Dynamic mechanical loading facilitated chondrogenic differentiation of rabbit BMSCs in collagen scaffolds

Cao, Wanxu; Lin, Weimin; Cai, Hanxu; Chen, Yafang; Man, Yi; Liang, Jie; Wang, Qiguang; Sun, Yong; Fan, Yujiang; Zhang, Xingdong

doi:10.1093/rb/rbz005

Cited by 32 publications

(24 citation statements)

References 40 publications

(36 reference statements)

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“…This study showed that dynamic compression significantly enhanced the synthesis of Col II and aggrecan along with an increase of compressive modulus. Cao et al ( 2019 ) seeded rabbit derived MSCs into collagen scaffolds under 10% compressive sinusoidal strain at 1 Hz frequency, for 2 h/day for 21 days. Starting from the second week of culture, the morphology of MSCs in the dynamic culture group exhibited a rounded chondrocyte-like morphology, whereas cells remained spindle shaped in static culture.…”

Section: Mechanical Cues Affecting Stem Cell Differentiationmentioning

confidence: 99%

Influence of the Mechanical Environment on the Regeneration of Osteochondral Defects

Davis

Roldo

Blunn

et al. 2021

Front. Bioeng. Biotechnol.

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Articular cartilage is a highly specialised connective tissue of diarthrodial joints which provides a smooth, lubricated surface for joint articulation and plays a crucial role in the transmission of loads. In vivo cartilage is subjected to mechanical stimuli that are essential for cartilage development and the maintenance of a chondrocytic phenotype. Cartilage damage caused by traumatic injuries, ageing, or degradative diseases leads to impaired loading resistance and progressive degeneration of both the articular cartilage and the underlying subchondral bone. Since the tissue has limited self-repairing capacity due its avascular nature, restoration of its mechanical properties is still a major challenge. Tissue engineering techniques have the potential to heal osteochondral defects using a combination of stem cells, growth factors, and biomaterials that could produce a biomechanically functional tissue, representative of native hyaline cartilage. However, current clinical approaches fail to repair full-thickness defects that include the underlying subchondral bone. Moreover, when tested in vivo, current tissue-engineered grafts show limited capacity to regenerate the damaged tissue due to poor integration with host cartilage and the failure to retain structural integrity after insertion, resulting in reduced mechanical function. The aim of this review is to examine the optimal characteristics of osteochondral scaffolds. Additionally, an overview on the latest biomaterials potentially able to replicate the natural mechanical environment of articular cartilage and their role in maintaining mechanical cues to drive chondrogenesis will be detailed, as well as the overall mechanical performance of grafts engineered using different technologies.

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Section: Mechanical Cues Affecting Stem Cell Differentiationmentioning

confidence: 99%

Influence of the Mechanical Environment on the Regeneration of Osteochondral Defects

Davis

Roldo

Blunn

et al. 2021

Front. Bioeng. Biotechnol.

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“…By altering GelMA concentration, cell proliferation was higher and the gene expression of Col2a1 , Sox9 , and Acan , which were chondrogenic markers, were upregulated with the increase of adhesion ligand density. Similarly, previous research indicated that BMSCs were anchored and required to be attached to the extracellular matrix for survival and proliferation (Cao et al, ). Thus, PEGDA/GelMA hydrogels with more adhesion ligands may provide a more native surface for cellular attachment.…”

Section: Discussionmentioning

confidence: 74%

“…In our study, the 3D scaffold of adhesion ligand density was changed by using different ratios of GelMA in the PEGDA/GelMA hydrogels. Similarly, previous research indicated that BMSCs were anchored and required to be attached to the extracellular matrix for survival and proliferation (Cao et al, 2019). As BMSCs were anchorage dependent, they might primarily be affected by ECM adhesion ligands (Chen, Reuveny, & Oh, 2013;Lu, Lee, Lam, Tabata, & Mikos, 2016).…”

