Dynamic Lipidomic Insights into the Adaptive Responses of<i>Saccharomyces cerevisiae</i>to the Repeated Vacuum Fermentation

Zhou, Jian; Tian, Hong-Chi; Yang, Yuan

doi:10.1089/omi.2010.0016

Cited by 8 publications

(2 citation statements)

References 54 publications

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“…With the advancement of many new technologies, various omics analysis, such as genomics [ 14 ], lipidomics [ 15 ], proteomics [ 16 ], and metabolomics [ 17 ], have been considered a pivotal tool to help us understand molecular mechanism response to the changes of environment condition. It is reported that DHA is synthesized by a special polyketide synthase (PKS) system in Schizochytrium sp.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and gene expression analysis of docosahexaenoic acid producer Schizochytrium sp. under different oxygen supply conditions

Ren

et al. 2018

Biotechnol Biofuels

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BackgroundSchizochytrium sp. is a promising strain for the production of docosahexaenoic acid (DHA)-rich oil and biodiesel, and has been widely used in the food additive and bioenergy industries. Oxygen is a particularly important environmental factor for cell growth and DHA synthesis. In general, higher oxygen supply favors lipid accumulation, but could lead to a reduction of the DHA percentage in total fatty acids in Schizochytrium sp. To tackle this problem, it is essential to understand the mechanisms regulating the response of Schizochytrium sp. to oxygen. In this study, we aimed to explore the acclimatization of this DHA producer to different oxygen supply conditions by examining the transcriptome changes.ResultsTwo different fermentation processes, namely normal oxygen supply condition (shift agitation speeds from 400 rpm to 300 rpm) and high oxygen supply condition (constant agitation speeds: 400 rpm), were designed to study how the fermentation characteristics of Schizochytrium sp. HX-308 were affected by different oxygen supply conditions. The results indicated that high oxygen supply condition resulted in 49% and 37.5% improvement in the maximum cell dry weight (CDW) and total lipid concentration, respectively. However, the DHA percentage in total fatty acids decreased to 35%, which was 31.4% lower than that produced by normal oxygen supply condition. Moreover, transcriptome analysis was performed to explore the effect of the oxygen supply condition on genetic expression and metabolism. The results showed that glycolysis and pentose phosphate pathway metabolism-associated genes (hexokinase, phosphofructokinase, fructose-bisphosphate aldolase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase) were substantially upregulated in response to high oxygen supply, resulting in more NADPH was available for Schizochytrium. Specially, high oxygen supply condition also led to genes (Δ6 desaturase, Δ12 desaturase, FAS, ORFA, ORFB, and ORFC) involved in fatty acid biosynthesis upregulation. In addition, a transcriptional upregulation of catalase (CAT) became apparent under high oxygen supply condition, while superoxide dismutase (SOD) and ascorbate peroxidase (APX) were found to be down-regulated.ConclusionsThis study is the first to investigate the differences of gene expression at different levels of oxygen availability in the DHA producer Schizochytrium. The results of transcriptome analyses indicated that high oxygen supply condition resulting in more NADPH and acetyl-CoA production for cell growth and lipid synthesis in Schizochytrium. Δ12 desaturase and ORFC showed higher expression levels at high oxygen supply condition, which might be the key regulators for enhancing fatty acid biosynthesis in the future. These results enrich the current knowledge regarding genetic expression and provide important information to enhance DHA production in Schizochytrium sp.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1250-5) contains supplementary material, whi...

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Section: Introductionmentioning

confidence: 99%

Transcriptome and gene expression analysis of docosahexaenoic acid producer Schizochytrium sp. under different oxygen supply conditions

Ren

et al. 2018

Biotechnol Biofuels

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“…When the initial glucose was completely spent, feeding was initiated with a solution containing 500 g/L glucose, 5.12 g/L MgSO 4 ·7H 2 O, 0.28 g/L Na 2 SO 4 , 3.5 g/L K 2 SO 4 , 9 g/L KH 2 PO 4 , 0.6 g/L uracil, 0.5 g/L adenine, 1.2 g/L lysine, 12 ml vitamin solution and 10 ml trace element solution. As previously reported, the vitamin and trace element solution was prepared ( Zhou et al, 2010 ). When glucose consumption was complete and the ethanol produced in the process of metabolism was consumed, feeding with ethanol (95%, V/V) was initiated and the ethanol concentration in the fermenter was kept at 1–6 g/L in addition to 350 ml of feeding solution components other than glucose was added.…”

Section: Methodsmentioning

confidence: 99%

Metabolic Engineering of Saccharomyces cerevisiae for Heterologous Carnosic Acid Production

Wei

Zhang

Bian

et al. 2022

Front. Bioeng. Biotechnol.

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Carnosic acid (CA), a phenolic tricyclic diterpene, has many biological effects, including anti-inflammatory, anticancer, antiobesity, and antidiabetic activities. In this study, an efficient biosynthetic pathway was constructed to produce CA in Saccharomyces cerevisiae. First, the CA precursor miltiradiene was synthesized, after which the CA production strain was constructed by integrating the genes encoding cytochrome P450 enzymes (P450s) and cytochrome P450 reductase (CPR) SmCPR. The CA titer was further increased by the coexpression of CYP76AH1 and SmCPR ∼t28SpCytb5 fusion proteins and the overexpression of different catalases to detoxify the hydrogen peroxide (H2O2). Finally, engineering of the endoplasmic reticulum and cofactor supply increased the CA titer to 24.65 mg/L in shake flasks and 75.18 mg/L in 5 L fed-batch fermentation. This study demonstrates that the ability of engineered yeast cells to synthesize CA can be improved through metabolic engineering and synthetic biology strategies, providing a theoretical basis for microbial synthesis of other diterpenoids.

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Omics in Zebrafish Teratogenesis

Piña

Navarro

Barata

et al. 2018

Methods in Molecular Biology

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The genome revolution represents a complete change on our view of biological systems. The quantitative determination of changes in all major molecular components of the living cells, the "omics" approach, opened whole new fields for all health sciences. Genomics, transcriptomics, proteomics, metabolomics, and others, together with appropriate prediction and modeling tools, will mark the future of developmental toxicity assessment both for wildlife and humans. This is especially true for disciplines, like teratology, which rely on studies in model organisms, as studies at lower levels of organization are difficult to implement. Rodents and frogs have been the favorite models for studying human reproductive and developmental disorders for decades. Recently, the study of the development of zebrafish embryos (ZE) is becoming a major alternative tool to adult animal testing. ZE intrinsic characteristics makes this model a unique system to analyze in vivo developmental alterations that only can be studied applying in toto approaches. Moreover, under actual legislations, ZE is considered as a replacement model (and therefore, excluded from animal welfare regulations) during the first 5 days after fertilization. Here we review the most important components of the zebrafish toolbox available for analyzing early stages of embryotoxic events that could eventually lead to teratogenesis.

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Dynamic Lipidomic Insights into the Adaptive Responses ofSaccharomyces cerevisiaeto the Repeated Vacuum Fermentation

Cited by 8 publications

References 54 publications

Transcriptome and gene expression analysis of docosahexaenoic acid producer Schizochytrium sp. under different oxygen supply conditions

Transcriptome and gene expression analysis of docosahexaenoic acid producer Schizochytrium sp. under different oxygen supply conditions

Metabolic Engineering of Saccharomyces cerevisiae for Heterologous Carnosic Acid Production

Omics in Zebrafish Teratogenesis

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