Dynamic metabolomic responses of <i>Escherichia coli</i> to nicotine stress

Ding, Lijian; Chen, Juanjuan; Zou, Jianding; Zhang, Limin; Ye, Yangfang

doi:10.1139/cjm-2014-0206

Cited by 7 publications

(10 citation statements)

References 39 publications

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“…2, 4a). Nicotine, 12.34 mM or higher, alters energy-related metabolism of E. coli, including changes to glycolysis and inhibition of the tricarboxylic acid cycle (Ding et al 2014). As discussed in the introduction, nicotine can both stimulate and inhibit bacterial growth, depending on the concentration applied (Huang et al 2012;Zaidi et al 2012).…”

Section: Discussionmentioning

confidence: 99%

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Investigation of feeding behaviour in C. elegans reveals distinct pharmacological and antibacterial effects of nicotine

Kudelska

Lewis

et al. 2018

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Caenorhabditis elegans is an informative model to study the neural basis of feeding. A useful paradigm is one in which adult nematodes feed on a bacterial lawn which has been pre-loaded with pharmacological agents and the effect on pharyngeal pumping rate scored. A crucial aspect of this assay is the availability of good quality bacteria to stimulate pumping to maximal levels. A potential confound is the possibility that the pharmacological agent impacts bacterial viability and indirectly influences feeding rate. Here, the actions of nicotine on pharyngeal pumping of C. elegans and on the Escherichia coli bacterial food source were investigated. Nicotine caused an immediate and concentration-dependent inhibition of C. elegans pharyngeal pumping, IC 50 4 mM (95% CI = 3.4 mM to 4.8 mM). At concentrations between 5 and 25 mM, nicotine also affected the growth and viability of E. coli lawns. To test whether this food depletion by nicotine caused the reduced pumping, we modified the experimental paradigm. We investigated pharyngeal pumping stimulated by 10 mM 5-HT, a food 'mimic', before testing if nicotine still inhibited this behaviour. The IC 50 for nicotine in these assays was 2.9 mM (95% CI = 3.1 mM to 5.1 mM) indicating the depletion of food lawn does not underpin the potency of nicotine at inhibiting feeding. These studies show that the inhibitory effect of nicotine on C. elegans pharyngeal pumping is mediated by a direct effect rather than by its poorly reported bactericidal actions.

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Section: Discussionmentioning

confidence: 99%

“…Moreover, two novel nicotine analogues had antibacterial properties against E. coli when applied at 6 mM (Gandhi and Athmaram 2016; Bautista et al 2014). Nicotine at low mM concentrations changes the energy-related metabolism and choline metabolism of E. coli, resulting in what the authors call nicotine stress (Ding et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Investigation of feeding behaviour in C. elegans reveals distinct pharmacological and antibacterial effects of nicotine

Kudelska

Lewis

et al. 2018

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“…The native role(s) of CYP6CY3 in the aphid bacteriocyte is unclear. However, in relation to the host shift of M. persicae to tobacco, it is notable that nicotine has strong antimicrobial properties extending to Gram-negative bacteria such as Escherichia coli (12,13), which is the closest free-living relative of B. aphidicola (11). This led to a further question: Does the up-regulation of CYP6CY3 in M. p. nicotianae protect B. aphidicola from the inhibitory effects of nicotine?…”

Section: Spatial Expression Of Cyp6cy3 Is Key To Fitnessmentioning

confidence: 99%

The genetic architecture of a host shift: An adaptive walk protected an aphid and its endosymbiont from plant chemical defenses

et al. 2020

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Host shifts can lead to ecological speciation and the emergence of new pests and pathogens. However, the mutational events that facilitate the exploitation of novel hosts are poorly understood. Here, we characterize an adaptive walk underpinning the host shift of the aphid Myzus persicae to tobacco, including evolution of mechanisms that overcame tobacco chemical defenses. A series of mutational events added as many as 1.5 million nucleotides to the genome of the tobacco-adapted subspecies, M. p. nicotianae, and yielded profound increases in expression of an enzyme that efficiently detoxifies nicotine, both in aphid gut tissue and in the bacteriocytes housing the obligate aphid symbiont Buchnera aphidicola. This dual evolutionary solution overcame the challenge of preserving fitness of a mutualistic symbiosis during adaptation to a toxic novel host. Our results reveal the intricate processes by which genetic novelty can arise and drive the evolution of key innovations required for ecological adaptation.

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“…However, during the lycopene fermentation with nicotine, few amino acids and their derivatives were detected, suggesting that nicotine decreases the efficiency of protein synthesis. Ding et al (2014) also reported that nicotine decreases the amino acid concentration. This effect of nicotine may also contribute to fungal morphological changes.…”

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confidence: 93%

“…Phosphoric acid, which is usually important for adjusting signal transduction pathways, also contributes to the conversion of adenosine diphosphate (ADP) to adenosine triphosphate (ATP), the oxidation of citrate in the TCA cycle, and glycolysis (Ding et al, 2014). In the current study, an analysis of phosphoric acid (Fig.…”

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confidence: 99%