Section: Discussionmentioning

confidence: 84%

Effect of matrix stiffness and adhesion ligand density on chondrogenic differentiation of mesenchymal stem cells

Zhan

2019

J Biomedical Materials Res

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Adhesion ligands and mechanical properties of extracellular matrix (ECM) play significant roles in directing mesenchymal stem cells' (MSCs) behaviors, but how they affect chondrogenic differentiation of MSCs has rarely been studied. In this study, we investigated the effects of matrix stiffness and adhesion ligand density on proliferation and chondrogenic differentiation of MSCs by using UV crosslinked hydrogels comprised of methacrylated gelatin (GelMA) and poly(ethylene glycol) diacrylate (PEGDA) of different weight ratios. The PEGDA/GelMA hydrogels were fabricated by adjusting the weight ratio of PEGDA and GelMA with low or high adhesion ligand density (0.05 and 0.5% GelMA, respectively) and independent tunable stiffness (1.6, 6, and 25 kPa separately for hydrogels with 5, 10, and 15% PEGDA). MSCs presented differential behaviors to ECM by adjusting its adhesion ligand density and stiffness. Cell proliferation and chondrogenic differentiation could be enhanced with the improvement of adhesive properties and stiffness, evidenced by cell viability assay, hematoxylin-eosin staining, Safranin O staining, immunohistochemistry (Collagen types II, Col2a1), as well as the chondrogenic genes expression of Col2a1, Acan, and Sox9. This study may provide new strategies to design the scaffolds for cartilage tissue engineering. K E Y W O R D S adhesion, chondrogenic differentiation, extracellular matrix (ECM), mesenchymal stem cell (MSCs), stiffness

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“…The pH of the collagen solution (2 wt%) was adjusted to about 7 with 1 M NaOH solution. The prepared neutral collagen gel was added with rBMSCs cell suspension and different concentrations of PRP and LyPRP solution to make the final cell concentration reach to 3 × 10 6 cells/ml [ 21 ]. The composite hydrogels blended with rBMSCs were injected into the above-mentioned self-made silicone molds and incubated at 37°C until the gels formed.…”

Section: Methodsmentioning

confidence: 99%

The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

Chen

Liu

et al. 2020

Regenerative Biomaterials

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Although platelet-rich plasma (PRP) plays a significant role in the orthopedic clinical application, it still faces two major problems, namely, uncontrollable factors release, frequent preparation and extraction processes as well as the inconvenient form of usage. To overcome these shortcomings, freeze-dried PRP (LyPRP) was encapsulated into bioactive Col I hydrogel to induce osteogenic differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs). And PRP/Col І composite hydrogel was prepared as a control. Compared with Col І hydrogel, the introduction of platelets significantly improved the mechanical properties of hydrogels. Meanwhile, platelets were evenly distributed in the composite hydrogels network. The sustainable release of related factors in the composite hydrogels could last for more than 14 days to maintain its long-term biological activity. Further cell experiments confirmed that PRP and LyPRP could effectively alleviate the contraction of collagen hydrogel in vitro, and promote the adhesion, proliferation and osteogenesis differentiation of rBMSCs. The results of osteogenic gene expression indicated that the 10% LyPRP/Col І composite hydrogel could facilitate the early expression of BMP-2 and late osteogenic associated protein formation with higher expression of alkaline phosphatase and Osteocalcin (OCN). These results might provide new insights for the clinical application of 10% LyPRP/Col І composite hydrogel as practical bone repair injection.

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Dynamic mechanical loading facilitated chondrogenic differentiation of rabbit BMSCs in collagen scaffolds

Cited by 32 publications

References 40 publications

Influence of the Mechanical Environment on the Regeneration of Osteochondral Defects

Influence of the Mechanical Environment on the Regeneration of Osteochondral Defects

Effect of matrix stiffness and adhesion ligand density on chondrogenic differentiation of mesenchymal stem cells

The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

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