Dynamic light scattering optical coherence tomography

Lee, Jong‐Hwan; Wu, Wei‐Cheng; Jiang, James; Zhu, Bin; Boas, David A.

doi:10.1364/oe.20.022262

Cited by 153 publications

(173 citation statements)

References 24 publications

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“…So far those methods have been successfully applied only to well-fixated samples. 151 The reason is the number of correlated scans necessary to perform flow analysis with sufficient dynamics. Bulk motion as well as measurement time might, therefore, be critical for human in vivo applications.…”

Section: Label-free Optical Angiographymentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

432

264

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Abstract. In the last 25 years, optical coherence tomography (OCT) has advanced to be one of the most innovative and most successful translational optical imaging techniques, achieving substantial economic impact as well as clinical acceptance. This is largely owing to the resolution improvements by a factor of 10 to the submicron regime and to the imaging speed increase by more than half a million times to more than 5 million A-scans per second, with the latter one accomplished by the state-of-the-art swept source laser technologies that are reviewed in this article. In addition, parallelization of OCT detection, such as line-field and full-field OCT, has shortened the acquisition time even further by establishing quasi-akinetic scanning. Besides the technical improvements, several functional and contrast-enhancing OCT applications have been investigated, among which the label-free angiography shows great potential for future studies. Finally, various multimodal imaging modalities with OCT incorporated are reviewed, in that these multimodal implementations can synergistically compensate for the fundamental limitations of OCT when it is used alone. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Label-free Optical Angiographymentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

432

264

View full text Add to dashboard Cite

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“…[16][17][18] Dynamic Light Scattering-Optical Coherence Tomography Analysis Technical details of DLS-OCT theory and analysis have been described in our previous publication. 19 In brief, dynamic OCT imaging of a sample produced four-dimensional (space and time) data of the complex-valued reflectivity, R(r, t). The field autocorrelation function was obtained as where M S (r), M F (r), v t (r), v z (r), and D(r) are the parameters of particle dynamics to be estimated for each position, while the others are the parameters given by the measurement system (all physical quantities used in this paper are listed in Supplementary Table S1).…”

Section: Spectral-domain-optical Coherence Tomography System and Scanmentioning

confidence: 99%

Quantitative Imaging of Cerebral Blood Flow Velocity and Intracellular Motility using Dynamic Light Scattering–Optical Coherence Tomography

Lee

Radhakrishnan

et al. 2013

J Cereb Blood Flow Metab

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This paper describes a novel optical method for label-free quantitative imaging of cerebral blood flow (CBF) and intracellular motility (IM) in the rodent cerebral cortex. This method is based on a technique that integrates dynamic light scattering (DLS) and optical coherence tomography (OCT), named DLS-OCT. The technique measures both the axial and transverse velocities of CBF, whereas conventional Doppler OCT measures only the axial one. In addition, the technique produces a three-dimensional map of the diffusion coefficient quantifying nontranslational motions. In the DLS-OCT diffusion map, we observed high-diffusion spots, whose locations highly correspond to neuronal cell bodies and whose diffusion coefficient agreed with that of the motion of intracellular organelles reported in vitro in the literature. Therefore, the present method has enabled, for the first time to our knowledge, label-free imaging of the diffusion-like motion of intracellular organelles in vivo. As an example application, we used the method to monitor CBF and IM during a brief ischemic stroke, where we observed an induced persistent reduction in IM despite the recovery of CBF after stroke. This result supports that the IM measured in this study represent the cellular energy metabolism-related active motion of intracellular organelles rather than free diffusion of intracellular macromolecules.

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“…Our future work will focus on compensating these errors or designing a new high-pass filter with less influence on the Doppler estimation. Also note that in the current study, we did not consider the relation between the degree of correlation and the fidelity of the Doppler signal 16 when imaging the CBF. Such relation can be important for in vivo applications, which might be useful to guide the optimization of scanning patterns to solve the limitations of the current approach.…”

Section: Resultsmentioning

confidence: 94%

Wide velocity range Doppler optical microangiography using optimized step-scanning protocol with phase variance mask

et al. 2013

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Abstract. We propose a simple and optimized method for acquiring a wide velocity range of blood flow using Doppler optical microangiography. After characterizing the behavior of the scanner in the fast scan axis, a step-scanning protocol is developed by utilizing repeated A-scans at each step. Multiple velocity range images are obtained by the high-pass filtering and Doppler processing of complex signals between A-scans within each step with different time intervals. A phase variance mask is then employed to segment meaningful Doppler flow signals from noisy phase background. The technique is demonstrated by imaging in vivo mouse brain with skull left intact to provide bidirectional images of cerebral blood flow with high quality and wide velocity range. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Dynamic light scattering optical coherence tomography

Cited by 153 publications

References 24 publications

Optical coherence tomography today: speed, contrast, and multimodality

Optical coherence tomography today: speed, contrast, and multimodality

Quantitative Imaging of Cerebral Blood Flow Velocity and Intracellular Motility using Dynamic Light Scattering–Optical Coherence Tomography

Wide velocity range Doppler optical microangiography using optimized step-scanning protocol with phase variance mask

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