Dynamic light scattering studies of collagen

Abstract

Monodisperse rat skin collagen solutions obtained through purification by density gradient separation have been studied by dynamic light scattering. Autocorrelation functions were measured at 31.8° and 90° scattering angles. Calculations of the translational diffusion coefficient DT and the rotational diffusion coefficient DR from the measured correlation functions of scattered light have been carried out in this study. A new approach to simulatanously determining the translational and rotational diffusion coefficients was used to estimate both the length of the macromolecules and the viscosity of the solutions. Our result for L is 2715±100 Å.

Dynamic light scattering study of micelles in a high ionic strength solution

“…One other phenomenon which may also affect the dynamic light scattering spectra is that of flexibility. Some authors believe that rodlike micelles are flexible4,5,28 while others dp not.29 For the extremely flexible case, that of a Gaussian coil, an expression similar to eq 8 for the light scattering first-order correlation function is obtained13 and can be written as gO)(T) = S0(x) exp{~q2Dr) + S2(x)exp[-(q2D + 2/ ,) ] + ... (17) where x = q2Ro2, where Ra is the radius of gyration, r, is the relaxation time of the first normal mode of the Gaussian coil, and the S"s are the dynamic form factors. In Figure 2 the form factors Sq and S2 are plotted along with the total form factor S' = ,-S,• and the remaining terms defined by Sh = S-S0-S2 (18) as a function of x.…”

The Complex of Tests for the Quantitative Evaluation of the Effects of Radiation on Laboratory Animals

“…Laser correlation spectroscopy (LCS) (analogs: spectroscopy of quasi-elastic light scatter, optical mixing spectroscopy, photon-correlation spectroscopy, dynamic light scattering) consists in measurement of spectral characteristics of light scattered in quasi-elastic mode from the spectrum of intensity fluctuations of the recorded light [11][12][13][14][15].…”

Laser correlation spectroscopy (LCS) and its clinical perspectives in ophthalmology

“…Laser correlation spectroscopy (LCS) (analogs: spectroscopy of quasi-elastic light scatter, optical mixing spectroscopy, photon-correlation spectroscopy) consists of measurement of spectral characteristics of light scattered in quasi-elastic mode from the spectrum of fluctuation intensity of the recorded light (4)(5)(6)(7)(8)(9). Two schemes of measurements can be used: homodyne (selective recording of the light scattered by the system) and heterodyne (recording of beats between the scattered light and reference fixed high-intensity light).…”