Dynamic monitoring of oscillatory enzyme activity of individual live bacteria via nanoplasmonic optical antennas

Lu, Dengyun; Zhu, Guoshuai; Li, Xing; Xiong, Jianyun; Wang, Danning; Shi, Yang; Pan, Ting; Li, Baojun; Lee, Luke P.; Xin, Hongbao

doi:10.1038/s41566-023-01265-2

Cited by 17 publications

(11 citation statements)

References 54 publications

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“…The plasmon resonance tuning in the visible can be effectively performed via designing aluminum nanodisk array geometries . However, future metasurface-based studies could explore the potential of metasurface to analyze quantum biological effects with present spatial information, which could be applied for cancer therapeutics, cell life cycle monitoring, and dynamic monitoring of enzyme activity of live bacteria or cells …”

Section: Discussionmentioning

confidence: 99%

“…51 However, future metasurfacebased studies could explore the potential of metasurface to analyze quantum biological effects with present spatial information, which could be applied for cancer therapeutics, 50 cell life cycle monitoring, 48 and dynamic monitoring of enzyme activity of live bacteria or cells. 102 We also highlight current advances in monitoring cell morphology, secretion, and tissue section analysis as diagnostic methods. Metasurfaces made of gold or amorphous Si are mainly used for cell monitoring due to their optical properties in visible or near-infrared regions, biocompatibility, and wellknown surface modification methods.…”

Section: ■ Conclusionmentioning

confidence: 99%

See 1 more Smart Citation

Metasurfaces for Quantitative Biosciences of Molecules, Cells, and Tissues: Sensing and Diagnostics

Barulin,

Nguyen,

Kim

et al. 2024

ACS Photonics

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Metasurfaces have revolutionized optical biosensing and diagnostic assays due to their sensitivity, compactness, and label-free operation. However, metasurface applications in analyzing complex biological systems and quantum bioscience phenomena remain scarce. In this Perspective, we discuss current developments in metasurface biosensors and propose promising future applications for probing or adopting quantum bioscience effects and improving spatial omics analysis in live cells and tissues. We discuss the capabilities of the current metasurface platforms for monitoring relevant biomarkers of viral diseases, neurodegenerative diseases, and cancers. Metasurfaceempowered examination of cell morphology and secretome, virus detection, and tissue imaging can improve the accuracy of early diagnosis. Furthermore, we review device-integration approaches for point-of-care testing settings that could constitute pathways toward technology commercialization. Altogether, we provide a perspective for exploring metasurface applications in precision health and medicine.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

Metasurfaces for Quantitative Biosciences of Molecules, Cells, and Tissues: Sensing and Diagnostics

Barulin,

Nguyen,

Kim

et al. 2024

ACS Photonics

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“…Moreover, the plasmonic gold nanoparticle-based nano-probes have contributed a lot to biological study. [73][74][75][76] However, achieving precise simultaneous manipulation across microscale bioparticles and nanoparticles remains challenging. HAONT can also promote the interactions between cells and AuNS and gather them into a biocompatible assembly.…”

Section: Nanoparticle Trapping and Assemblingmentioning

confidence: 99%

“…For instance, the precise interaction between MSNs and cells holds the potential for accurate single-cell drug delivery. Immobilizing metal nanoparticles on bacterial surfaces, such as E. coli cells, enables in situ surface-enhanced Raman scattering (SERS) applications for detecting biological differences at a sub-cellular level, [80] inter-bacteria communications, [76] or antibacterial applications. Moreover, HAONT facilitates the manipulation of DNA or RNA molecules through diffusiophoresis, [49] allowing for simultaneous trapping and bioreactions.…”

Section: Doughnut-shaped Vortex Trapping and Biological Applicationsmentioning

confidence: 99%

Highly‐Adaptable Optothermal Nanotweezers for Trapping, Sorting, and Assembling across Diverse Nanoparticles

Chen,

Zhou,

Peng

et al. 2023

Advanced Materials

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Optical manipulation of various kinds of nanoparticles is vital in biomedical engineering. However, classical optical approaches demand higher laser power and are constrained by diffraction limits, necessitating tailored trapping schemes for specific nanoparticles. They lack a universal and biocompatible tool to manipulate nanoparticles of diverse sizes, charges, and materials. Through precise modulation of diffusiophoresis and thermo‐osmotic flows in the boundary layer of an optothermal‐responsive gold film, highly adaptable optothermal nanotweezers (HAONTs) capable of manipulating a single nanoparticle as small as sub‐10 nm are designed. Additionally, a novel optothermal doughnut‐shaped vortex (DSV) trapping strategy is introduced, enabling a new mode of physical interaction between cells and nanoparticles. Furthermore, this versatile approach allows for the manipulation of nanoparticles in organic, inorganic, and biological forms. It also offers versatile function modes such as trapping, sorting, and assembling of nanoparticles. It is believed that this approach holds the potential to be a valuable tool in fields such as synthetic biology, optofluidics, nanophotonics, and colloidal science.

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“…Our study unveils the unprecedented capability of the optical meron in manipulating nanoparticles in both a still and a fluid environment. It also opens the way for exploiting extraordinary topological textures in dielectric nanostructures for numerous on-chip optofluidic applications, 58 such as biosensing, 62 screening, and biointeractions. 63 ■ METHODS Calculation of Optical Forces.…”

mentioning

confidence: 99%

Nanoparticle Deep-Subwavelength Dynamics Empowered by Optical Meron–Antimeron Topology

Lu,

Wang,

Fang

et al. 2023

Nano Lett.

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Optical meron is a type of nonplanar topological texture mainly observed in surface plasmon polaritons and highly symmetric points of photonic crystals in the reciprocal space. Here, we report Poynting-vector merons formed at the real space of a photonic crystal for a Γ-point illumination. Optical merons can be utilized for subwavelength-resolution manipulation of nanoparticles, resembling a topological Hall effect on electrons via magnetic merons. In particular, staggered merons and antimerons impose strong radiation pressure on large gold nanoparticles (AuNPs), while focused hot spots in antimerons generate dominant optical gradient forces on small AuNPs. Synergistically, differently sized AuNPs in a still environment can be trapped or orbit in opposite directions, mimicking a coupled galaxy system. They can also be separated with a 10 nm precision when applying a flow velocity of >1 mm/s. Our study unravels a novel way to exploit topological textures for optical manipulation with deep-subwavelength precision and switchable topology in a lossless environment.

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Dynamic monitoring of oscillatory enzyme activity of individual live bacteria via nanoplasmonic optical antennas

Cited by 17 publications

References 54 publications

Metasurfaces for Quantitative Biosciences of Molecules, Cells, and Tissues: Sensing and Diagnostics

Metasurfaces for Quantitative Biosciences of Molecules, Cells, and Tissues: Sensing and Diagnostics

Highly‐Adaptable Optothermal Nanotweezers for Trapping, Sorting, and Assembling across Diverse Nanoparticles

Nanoparticle Deep-Subwavelength Dynamics Empowered by Optical Meron–Antimeron Topology

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