Dynamic modelling of growth and flavonoid production from Ocimum tenuiflorum suspension culture

Jacob, Anu; Mahanty, Biswanath; Thomas, Jibu

doi:10.1007/s00449-020-02394-6

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…Our results are consistent with earlier research that has documented the promotive impact of Phe on both plant growth and secondary metabolite production [51]. The stimulatory influence of Phe on flavonoid biosynthesis, like in Ocimum tenuiflorum and Mentha piperita plants, has been documented by studies conducted by Jacob and Thomas [54] and Roy et al [55]. Among the flavonoid compounds, quersestin was present in the highest quantity, while rutin was found in the lowest amount.…”

Section: Discussionsupporting

confidence: 93%

Enhancing Growth, Yield, and Antioxidant Activity of Bitter Gourd (Momordica charantia L.) through Amino Acid Foliar Spray Application

El-Khayat,

Elbagory,

Elsadek

et al. 2023

Horticulturae

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Bitter gourd has picked up noteworthy consideration for its pharmacological benefits. However, the impact of amino acids (AAs) dosage on growth, yield, and antioxidants is uncertain. In this study, we investigated the effects of foliar spraying bitter gourd with 100, 200, and 300 mg/L dosages of tryptophan (Trp), glutamine (Gln), and phenylalanine (Phe). The results revealed that Trp, at a dosage of 300 mg/L, produced the most substantial increase in plant length, followed by 300 mg/L Phe. Additionally, the highest values of the fresh dry weight of the plants, fresh weight of the first fruit, fruit number per plant, fresh weight of the fruits per plant, and total fruit output per hectare were seen at 300 mg/L of Trp, followed by 300 mg/L of Gln. Phe at 300 mg/L yielded the highest levels of total phenolics and total flavonoids, coupled with strong scavenging activity against 2,2-diphenyl-1-picrylhydrazyl. These outcomes show the potential of Trp and Gln foliar sprays to enhance bitter gourd growth, yield, and certain antioxidant compounds. These findings carry substantial implications for the enhancement of bitter gourd cultivation and quality. By revealing AA’s potential for improving bitter gourd, our research contributes to bolstering the agricultural sustainability of this remarkable crop.

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Section: Discussionsupporting

confidence: 93%

Enhancing Growth, Yield, and Antioxidant Activity of Bitter Gourd (Momordica charantia L.) through Amino Acid Foliar Spray Application

El-Khayat,

Elbagory,

Elsadek

et al. 2023

Horticulturae

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“…The model assumes an irreversible progression in which active cells (active DM) are converted into dead cells (dead DM) ( Jacob et al., 2020 ) (Eq. 2).…”

Section: Resultsmentioning

confidence: 99%

“…A mechanistic, kinetic modeling approach is preferred for PCCs, where all metabolic effects are lumped into unstructured kinetic functions for simplification ( Resat et al., 2009 ), especially for model-based DoE where the number of experiments is small and hence only few model parameters can be fitted. In particular, Monod-type models are preferred because they simulate nutrient uptake, biomass formation and protein production while differentiating between viable and dead cells ( Prakash and Srivastava, 2006 ; Prakash and Srivastava, 2008 ; Jiménez-Hornero et al., 2009a ; Jiménez-Hornero et al., 2009b ; Puad et al., 2017 ; Villegas et al., 2017 ; Puad and Abdullah, 2018 ; Jacob et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Simulation and optimization of nutrient uptake and biomass formation using a multi-parameter Monod-type model of tobacco BY-2 cell suspension cultures in a stirred-tank bioreactor

Nausch,

Baldan,

Teichert

et al. 2023

Front. Plant Sci.

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IntroductionTobacco (Nicotiana tabacum) cv Bright Yellow-2 (BY-2) cell suspension cultures enable the rapid production of complex protein-based biopharmaceuticals but currently achieve low volumetric productivity due to slow biomass formation. The biomass yield can be improved with tailored media, which can be designed either by laborious trial-and-error experiments or systematic, rational design using mechanistic models, linking nutrient consumption and biomass formation.MethodsHere we developed an iterative experiment-modeling-optimization workflow to gradually refine such a model and its predictions, based on collected data concerning BY-2 cell macronutrient consumption (sucrose, ammonium, nitrate and phosphate) and biomass formation. Results and discussionThe biomass formation was well predicted by an unstructured segregated mechanistic Monod-type model as long as the nutrient concentrations did not approach zero (we omitted phosphate, which was completely depleted). Multi-criteria optimization for sucrose and biomass formation indicated the best tradeoff (in a Paretian sense) between maximum biomass yield and minimum process time by reducing the initial sucrose concentration, whereas the inoculation biomass could be increased to maximize the biomass yield or minimize the process time, which we confirmed in calibration experiments. The model became inaccurate at biomass densities > 8 g L-1 dry mass when sucrose was almost depleted. We compensated for this limitation by including glucose and fructose as sucrose hydrolysis products in the model. The remaining offset between the simulation and experimental data might be resolved by including intracellular pools of sucrose, ammonium, nitrate and phosphate. Overall, we demonstrated that iterative models can be used to systematically optimize conditions for bioreactor-based processes.

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Dynamic modelling of growth and flavonoid production from Ocimum tenuiflorum suspension culture

Cited by 2 publications

References 42 publications

Enhancing Growth, Yield, and Antioxidant Activity of Bitter Gourd (Momordica charantia L.) through Amino Acid Foliar Spray Application

Enhancing Growth, Yield, and Antioxidant Activity of Bitter Gourd (Momordica charantia L.) through Amino Acid Foliar Spray Application

Simulation and optimization of nutrient uptake and biomass formation using a multi-parameter Monod-type model of tobacco BY-2 cell suspension cultures in a stirred-tank bioreactor

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