Dynamic modulation of matrix adhesiveness induces epithelial-to-mesenchymal transition in prostate cancer cells in 3D

Pol, M.A. van der; Gao, Hanyuan; Zhang, He; George, Olivia J; Fox, Joseph M.; Jia, Xinqiao

doi:10.1016/j.biomaterials.2023.122180

Cited by 8 publications

(2 citation statements)

References 85 publications

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“…Recently, peptide decoration to HA was at the basis of the fabrication of a tunable hydrogel platform having protease susceptibility and cell adhesive properties employing bioorthogonal inverse electron tetrazine ligation with strained alkenes like trans-cyclooctene (TCO). [79] In this study, tetrazinefunctionalized HA (HA-Tz) was first crosslinked by a bisnorbornene-functionalized protease susceptible peptide used as crosslinker to obtain the hydrogel. Then the hydrogel was modified via a diffusion-controlled method using a transcyclooctene-functionalized cell adhesive peptide reacting to HA-Tz by cycloaddition (Figure 9).…”

Section: Peptide Bioconjugation By Bioorthogonal Chemistrymentioning

confidence: 99%

Chemical Modifications in Hyaluronic Acid‐Based Electrospun Scaffolds

Pepe,

Laezza,

Armiento

et al. 2024

ChemPlusChem

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Hyaluronic acid (HA) is a natural, non‐sulfated glycosaminoglycan (GAG) present in ECM. It is involved in different biological functions with appealing properties in cosmetics and pharmaceutical preparations as well as in tissue engineering. Generally, HA has been electrospun in blends with natural or synthetic polymers to produce fibers having diameters in the order of nano and micro‐scale whose pores can host cells able to regenerate damaged tissues. In the last decade, a rich literature on electrospun HA‐based materials arose. Chemical modifications were generally introduced in HA scaffolds to favour crosslinking or conjugation with bioactive molecules. Considering the high solubility of HA in water, HA‐based electrospun scaffolds are cross‐linked to increase the stability in biological fluids. Crosslinking is necessary also to avoid the release of HA from the hybrid scaffold when implanted in‐vivo. Furthermore, to endow the HA based scaffolds with new chemical or biological properties, conjugation of bioactive molecules to HA was widely reported. Herein, we review the existing research classifying chemical modifications on HA and HA‐based electrospun fibers into three categories: i) in‐situ crosslinking of electrospun HA‐based scaffolds ii) off‐site crosslinking of electrospun HA‐based scaffolds; iii) conjugation of biofunctional molecules to HA with focus on peptides.

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Section: Peptide Bioconjugation By Bioorthogonal Chemistrymentioning

confidence: 99%

Chemical Modifications in Hyaluronic Acid‐Based Electrospun Scaffolds

Pepe,

Laezza,

Armiento

et al. 2024

ChemPlusChem

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“…21–23 The versatility and predictability of this chemistry have also made it useful for conjugating drugs to antibodies, 24 as well as preparing novel polymers and designing surface reactions. 25–27 Indeed, the IEDDA reaction between tetrazine and trans -cyclooctene (TCO) has been used for extracellular vesicles enrichment. 28,29…”

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confidence: 99%

A bioorthogonal cell sorting strategy for isolation of desired cell phenotypes

Dai,

Zhou,

Yang

et al. 2024

Chem. Commun.

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Modeling the Maturation of the Vocal Fold Lamina Propria Using a Bioorthogonally Tunable Hydrogel Platform

Zou,

Zhang,

Benson

et al. 2023

Adv Healthcare Materials

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Toward the goal of establishing an engineered model of the vocal fold lamina propria (LP), mesenchymal stem cells (MSCs) were encapsulated in hyaluronic acid (HA)‐based hydrogels employing tetrazine ligation with strained alkenes. To mimic matrix stiffening during LP maturation, diffusion‐controlled interfacial bioorthogonal crosslinking was carried out on the soft cellular construct using HA modified with a ferocious dienophile, trans‐cyclooctene (TCO). Cultures were maintained in MSC growth media for 14 days to afford a model of a newborn LP that is homogeneously soft (nLP), a homogeneously stiffened construct zero (sLP0) or 7 days (sLP7) post cell encapsulation, and a mature LP model (mLP) with a stiff top layer and a soft bottom layer. Installation of additional HA crosslinks restricted cell spreading. Compared to the nLP controls, sLP7 conditions upregulated the expression of fibrous matrix proteins (Col I, DCN, and FN EDA), classic fibroblastic markers (TNC, FAP, and FSP1), and matrix remodeling enzymes (MMP2, TIMP1, and HAS3). Day 7 stiffening also upregulated the catabolic activities, enhanced ECM turnover, and promoted YAP expression. Overall, in situ delayed matrix stiffening promoted a fibroblast transition from MSCs and enhanced YAP‐regulated mechanosensing.This article is protected by copyright. All rights reserved

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Dynamic modulation of matrix adhesiveness induces epithelial-to-mesenchymal transition in prostate cancer cells in 3D

Cited by 8 publications

References 85 publications

Chemical Modifications in Hyaluronic Acid‐Based Electrospun Scaffolds

Chemical Modifications in Hyaluronic Acid‐Based Electrospun Scaffolds

A bioorthogonal cell sorting strategy for isolation of desired cell phenotypes

Modeling the Maturation of the Vocal Fold Lamina Propria Using a Bioorthogonally Tunable Hydrogel Platform

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