaces

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“…Hemocompatibility tests were performed to determine the blood compatibility of the proposed films for wound treatment, according to the ISO 10993‐4 norm and standard practices for evaluating the interaction of a medical device or a material with blood. [ 19 ] C57BL/6 mice ( n = 2) weighing 23−24 g were used to draw blood. All procedures were performed following the Ethical Guidelines of the European Communities Council (Directive 2010/63/EU of 22 September 2010) and accepted by the Italian Ministry of Health.…”

Section: Methodsmentioning

confidence: 99%

Combining Alginate/PVPI‐Based Film with Frequency Rhythmic Electrical Modulation System (FREMS) Technology as an Advanced Strategy for Diabetic Wounds

Contardi,

Summa,

Lenzuni

et al. 2023

Macromolecular Bioscience

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Diabetes is rising as one of the most diffused diseases of our century with the related urgent necessity to face its systemic and local effects on the patients, such as cardiovascular problems, degeneration of limbs, and dysfunction of the wound healing process. The diffusion of leg ulcers has been estimated to be 1.51 for 1,000 population, and these non‐resolved wounds can produce several social, economic, and mental health issues in diabetic patients. At the same time, these people experience neuropathic pain that causes morbidity and a further decrease in their quality of life. Here, we present a new study where an Alginate/PVPI‐based wound dressing is combined with the Frequency Rhythmic Electrical Modulation System (FREMS™) technology, an established medical device for the treatment of neuropathic pain and diabetic ulcers. The produced Alginate/PVPI‐based films were characterized in terms of morphology, chemistry, wettability, bio‐/hemo‐compatibility, and clotting capacity. Next, the Alginate/PVPI‐based films were used together with FREMSTM technology in diabetic mice models, and synergism of their action in the wound closure rate and anti‐inflammatory properties was found. Hence, we want to demonstrate how the combination of electrical neurostimulation devices and advanced wound dressings can be a new approach to improve chronic wound treatment.This article is protected by copyright. All rights reserved

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Composites for Drug-Eluting Devices: Emerging Biomedical Applications

Gupta,

Choudhari,

Kumar

et al. 2024

Applications of Biotribology in Biomedical Systems

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Dynamic investigation of zein-based degradable and hemocompatible coatings for drug-eluting stents: a microfluidic approach

Abstract: Microfluidic-based dynamic assays for drug-eluting stent coatings.

Cited by 7 publications

References 75 publications

Schiff Base-Based Hydrogel Embedded with In Situ Generated Silver Nanoparticles Capped by a Hyaluronic Acid–Diethylenetriamine Derivative for Wound Healing Application

Schiff Base-Based Hydrogel Embedded with In Situ Generated Silver Nanoparticles Capped by a Hyaluronic Acid–Diethylenetriamine Derivative for Wound Healing Application

Combining Alginate/PVPI‐Based Film with Frequency Rhythmic Electrical Modulation System (FREMS) Technology as an Advanced Strategy for Diabetic Wounds

Composites for Drug-Eluting Devices: Emerging Biomedical Applications

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