Dynamic Multivalency for Carbohydrate–Protein Recognition through Dynamic Combinatorial Libraries Based on Fe^II–Bipyridine Complexes

Abstract

Molecular recognition: Dynamic combinatorial libraries (DCLs) exploiting multivalency effects and metal coordination have been employed for carbohydrate-protein recognition. The interaction of a three-component DCL based on 2,2'-bipyridine (bipy)-Fe(II) complexes with concanavalin A (ConA; see scheme) results in enhanced binding by multivalent presentation with a bias towards mannose-containing library components.

Metal complexes as “protein surface mimetics”

“…141 De Mendoza and co-workers used bipyridines functionalised with 3 different sugars complexed them to Fe(II) then incubated them with the mannose binding lectin, Concanavalin A (ConA), this resulted in enrichment of the mannose functionalised complex (detected by LCMS), as predicted. 142 Please do not adjust margins Please do not adjust margins While the labile nature of the Fe(II) core can be useful for the generation of high affinity protein receptors, the inert nature of the ruthenium(II) core is attractive as decomplexation will not occur in biological media in dilute solution. 143 Moreover, the ruthenium(II) core permits detection of binding events through the metal to ligand charge transfer (MLCT) luminescence.…”

Switching Multivalent DNA Complexation using Metal‐Controlled Cationic Supramolecular Self‐Assemblies

“…These events epigenetically mediate the condensation of nucleic acidsa nd thereby modulate gene expression. [12] Grid-type metal complexes have been extensively developed,m ostly for applications in material science and data storage. [3] The first challenge in this application is to condense therapeutic oligonucleotides (siRNA,m iRNA, etc) into small nanoparticles that are effectively taken up by cells.…”

“…This process of controlledn ucleic acid recognition and condensation is also crucial in functional nucleic acidd elivery applications. [12] Grid-type metal complexes have been extensively developed,m ostly for applications in material science and data storage. Numerous cationic macromolecules( polymers, dendrimers) have been developed as synthetic gene delivery vehicles.…”

Dynamic combinatorial chemistry: a tool to facilitate the identification of inhibitors for protein targets

“…73 Previously, a prototype DCL was reported by Sasaki consisting of four stereoisomers of a homoleptic Fe(II)-bipy complex for lectin (carbohydrate-binding protein) recognition. In 2013, the group of de Mendoza demonstrated for the first time that multicomponent exchanging DCLs of Fe(II)-bipyridine (Fe(II)-bipy) complexes can be used for carbohydrate-protein recognition studies.…”