Dynamic metabolic modeling of lipid accumulation and citric acid production by Yarrowia lipolytica

Robles-Rodriguez, C.E.; Bideaux, Carine; Guillouet, Stéphane; Gorret, Nathalie; Cescut, Julien; Uribelarrea, Jean‐Louis; Molina-Jouve, Carole; Roux, G.; Aceves-Lara, Cesar Arturo

doi:10.1016/j.compchemeng.2017.02.013

Cited by 24 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The values of the inhibition constants (Table ) show that the glucose inhibition effect differs in magnitude between citric acid production, lipid accumulation, and growth. The estimated value of the critical quota ( q N * ) was interestingly found to be similar to the critical value of the N/C ratio reported by (Robles‐Rodriguez et al, ).…”

Section: Resultssupporting

confidence: 86%

“…It is known that lipid accumulation in yeast depends on the N/C ratio of the inflow rate of nitrogen and carbon (Beopoulos et al, ; Cescut, ; Ochoa‐Estopier & Guillouet, ; Sitepu et al, ; Ykema et al, ). The critical values for the N/C ratio at which citric acid is produced concomitantly with lipids have been reported at 0.021 mol N C mol −1 (Ochoa‐Estopier & Guillouet, ) for D‐stat experiments, and identified at 0.07 mol N C mol −1 (Robles‐Rodriguez et al, ) after model identification. In addition to the input flow rates, some studies have focused on the changes of the consumption rate of the N/C ratio, which is related to the intracellular change of N/C.…”

Section: Methodsmentioning

confidence: 99%

“…It is noteworthy that the functional biomass contains a part of lipids, which are components of the membrane. These lipids are called structural lipids X LS and are intrinsic to the functional biomass in a constant fraction γ equivalent to 0.06 g X L g X f −1 , (Robles‐Rodriguez et al, ). This definition allows to accounting for a separate term of accumulated lipids X LA .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Modeling and optimization of lipid accumulation by Yarrowia lipolytica from glucose under nitrogen depletion conditions

Robles-Rodriguez

Muñoz-Tamayo

Bideaux

et al. 2018

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

Oleaginous yeasts have been seen as a feasible alternative to produce the precursors of biodiesel due to their capacity to accumulate lipids as triacylglycerol having profiles with high content of unsaturated fatty acids. The yeast Yarrowia lipolytica is a promising microorganism that can produce lipids under nitrogen depletion conditions and excess of the carbon source. However, under these conditions, this yeast also produces citric acid (overflow metabolism) decreasing lipid productivity. This work presents two mathematical models for lipid production by Y. lipolytica from glucose. The first model is based on Monod and inhibition kinetics, and the second one is based on the Droop quota model approach, which is extended to yeast. The two models showed good agreements with the experimental data used for calibration and validation. The quota based model presented a better description of the dynamics of nitrogen and glucose dynamics leading to a good management of N/C ratio which makes this model interesting for control purposes. Then, quota model was used to evaluate, by means of simulation, a scenario for optimizing lipid productivity and lipid content. For that, a control strategy was designed by approximating the flow rates of glucose and nitrogen with piecewise linear functions. Simulation results achieved productivity of 0.95 g L hr and lipid content fraction of 0.23 g g , which indicates that this strategy is a promising alternative for the optimization of lipid production.

show abstract

Section: Resultssupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Modeling and optimization of lipid accumulation by Yarrowia lipolytica from glucose under nitrogen depletion conditions

Robles-Rodriguez

Muñoz-Tamayo

Bideaux

et al. 2018

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, Robles-Rodriguez et al (2017) came up with three dynamic metabolic models, based on a simplified metabolic network, for describing lipid accumulation and citric acid production by Y. lipolytica growing on glucose. These models can guide the design of strategies for improving culture performance via the identification of rate-controlling steps and metabolic fluxes.…”

Section: Computational Toolsmentioning

confidence: 99%

Synthetic biology tools for engineering Yarrowia lipolytica

Larroude

Rossignol

Nicaud

et al. 2018

Biotechnology Advances

135

View full text Add to dashboard Cite

The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools.

show abstract

“…These values fitted the model predictions and were superior to previous reports for continuous cultures. Additionally, three dynamic metabolic models of Y. lipolytica were presented to describe its lipid accumulation and citric acid production ( Robles-Rodriguez et al, 2017 ). Results showed a good fit of parameters on describing the dynamics of lipids and citric acid production, which proved that they can be incorporated into control strategies to optimize lipid accumulation.…”

Section: Development Of Tools and Approaches For Production Of Fuels mentioning

confidence: 99%

Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals

Shi

Zhao

2017

Front. Microbiol.

View full text Add to dashboard Cite

Oleaginous yeasts have been increasingly explored for production of chemicals and fuels via metabolic engineering. Particularly, there is a growing interest in using oleaginous yeasts for the synthesis of lipid-related products due to their high lipogenesis capability, robustness, and ability to utilize a variety of substrates. Most of the metabolic engineering studies in oleaginous yeasts focused on Yarrowia that already has plenty of genetic engineering tools. However, recent advances in systems biology and synthetic biology have provided new strategies and tools to engineer those oleaginous yeasts that have naturally high lipid accumulation but lack genetic tools, such as Rhodosporidium, Trichosporon, and Lipomyces. This review highlights recent accomplishments in metabolic engineering of oleaginous yeasts and recent advances in the development of genetic engineering tools in oleaginous yeasts within the last 3 years.

show abstract

Dynamic metabolic modeling of lipid accumulation and citric acid production by Yarrowia lipolytica

Cited by 24 publications

References 31 publications

Modeling and optimization of lipid accumulation by Yarrowia lipolytica from glucose under nitrogen depletion conditions

Modeling and optimization of lipid accumulation by Yarrowia lipolytica from glucose under nitrogen depletion conditions

Synthetic biology tools for engineering Yarrowia lipolytica

Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals

Contact Info

Product

Resources

About