Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients

Djukić, Tamara; Drvenica, Ivana; Kovačić, Marijana; Minić, Rajna; Vucetic, Dusan; Majerič, Dragana; Sefik-Bukilica, M.; Savić, Olivera; Bugarski, Branko; Ilić, Vesna

doi:10.1016/j.ab.2023.115194

Cited by 1 publication

(8 citation statements)

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“…By analyzing individual samples of serum CICs from RA patients by DLS, we mapped the dominant CIC particle sizes, which generally clustered around two broad-size peaks. This was in accordance with our previous study, where we measured pooled CIC samples [ 15 ]. Additionally, in this study, CIC-containing particles significantly differed in size between the studied groups and were correlated with immunoglobulin concentrations to ascertain any potential associations.…”

Section: Discussionsupporting

confidence: 93%

“…In our previous research, we successfully applied DLS to determine CIC particles’ size in pooled CIC samples of healthy people and RA patients [ 15 ]. Herein, we performed DLS measurements on individual CIC samples for unbiased and highly accurate analysis.…”

Section: Resultsmentioning

confidence: 99%

“…However, collecting synovial fluid is invasive and burdensome and is not commonly used for diagnostic purposes. In our previous study, we successfully demonstrated the feasibility of DLS for the biophysical analysis of pooled ICs isolated from the serum of patients with RA [ 15 ]. This study aimed to measure the size of colloidal particles found in the sera of CICs of individual RA patients using DLS.…”

Section: Discussionmentioning

confidence: 99%

“…The IgA, IgG, or IgM index represents the intensity of IgA, IgG, or IgM dot blot signal normalized to the assay control (pooled healthy human serum; 83 g/L total proteins) diluted to the protein concentration of 2.1 mg/mL, which corresponded to the concentration of 3% PEG-precipitated serum proteins (our laboratory standard). The concentration of IgG, IgA, and IgM in pooled sera was determined by immunonephelometry [ 15 ], and in diluted sera, the concentration was 0.20, 0.04, and 0.02 g/L, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…They showed that IgG- and IgM-containing ICs, isolated by affinitive chromatography from pooled synovial fluid of RA patients, differed in particle size. Inspired by the work of György and others [ 14 ], we have recently demonstrated the feasibility of DLS to identify the RA-specific but also the age-specific distribution of pooled circulating immune complex (CIC) size using sera of RA patients [ 15 ]. Additionally encouraged by the fact that data on the association of RA disease profiles with immunoglobulin classes/subclasses/allotypes are also contradictory [ 16 , 17 ], in this study, we were motivated to determine the size of colloid particles in the CICs of individual RA patients by DLS and to analyze if the presence of ACPAs and RFs in peripheral blood, and the specific immunoglobulin isotypes in isolated CICs, were associated with particular sizes of CIC colloidal particles.…”

Section: Introductionmentioning

confidence: 99%

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Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients

Djukić,

Drvenica,

Kovačić

et al. 2024

IJMS

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The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.

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Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%